It is of considerable advantage to use high-quality gradient gels for protein separation by SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) especially when followed by western blotting. Gel percentage directly correlates to protein size and resolution, so choosing the correct gradient is the key to a well-resolved gel. Browse through our site listing page for polyacrylamide gels of various gradients, like 3-8%, 8-25%, and 4-12%. For labs with infrequent SDS-PAGE needs, Life Technologies™ provides a long shelf-life SDS-PAGE gradient gel, NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels, for the separation of 40-500kDa (kilo Dalton) proteins. In contrast, routine use shorter shelf-life 10-20% gradient gels are best used for proteins samples weighing between 12-130kDa. Important considerations for gradient gels are size, multiple well format and standard or high performance grade.
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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I highly recommend this 3-8 % gel for Immunoblotting of high molecular weight proteins. These Ready-to-use Precast Gels work very well and save a lot of time and energy.
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This product was great for western blot gels. The smaller wells only hold 30 µL; however, this is ample volume to run most protein westerns. This gel is ready to use and stores well at 4°C.
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