Human Total IL-18 DuoSet ELISA DY318-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Total IL-18 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price Supplier Page View Company Product Page
Catalog NumberDY318-05
Quantity1 Kit (for 5 Plates)
ApplicationsELISA
Detection Range11.70 - 750 pg/mL
ReactivityHuman
Detection TargetIL-18/IL-1F4
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterleukin 18
NCBI Gene AliasesIGIF, IL-18, IL-1g, IL1F4

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Putative α7-selective ligands interact with α9-containing nicotinic acetylcholine receptors and modulate immune functions of human mononuclear phagocytesFront ImmunolMarch 11, 2026Mona Mobasher, Arik J. Hone, Malin Pilzecker, Daria Bozic, