Our catalog lists protein ladders, standards, and markers from several suppliers for measuring protein molecular weights via electrophoresis. Methods for separating and detecting proteins, such as SDS-PAGE, native PAGE, 2-D electrophoresis, and Western blotting, require the loading of a ladder to determine the relative sizes of proteins. Commercial size markers are generally premixed and ready-to-use, and can come in a variety of options. Prestained protein ladders allow the user to continuously monitor the progression of electrophoresis. It can also let the user quickly assess the efficiency of blotting. Broad range markers contains proteins with low to high molecular weights, usually ranging between 10 to 240 kilodaltons. If the proteins of interest are known to be large or small, consider using high or low range markers, respectively.
Greater experimental consistency and improved throughput among the benefits
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Attention to detail is paramount to generation and analysis.
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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The use of a protein ladder is crucial for accurately determining protein sizes through SDS-PAGE analysis. In my experiments, I have relied on the Xpert 2 Prestained protein marker obtained from Gene Depot, which has consistently demonstrated ...
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As a reference, we use this native protein ladder for sizing estimation of proteins separated on native gels or for performing Western blots.
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