A study on the process of mitochondrial biogenesis in apoptotic cells observed significant enhancement of mitochondrial biogenesis in the early process of radiation-induced apoptosis. The researchers from the Chinese Academy of Sciences published their results in iScience.
In this work, the team constructed a dual fluorescent reporter system, which can be used for real-time in situ observation of mitochondrial changes. The system was applied to the observation of apoptosis caused by ionizing radiation, and mitochondrial changes were determined by observing changes in the fluorescence ratio of dual fluorescent proteins.
"We detected cell viability, apoptosis, cell cycle, mitochondrial biogenesis, DNA damage repair proteins,” lead research Qing Huang said, explaining the detailed observation of cellular processes, "and carefully analyzed the process of mitochondrial dynamic changes.”
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They found that there was a significant enhancement of mitochondrial biogenesis in the early stage of radiation apoptotic cells. This result revised the previous generalized notion of negative correlation between them. This work not only provides an effective method to study mitochondrial biogenesis but also demonstrates how biological effects can be properly assessed at the right time points, highlighting the importance of the observation of dynamic processes in living cells.