Neutrophil Extracellular Traps Implicated in Contact Dermatitis

A research team from Japan’s Fujita Health University School of Medicine reports insights into the molecular mechanisms that trigger contact hypersensitivity (CHS), more widely known as contact dermatitis. The common condition is the result of an allergic reaction to substances such as perfumes, household chemicals, and accessories.

The group had previously shown that genetic mutations in the interleukin (IL)36RN gene, which is responsible for the synthesis of the protein interleukin-36 receptor antagonist (or IL-36Ra), may result in enhanced CHS. They also observed that the mutations led to the increased formation of neutrophil extracellular traps (NETs), which are mesh-like structures of DNA and proteins released by neutrophils to capture pathogens. However, the immunological pathophysiology of NETs in CHS remained unclear. On two previous occasions, they also successfully established links between IL-36Ra and psoriatic lesions as well as between IL-36Ra and wound healing.

The most recent study, published in Scientific Reports, shows that NETs could be a potential therapeutic target for CHS. Lead author Dr. Yurie Hasegawa, a graduate student at the Fujita Health University School of Medicine, along with co-authors Dr. Yohei Iwata and Professor Kazumitsu Sugiura, found that NETs play a prominent role in the CHS response in the presence of a high number of inflammatory cells like CD4+ and CD8+ T-cells.

“NETs may also exacerbate immune reactions in CHS. Therefore, the inhibition of NET formation may be a new therapeutic strategy in contact dermatitis,” says Dr. Hasegawa.

To test their hypothesis, the team evaluated NET formation in Il36rn-/- mutant mice as well as wild-type mice under the influence of CHS-responsive CD4+ and CD8+ T cells. They first tested the effects of Cl-amidine on the CHS response in the mutant and wild-type mice and found that the number of infiltrating inflammatory cells and area of NETs decreased in both mice types.

Histopathology and cell assays confirmed that the mutant mice had considerably more macrophages and CD4+ and CD8+ T cells than the wild-type mice. Moreover, they demonstrated elevated levels of inflammatory cytokines like IL-1, CXCL1, CXCL2, IL-17A, tumor necrosis factor-α, and IL-36. Interestingly, there were striking differences in IFN-γ and CXCL1 levels between Cl-amidine-treated mutant mice and untreated wild-type mice, but not in the levels of inflammatory cytokines.

Next, Hasegawa and colleagues used the immunofluorescence staining to show that the mutant mice had more NETs in their ear tissues than the wild-type mice. However, treating mice with Cl-amidine—an inhibitor that suppresses the formation of NETs—resulted in a decreased CHS response, suggesting that NETs could be a potential therapeutic target for CHS.

The team is now looking to further explore the connections between NET formation and CHS treatment. “Our study is the first to suggest the involvement of NETs in the immune responses in CHS. All our findings collectively indicate that the blockade of NET formation by Cl-amidine may be a potential treatment approach for allergic contact dermatitis,” says Dr. Hasegawa.