Beth Israel Deaconess Medical Center (BIDMC) scientists reported recently that antibiotic susceptibility assays, which are used in diagnostic laboratories to determine which antibiotics reliably treat which bacterial infections, might fall short and not be stringent enough.
To obtain consistently reliable results, researchers conducting antibiotic susceptibility assays follow national guidelines with standardized methods, including the inoculum that is added to each assay. There is a target inoculum, and then a range of an allowable inoculum, or acceptable upper and lower bounds around the target inoculum.
"Our question was whether this wiggle room impacts results," said co-author James Kirby, MD, director of the clinical microbiology laboratory at BIDMC. "Our findings were clear: inoculum matters." Kirby and his colleague Kenneth Smith, PhD published their findings in Antimicrobial Agents and Chemotherapy.
The investigators examined pathogens cited by the US Centers for Disease Control and Prevention and the World Health Organization as urgent and concerning drug resistance threats. "We found that the susceptibility determination against two big gun drugs—meropenem and cefepime—were dramatically affected by inoculum differences within the allowable range of inoculum," said Kirby. "Although we have no idea about how often clinical labs deviate even beyond the allowable range, we expect this happens with some frequency and would further skew results."
Kirby and Smith's findings indicate that clinical microbiology laboratories must hit the target inoculum pretty much on the nose to obtain reliable testing results for multidrug-resistant pathogens.