ATP (10 mM) from Cell Signaling Technology

Product Specs
ItemATP (10 mM)
CompanyCell Signaling Technology
Price $83.00 Inquire
Catalog Number9804S
Quantity1 ml
ModificationsATP (Adenosine triphosphate)
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A hotspot phosphorylation site on SHP2 drives oncoprotein activation and drug resistanceNature CommunicationsMarch 3, 2026Prashath Karunaraj, Remkes Scheele, Malcolm L. Wells, Ruchita Rathod, Ipek S. Gokulu, Sophia R. Abrahamson, Lila Taylor, Lamia Chowdhury, Abiha Kazmi, Weixiao Song, et al.the 10x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1 µg), the kinase of interest (0.2 µg) and ddH2O

(2) Targeting leucine-rich repeat kinase 2 overcomes resistance to oncolytic herpes simplex virus-based therapies in glioblastomaNature CommunicationsFebruary 28, 2026Yaning Qin, Zhiqi Liang, Mengqin Yu, Nanqian Lin, Naizhen Wang, Xuanke Huang, Shi Chen, Qun Peng, Yanhua Lin, Heqin Wang, et al.kinase buffer (Cell Signaling Technologies, 9802S) containing 200 µM ATP (Cell Signaling Technologies, 9804S) for 30 min at 30 °C. The reaction products were analyzed by Western blotting. RNA-seq analysis GBM

(3) AAK1 activation-mediated iron trafficking drives ferroptotic cell deathNature CommunicationsDecember 17, 2025Li-Chao Li, Zhi-Peng Ye, Ying Xiao, Xian-Ying Zhu, Qia-Qia Li, Yi-Qing Guo, Huai-Liang Wu, Zhi-Ling Li, Lin-Yu Wu, Yu-Hong Chen, et al.PKCβII kinase (Abcam, ab60841) in 1 × kinase buffer (CST, 9802S) in the addition of 400 μM ATP (CST, 9804S) with or without 0.5 μl λ-phosphatase at 30 °C for 1 h. Negative control was performed by the reaction

(4) Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progressionNature CommunicationsDecember 10, 2025Silu Sun, Bing Zhong, Ying Wang, Zaiye Li, Yuchen Jiang, Ning Ji, Xiaodong Feng, Rui Liu, Xin Zeng, Xikun Zhou, et al.resins were incubated with 1 μl of casein kinase II (New England Biolabs, P6010) and 200 μM ATP (CST, #9804) in the absence or presence of TBB with the indicated concentrations in 30 μl kinase buffer (25 mM Tris-HCl

(5) Targeting TNK2/ACK1 reverses the immunosuppressive tumor microenvironment and synergizes with immunochemotherapy in pancreatic cancerNature CommunicationsDecember 6, 2025Chao Wu, Weishuai Liu, Xiangting Hu, Yongjie Xie, Shengnan Li, Xinyue Liu, Zhaojun Sun, Xiaoling Li, Xin Yu, Yudong Yuan, et al.incubated with STAT5a, respectively in kinase reaction buffer (CST, #9802) containing 200 μM ATP (CST, #9804) on ice. After 30 min of incubation at 30 °C, SDS sample loading buffer was added to terminate the reactions.

(6) Lumpy skin disease virus LSDV087 positively regulates innate immune response by promoting oligomerization of MITA/STINGJournal of VirologyNovember 25, 2025Zhen-Zhen Li, Yu-Lin Yang, Meng-Yao Sun, Hong-Bing Shu, Li-Bo Cao(tlrl-pic, Invivogen), cGAMP ELISA Kit (CAY-501700-96S, Cayman), cGAMP (tlrl-nacga23-02, Invivogen), ATP (9804S, Cell Signaling Technology), GTP (51120, Sigma), digitonin (T2721, TargetMol), and aprotin, leupeptin,

(7) GSK461364 Inhibits NLRP3 Inflammasome by Targeting NEK7 PhosphorylationAdvanced ScienceNovember 1, 2025Ruiheng Luo, Mingliang Ma, Dan Wang, Ling Luo, Xueming Xu, Lingmin Huang, Fupeng Wang, Guangyan Kuang, Huiqi Liu, Rui Ni, et al.proteins, NEK7‐GST protein, were subjected to the kinase buffer(9802, CST) in the presence of 200 µm ATP(9804, CST)or without. The reaction mixtures were incubated for 60 min at 30 °C, stopped with 5x loading buffer,

