Running Buffers

Running buffers are necessary solutions for carrying out nucleic acid or protein electrophoretic separations, such as agarose gel electrophoresis and SDS-PAGE. Tris-acetate EDTA (TAE) and tris-borate EDTA (TBE) are two commonly used buffers in DNA gel electrophoresis. In polyacrylamide gel electrophoresis (PAGE) for protein separation, running buffers are commonly formulated with Tris-Glycine, MOPS, and MES. Buffers for running RNA gels can include MOPS and EDTA. Gel running buffers are often prepared in concentrated stock solutions, such as 10X or 20X, for convenience. Visit the supplier page to learn more about the buffer composition and intended applications.