Biophysical methods are powerful tools for investigating protein structure, dynamics, and interactions. For therapeutic proteins, the key focus areas are aggregation, stability, and binding affinity. While no single method is perfect, combining multiple approaches yields comprehensive results. These accessible and cost-effective techniques provide invaluable insights into protein behavior, crucial for advancing therapeutic research and development. Want to learn more? Download our informative infographic now! This visual guide breaks down why biophysical methods are important, lists strengths and weaknesses, and provides application examples.