dATP Solution from New England Biolabs

Product Specs
ItemdATP Solution
CompanyNew England Biolabs
Price $60.00 Inquire
Catalog NumberN0440S
Quantity25 µmol
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Pathogenic CGG expansions in oculopharyngodistal myopathy exhibit distinct characteristics of each causative gene on the flanking sequences as well as methylation statusGenome MedMarch 27, 2026Nobuyuki Eura, Satoru Noguchi, Megumu Ogawa, Kyuto Sonehara, Ai Yamanaka, Takashi Kurashige, Shinichiro Hayashi, Yukinori Okada, Kazuma Sugie, Ichizo Nishinowith a mixture of Cas9 ribonucleoprotein complexes, Taq polymerase (NEB, #M0273S), and dATP (NEB, #N0440S) at 37 °C for 30 min, followed by 72 °C for 5 min for Cas9 ribonucleoprotein complexes cleavage and

(2) Protocol for marker-free genome editing in Saccharomyces cerevisiae using universal donor templates and multiplexed CRISPR-Cas9STAR ProtocolsMarch 1, 2026Darshi Hemani, James H. Grissom, Richard J. ChiDihydrate,Sigma-Aldrich,L6883-1KG PEG-3350,VWR,0955-1KG Phusion High Fidelity PCR Kit,Thermo Fisher Scientific,F553L 100 mM dATP,New England Biolabs,N0440S 100 mM dCTP,New England Biolabs,N0441S 100 mM dGTP,New England Biolabs,N0442S 100 mM dTTP,New England

(3) Topologically Associating Domains Define the 3D Genome Architecture of Mouse Totipotent-like Stem CellsJournal of Biological ChemistryFebruary 5, 2026Yu Fu, Wenyu Ma, Jin Han, Wenhui Zhang, Shenghu Zhang, Yuxiao Chen, Yue Qin, Junyang Li, Ziwei Zhai, Pengli Li, et al.HpyCH4V (NEB, R0620L) at 37 °C overnight. A-tailing of the digestion ends was achieved by dATP (NEB, N0440S), Klenow Large fragment (3′ → 5′ exo-) (NEB, M0212L), and BSA (NEB, B9000S) for 1 h at 37 °C. The reaction

(4) DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand breakCell RepDecember 23, 2025Francesca Esposito, Ilaria Capozzo, Adelaide Riccardi, Ubaldo Gioia, Stefania Modafferi, Letizia Manfredi, Alessio Colantoni, Sara Tavella, Matteo Cabrini, Fabio Iannelli, et al.TCCCTAGCGTAACTCTCGAGGTAGTA-3′), 10 μL 10× T4 Ligase Buffer (NEB; #B0202S), 1 μL dATP solution 100 mM (NEB; #N0440S), 1 μL BSA (20 mg/mL; NEB; #B9200S) and 81 μL H2O. Coverslips were washed twice in PBS and processed

(5) Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interferenceNucleic Acids ResDecember 11, 2025Jiulin Chan, Zhichao Wu, Mingli Liu, Tianming Wang, Hanyu Liu, Ruimeng Cao, Xiaolong Li, Xinwen Li, Siyao Zhan, Jiangbo Cheng, et al.Klenow (3′-5′ exo-) solution [40 μl of NEBuffer™ 2 (NEB, Cat#B7002S), 8 μl of 10 mM dATP (NEB, Cat#N0440S), 40 μl of 10% (v/v) Triton X-100, 308 μl of H2O, and 4 μl of Klenow (NEB, Cat#M0212S)] at 37°C for

(6) Integration of Short- and Long-Read RNA Sequencing Enables the Discovery of Circular RNAsCancer ResearchDecember 2, 2025Sidi Z. Swift, Jace Webster, Hung Mai, Ghofran Othoum, Li Lin, Amy Ly, Emily B. Rozycki, Jacqueline L. Mudd, Emily Coonrod, Ashna Agarwal, et al.using PCR-based methods with the following components: Taq DNA Polymerase (NEB #M0273S), dNTPs (NEB #N0440S, #N0441S, #N0442S, #N0443S) and oligo primers from Integrated DNA Technologies (IDT) (Integrated DNA