(8) Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entryNatureOctober 2, 2025Debasish Paul, Derek L. Bolhuis, Hualong Yan, Sudipto Das, Xia Xu, Christina C. Abbate, Lisa M. M. Jenkins, Michael J. Emanuele, Thorkell Andresson, Jing Huang, et al.(Cell Signaling Technology, 9802). The assay was performed with 100 µM of ATP (Cell Signaling Technology, 9804) at 37 °C for 30 min, followed by addition of 4×-reducing SDS sample buffer and heating at 95 °C for

(9) Glycolytic Enzyme HK2 Phosphorylates nSMase1 to Promote Astrocytic Exosomes Biogenesis Contributing to Acute Ischemic Stroke InjuryAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Chen C, Zhou Y, Sheng L, Mai F, Ding Y, Hong S, Wu J, Pi J, Yan G, Huang Y, et al.lonidamine (100 µm) in 80 µL kinase reaction buffer (1 X Kinase Buffer (CST, 9802), 200 µm ATP (CST, 9804) in the present of protease/phosphatase inhibitor cocktail (TargetMol, C0001) at 37 °C for 1 h. Reactions

(10) Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug ResistanceAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Tian Q, Wang J, Li Q, Liu Y, Chen Y, Feng J, Lei ZN, Patel H, Cai CY, Xu Y, et al.inactive AKT WT/2A substrates were incubated in 1× kinase buffer (CST, 9802) with 200 µM ATP (CST, 9804) for 3 h at 4 °C. Phosphorylation was assessed by Western blot using p‐AKT (Ser473) and p‐AKT (Thr308)

(11) A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug ResistanceResearchSquareAugust 5, 2025Prashath Karunaraj, Prashath Karunaraj, Malcolm L. Scheele, Malcolm Rathod, Ipek Simay Rathod, Sophia Abrahamson, Sophia Abrahamson, Lamia Chowdhury, Lamia Chowdhury, Weixiao Kazmi, et al.the 5x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1 μg), the kinase of interest (0.2 μg) and ddH2O

(12) YTHDF3 recognizes DNA N6-methyladenine and recruits ALKBH1 for 6mA removal from genomic DNAThe EMBO JournalJuly 25, 2025Xin-Hui Chen, Zi-Lu Wang, Jincui Yang, Min Chen, Si-Yi Zhao, Kun-Xiong Guo, Xuelong Zheng, Zhengwei Zhao, Xiaoqiang Chen, Jing Li, et al.Diethyl pyrocarbonate,Sigma,Cat#V900882-10ML SYBR® Safe DNA gel stain,Invitrogen,Cat#S33102 Transfer RNAs (tRNA),Sigma,Cat#10109541001 ATP,CST,Cat#9804S HEPES,Genview,Cat#BH160 ascorbic acid,Sigma-Aldrich,Cat#A5960 (NH4)2Fe(SO4)2·6H2O,Sigma-Aldrich,Cat#203505 α-KG,Sigma-Aldrich,Cat#K1128 methylene

(13) A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug ResistancebioRxivJune 19, 2025Prashath Karunaraj, Remkes Scheele, Malcolm L. Wells, Ruchita Rathod, Sophia Abrahamson, Lila C. Taylor, Ipek S. Gokulu, Lamia Chowdhury, Abiha Kazmi, Weixiao Song, et al.the 5x Kinase buffer (Cell Signaling Technology, Cat# 9802S), ATP (Cell Signaling Technology, Cat# 9804S, final concentration 1 mM), SHP2 recombinant protein (1 μg), the kinase of interest (0.2 μg) and ddH2O

(14) RSK1-driven TRIM28/E2F1 feedback loop promotes castration-resistant prostate cancer progressionJ Clin InvestJune 16, 2025Miyeong Kim, Jinpeng Liu, Yanquan Zhang, Ruixin Wang, Ryan Goettl, Jennifer Grasso, Derek B. Allison, Chi Wang, Tianyan Gao, Xiaoqi Liu, et al.For Western blot, cold kinase assay was performed in which [γ -32P] ATP was replaced with normal ATP (#9804, Cell Signaling Technology). In vitro functional assays. Cell proliferation assays were carried out

