Generation

Generating antibodies for therapeutic use is a complex process including immunization, hybridoma production, screening, and purification. Many of the technologies used in antibody production, including hybridoma technology, phage display, and B-cell isolation, have been relied on for years. Now, new methods, such as mRNA and ribosome display, transgenic mice, synthetic biology, and next-gen sequencing of B-cell repertoires, are being utilized to target challenging antigens as well as provide faster, more efficient, and more precise methods for identifying and optimizing candidate antibodies.