Steps in tRNA Methylthiolation Process Outlined

Penn State researchers describe the chemical steps in the methylthiolation process in a paper published in Nature today. By imaging the MiaB protein, which facilitates this RNA modification in bacteria, the team was able to reconstruct the process.

During methylthiolation, the addition of a chemical tag (a methyl sulfur group) to a particular location on some transfer RNAs (tRNA) improves their ability to translate messenger RNA into proteins. When methylthiolation doesn’t occur properly, mistakes can be incorporated into the resulting proteins, which in humans can lead to neuronal disease, cancer, and increased risk of developing Type 2 diabetes.

“Methylthiolation is ubiquitous across bacteria, plants, and animals,” said Squire Booker, who led the research team. “In this study, we determined the structure of a protein called MiaB to better understand its role in facilitating this important modification process in bacteria.”

The MiaB protein from the bacteria Bacteroides uniforms is a member of the radical SAM (S-adenosylmethionine) family of enzymes. Radical SAM enzymes typically use one of their own iron-sulfur clusters to convert a SAM molecule into a free radical that helps move the reaction forward. Unlike most other radical SAM enzymes, MiaB contains two iron-sulfur clusters: a radical SAM cluster and an auxiliary cluster, where most of the intricate chemistry takes place.

Imaging MiaB in action with SAM molecules and tRNA at several points during methylthiolation allowed the researchers to infer the chemical steps during the modification process. First, a molecule of SAM donates its methyl group to the auxiliary iron-sulfur cluster on MiaB. 

“The source of the sulfur atom attached to the tRNA has been controversial, but our structures reveal that a methyl group from SAM attaches to a sulfur atom on MiaB’s auxiliary iron-sulfur cluster,” said Olga Esakova, first author of the paper. “This methyl group and the sulfur it attaches to on MiaB are ultimately what transfers to the tRNA, but some additional steps occur before the tRNA can accept the methylthio group.”

The addition of an electron fragments a second molecule of SAM into a free radical. The radical ultimately takes a hydrogen atom from the tRNA, which is replaced with the methylthio group on MiaB. 

“Initially, the hydrogen on the tRNA is not positioned in a way that allows both access to the radical that removes it and access to the methylthio group that needs to be transferred, because the hydrogen and the atoms attached nearby are all aligned in the same plane,” said Booker. “Our structures show that the methylthio group on MiaB’s auxiliary cluster induces a change in geometry at that spot in the tRNA undergoing methylthiolation, which changes into more of a tetrahedral shape, with the hydrogen in an optimal position to be plucked off by the radical and the methylthio group in an optimal position for subsequent transfer.”

The result of these steps is tRNA with the added methylthio group and a successful modification.