(15) White spot syndrome virus immediate-early protein (wsv100) antagonizes the NF-κB pathway to inhibit innate immune response in shrimpPLoS PathogJune 12, 2025Bang Xiao, Fang Kang, Qianqian Li, Junming Pan, Yue Wang, Jianguo He, Chaozheng Limaintained at a constant amount and varying concentrations of His-Dorsal-FL protein with ATP (CST, cat. no. 9804S) were added into 10 × buffer (CST, cat. no. 9802S) to obtain a 1 × reaction system. In the control,

(16) Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1Nucleic Acids ResJune 6, 2025Josée Guirouilh-Barbat, Iman Litchy Boueya, Camille Gelot, Gaëlle Pennarun, Christine Granotier-Beckers, Elodie Dardillac, Wei Yu, Chloé Lescale, Emilie Rass, Olivier Ariste, et al.(Millipore 14-276) in the presence of 1× kinase buffer (Cell Signaling, 9802), 5 μM ATP (Cell Signaling, 9804), and 10 μCi ATP [γ-32P] (Perkin Elmer, BLU0021 100UC) at 30°C for 15 min. Reactions were stopped by

(17) Suppression of NRAS-mutant melanoma growth with NRAS-targeting Antisense Oligonucleotide treatment reveals therapeutically relevant kinase co-dependenciesCommun Med (Lond)June 5, 2025Valentin Feichtenschlager, Yixuan James Zheng, Tiange Qu, Dasha Hohlova, Ciara Callanan, Linan Chen, Christopher Chen, Wilson Ho, Albert Lee, Yeonjoo Hwang, et al.Cell Signaling Technology®, cat.no.: 9802), (2) 250 nM ATP (prepared daily with 1X KaB; Cell Signaling Technology® cat.no.: 9804), (3) 200 µg/ml 11-mer peptide (lyophilized stocks originally prepared as 1 mg/ml in 1X KaB, 5% DMSO),

(18) Simvastatin overcomes the pPCK1‐pLDHA‐SPRINGlac axis‐mediated ferroptosis and chemo‐immunotherapy resistance in AKT‐hyperactivated intrahepatic cholangiocarcinomaCancer CommunicationsMay 29, 2025Jinghan Zhu, Yixiao Xiong, Yuxin Zhang, Huifang Liang, Kun Cheng, Yuanxiang Lu, Guangzhen Cai, Yang Wu, Yunhui Fan, Xiaoping Chen, et al.Rhine‐Westphalia, Germany) was incubated with or without 1 µg His‐AKT and 100 µmol/L adenosine triphosphate (ATP) (#9804, Cell Signaling Technology) for 1 h. After washing with PBS, beads were incubated with Myc‐LDHAWT/T248A

(19) CD47-blocking antibody confers metabolic benefits against obesityCell Reports MedicineMay 20, 2025Yajuan Su, Jingyu Sun, Xiaobo Li, Feier Huang, Yunhui Kong, Zian Chen, Jingzhi Zhang, Duran Qin, Xiangyi Chen, Zhaoyue Wang, et al.Cell Signaling Technology Inc) containing 500 ng of synthetic inactive SAMS peptide (HY-P0136, MCE) and 500 μM ATP (9804, Cell Signaling Technology Inc) at 30°C for 30 minutes. The reaction was terminated by adding 100 μL

(20) TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasisElifeApril 23, 2025Qianqian Ju, Wenjing Sheng, Meichen Zhang, Jing Chen, Liucheng Wu, Xiaoyu Liu, Wentao Fang, Hui Shi, Cheng Sunkinase assay buffer (Cell Signaling Technology, #9802), in which 200 µM ATP (Cell Signaling Technology, #9804) was added. The reaction was performed at 30°C for 30 min. The reaction was terminated by adding 5×loading

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ATP (10 mM) from Cell Signaling Technology

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ATP (10 mM) from Cell Signaling Technology

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