
Microarrays, also known as chips or biochips, consist of a substrate of interest fixed to a solid matrix such as a glass slide, used for genotyping, antibody detection and comparative genome analysis. Microarrays can be produced in house with specialized equipment, or purchased from a manufacturer. Microarray slides or chips are spotted with arrayers, hybridized in ovens, and refined by washers and strainers. Separate components can be purchased to produce, process, scan and analyze microarrays. Entire systems are available for scanning and analysis. A laboratory embarking on microarray experimentation should consider the option of self-produced vs. custom arrays. Arrays made “from scratch” provide a relatively low cost and easily customized microarray for each experiment. Commercially produced arrays have the benefit of reduced labor time and excellent reproducibility. In situ arrays require synthesis of the substrate directly onto the support. Budget, throughput, and available lab space are the important parameters for microarray equipment purchases.
Key differences between direct and indirect staining approaches
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The right choice can help maximize accuracy and efficiency.
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Upgrade your tissue culture room by getting accurate cell counts quickly, and recording results digitally. The Scepter™ 3.0 handheld cell counter combines smart capabilities with the Coulter impedance principal to hand you precise, consistent counts ...
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Measure and assess your cell cultures with ease. Our Millicell® DCI Digital Cell Imager enables more efficient execution of the repetitive daily techniques associated with cell passaging. Quickly and objectively assess confluency, morphology, ...
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The ImProm-II™ Reverse Transcription System from Promega offers a reliable way to achieve optimal results in cDNA synthesis.
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Microarray technology has been in use in the scientific community for the past few decades. This technique allows for obtaining data in an entire genome. Although enormous amounts of data can be obtained with a single experiment by this technique, ...
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