Toll-1 Has Key Role in Cell Adhesion

According to researchers at Tohoku University, cell adhesion appears to be enabled by Toll-1, a transmembrane receptor protein that is better known for its role in the immune system.

"Many other theoretical works have emphasized the importance of differences in cell-to-cell adhesion for separating cell populations and maintaining the boundaries between them, but this had not yet been demonstrated in living animal epithelial tissues," says Erina Kuranaga, senior author on the paper published in Nature Communications recently. "Our study showed, for the first time, that cell sorting is regulated by changes in adhesion."

As fruit flies develop from the immature larval stage into the mature adult, histoblasts cluster together into several 'nests' in the abdomen. Each nest contains an anterior and a posterior compartment. Histoblasts are destined to replace larval cells to form the adult epidermis, the outermost layer that covers the flies. The cells in each compartment form discrete cell populations, so they need to stick together, with a distinct boundary forming between them.

Using fluorescent tags, Kuranaga and her team observed the Toll-1 protein is expressed mainly in the posterior compartment. Its fluorescence also showed a sharp boundary between the two compartments.

Further investigations showed Toll-1 performs the function of an adhesion molecule, encouraging similar cells to stick together. This process keeps the boundary between the two compartments straight, correcting distortions that arise as the cells divide to increase the number.

Interestingly, Toll proteins are best known for recognizing invading pathogens, and little is known about their work beyond the immune system. "Our work improves understanding of the non-immune roles of Toll proteins," says Kuranaga.