(7) Integrating Hi-C data and RNA-seq data to reveal the 3D genome in sheepBMC GenomicsNovember 17, 2025Quanzhong Xu, Xiaoxue Zhang, Fadi Li, Huibin Tian, Deyin Zhang, Kai Huang, Yukun Zhang, Yuan Zhao, Xiaolong Li, Liming Zhao, et al.R0137L) at 37 °C for 7 h. Following digestion, the samples were incubated with dATP (NEB, Catalog No.: N0440S) at 37 °C for 1 h to add adenine to the ends of the digested chromatin fragments. Subsequently, the

(8) Library Preparation for Genome-Wide DNA Methylation ProfilingBIO-PROTOCOLNovember 5, 2025Fei-Man Hsu, Matteo Pellegrini, Pao-Yang Chen(New England Biolabs, catalog number: N0447S); store at -20 °C 13. dATP solution (New England Biolabs, catalog number: N0440S); store at -20 °C 14. Qubit dsDNA BR Quantification Assay kit (Invitrogen, catalog number: Q32850);

(9) Direct measurement of mavacamten and deoxyATP perturbation of the SRX/DRX ratio in porcine cardiac myofibrils using a simple, accessible and multiplexed approachJournal of Muscle Research and Cell MotilityOctober 22, 2025Matvey Pilagov, Ateeqa Naim, Kenneth S. Campbell, Thomas Kampourakis, Michael A. Geeves, Neil M. Kadfinal concentration of 30 µM with a final DMSO of 0.3% v/v. For 100% dATP experiments, 5 mM dATP (NEB N0440S) was used in the Chase buffer replacing unlabelled ATP. Data analysis To extract the rate of fluorescence

(10) The twist-and-squeeze activation of CARF-fused adenosine deaminase by cyclic oligoadenylatesThe EMBO JournalOctober 17, 2025Charlisa Whyms, Yu Zhao, Doreen Addo-Yobo, Huan He, Arthur Carl Whittington, Despoina Trasanidou, Carl Raymund P Salazar, Raymond H J Staals, Hong LiAdenosine 5’-diphosphate sodium salt,Sigma-Aldrich,A2754-100MG N6-Methyladenosine 5’-monophosphate sodium salt,Fisher Scientific,50-490-880 dATP solution,New England Biolabs,N0440S Adenosine 5’-Triphosphate (ATP),New England Biolabs,P0756S Quick CIP,New England Biolabs,M0525S rCutSmart Buffer

(11) Discovery of Natural Compound α‐Hederin via Large‐Scale Screening as a Targeted JAK/STAT3 Inhibitor for Ovarian Cancer TherapyAdvanced ScienceJuly 16, 2025Jiayu Wang, Pengzhan He, Cheng Liu, Xin Chen, Yilin Tan, Rui Qu, Yan Zhang, Zhou Li, Tailang Yin, Zhinang YinChem (Canada), were incubated with specified concentrations of α‐Hederin or ATP (New England Biolabs, N0440S) in a binding buffer (comprising 50 mM Tris pH 7.4, 150 mm NaCl, 1 mm EDTA, 6 mm sodium deoxycholate,

(12) Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformationNat CommunJuly 1, 2025Baohua Tan, Linjun Hong, Liyao Xiao, Jiajin Wu, Geyan Lu, Shanshan Wang, Langqing Liu, Enqin Zheng, Gengyuan Cai, Zicong Li, et al.R0137V) into blunt-end fragments. Cleaved chromatin was A-tailed by adding 10 mM dATP solution (NEB, N0440S) and Klenow Fragment (3′ → 5′ exo-) (NEB, M0212L) with rotation at 37 °C for 40 min. Then, chromatin

(13) Human ribomes reveal DNA-embedded ribonucleotides as a new type of epigenetic markbioRxivJuly 1, 2025Deepali Lalchand Kundnani, Taehwan Yang, Tejasvi Channagiri, Penghao Xu, Yeunsoo Lee, Mo Sun, Francisco Martinez-Figueroa, Supreet Randhawa, Ashlesha Gogate, Youngkyu Jeon, et al.Kit (Qiagen catalog no. 28104). The fragments were tailed with dATP (New England Biolabs catalog no. N0440S) by using Klenow Fragment (3′→5′ exo-) (New England Biolabs catalog no. M0212L) for 30 min at 37 °C

