Comparing Fluorescence and Trypan Blue Automated Cell Counting

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. The use of Trypan Blue dye can present challenges for accurately assessing cell counts and viability in some sample types. For these samples, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative. Using the CellDrop Automated Cell Counter, DeNovix Scientific Director Dan Schieffer demonstrates both brightfield and fluorescence methods of cell counting and viability assessment.

November 12, 2021

Scientific Director
Dan joined NanoDrop Technologies Inc. in 2006 while completing his BS in Biotechnology at University of Delaware. After contributing to the development of the NanoDrop 8000 and NanoDrop 2000, Dan accepted a teaching and research assistantship at Villanova University. At Villanova he helped develop a novel flow cytometry-based FRET system to elucidate the role of cell membrane organization in CD4+ T Cell activation. Dan then moved into a research assistantship role at the University of Pennsylvania, where he performed high throughput genetic screens to discover novel immune system pathways that regulate pathogenesis in insect-borne viruses. Dan joined DeNovix in 2013. He has an MS in Immunology from Villanova University.