DNA sequencing determines the precise order of nucleotides- adenine, guanine, cytosine and thymine, within the DNA molecule. The 1970’s technologies of Sanger’s chain termination, and Maxim and Gilbert’s chain degradation, were improved upon and automated with ABI’s fully automated sequencing machine in 1987. Since that time sequencing has gotten faster and cheaper; next generation sequencing has taken it to the level of complete genome sequencing in a matter of hours. No matter the method, DNA template needs to be isolated and purified, and reagents such as primers and enzymes need to be purchased. Laboratories requiring sequences to further scientific research, make treatment decisions, or use in genetic counseling will find the products they need here available for comparison.
Early-career scientists share experimental details and more.
The run down on optimized reagents, kits, and workstations.
Short Tandem Repeats (STRs) are accepted markers for human forensic analysis and...
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