(14) Single-cell parallel analysis of DNA damage and transcriptome reveals selective genome vulnerabilityNature MethodsMay 1, 2025Dongsheng Bai, Zhenkun Cao, Nivedita Attada, Jinghui Song, Chenxu Zhuβ-Nicotinamide adenine dinucleotide (NAD+, NEB, B9007S), 4 μL mixed dNTPs (5 μM for each dATP (NEB, N0440S), dCTP (NEB, N0441S), dGTP (NEB, N0442S), biotin-dUTP (Thermo Scientific, R0081)) were added into nuclei

(15) Improved cohesin HiChIP protocol and bioinformatic analysis for robust detection of chromatin loops and stripesCommun BiolMarch 14, 2025Karolina Buka, Zofia Parteka-Tojek, Abhishek Agarwal, Michał Denkiewicz, Sevastianos Korsak, Mateusz Chiliński, Krzysztof H. Banecki, Dariusz Plewczynskimaster mix was added: 67.5 μl of water, 4.5 μl of 10 mM dTTP (NEB, N0443S), 4.5 μl of 10 mM dATP (NEB, N0440S), 4.5 μl of 10 mM dGTP (NEB, N0442S), 45 μl of 1 mM biotin-16-dCTP (Jena Bioscience NU-809-BIO16-S),

(16) Cancer-independent somatic mutation of the wild-type NF1 allele in normal tissues in neurofibromatosis type 1Nature GeneticsFebruary 25, 2025Thomas R. W. Oliver, Andrew R. J. Lawson, Henry Lee-Six, Anna Tollit, Hyunchul Jung, Yvette Hooks, Rashesh Sanghvi, Matthew D. Young, Timothy M. Butler, Pantelis A. Nicola, et al.T4 DNA ligase buffer (New England Biolabs, B0202S), 1.5 μl (1 mM) dATP/ddBTP (New England Biolabs, N0440S; GE HealthCare, 27204501), 1.5 μl Klenow fragment (3′–5′ exo-; New England Biolabs, M0212L) and 0.5

(17) Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencingNucleic Acids ResJanuary 16, 2025Sagnik Sen, Pierre-Olivier Estève, Karthikeyan Raman, Julie Beaulieu, Hang Gyeong Chin, George R Feehery, Udayakumar S Vishnu, Shuang-Yong Xu, James C Samuelson, Sriharsa Pradhanof genomic DNA was incubated with 30 U of T4 DNA Polymerase (NEB # M0203L), 25 μM dATP/dGTP (NEB # N0440S/N0442S, respectively) in 1X NEBufferTM2 for 4 h at 20°C. Enzyme was inactivated for 20 min at 75°C.

(18) Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factorbioRxivJanuary 12, 2025Zoe L. Grant, Shuzhen Kuang, Shu Zhang, Abraham J. Horrillo, Kavitha S. Rao, Vasumathi Kameswaran, Carine Joubran, Pik Ki Lau, Keyi Dong, Bing Yang, et al.purification. Entire eluate was used for A-tailing in a 50 μl reaction with 1 mM dATP (New England Biolabs #N0440S), 15 U Klenow 3’>5’ exonuclease (New England Biolabs #M0212L) and 1x NEB buffer 2 in nuclease-free water

(19) Comprehensive multi‐tissue epigenome atlas in sheep: A resource for complex traits, domestication, and breedingiMetaDecember 15, 2024Deyin Zhang, Jiangbo Cheng, Xiaolong Li, Kai Huang, Lvfeng Yuan, Yuan Zhao, Dan Xu, Yukun Zhang, Liming Zhao, Xiaobin Yang, et al.was digested with AluI endonuclease (R0137L; NEB) at 37°C for 7 h, followed by the addition of dATP (N0440s; NEB) and incubation at 37°C for 1 h. DNA ligation was performed using T4 DNA ligase containing a biotin

(20) The CRISPR-associated adenosine deaminase Cad1 converts ATP to ITP to provide antiviral immunityCellDecember 12, 2024Christian F. Baca, Puja Majumder, James H. Hickling, Linzhi Ye, Marianna Teplova, Sean F. Brady, Dinshaw J. Patel, Luciano A. Marraffinial.29,N/A PhiJ4,Banh et al.29,N/A "Chemicals, peptides, and recombinant proteins",, ATP,NEB,P0756S dATP,NEB,N0440S ITP,Sigma Aldrich,I0879–50MG dITP,Thermo Fisher,R1191 Adenosine,Sigma Aldrich,A9251–1G Inosine,Sigma Aldrich

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dATP Solution

dATP Solution from New England Biolabs

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dATP Solution from New England Biolabs

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