Humans were enjoying beer and blue cheese 2,700 years ago, according to a study published today in Current Biology. An international team of scientists examining the Austrian UNESCO World Heritage Hallstatt-Dachstein/Salzkammergut area discovered the presence of two fungal species used in the production of blue cheese and beer in ancient feces samples.

Earlier studies already had shown the potential for studies of prehistoric paleofeces from salt mines to offer important insights into early human diet and health. In the new study, the researchers used in-depth microscopic, metagenomic, and proteomic analyses to examine the samples. Their initial dietary survey identified bran and glumes of different cereals as one of the most prevalent plant fragments. They report that this highly fibrous, carbohydrate-rich diet was supplemented with proteins from broad beans and occasionally with fruits, nuts, or animal food products. When the researchers extended their microbial survey to include fungi, that’s when they got their biggest surprise: an abundance in one of their Iron Age samples of Penicillium roqueforti and Saccharomyces cerevisiae DNA. 

“Genome-wide analysis indicates that both fungi were involved in food fermentation and provide the first molecular evidence for blue cheese and beer consumption during Iron Age Europe,” says Frank Maixner of the Eurac Research Institute for Mummy Studies in Italy.

Search Antibodies
Search Now Use our Antibody Search Tool to find the right antibody for your research. Filter
by Type, Application, Reactivity, Host, Clonality, Conjugate/Tag, and Isotype.

“The Hallstatt miners seem to have intentionally applied food fermentation technologies with microorganisms which are still nowadays used in the food industry,” Maixner says. The findings offer the first evidence that people were already producing blue cheese in Iron Age Europe nearly 2,700 years ago, he adds. In ongoing and future studies of the paleofeces from Hallstatt, they hope to learn more about the early production of fermented foods and the interplay between nutrition and the gut microbiome composition in different time periods.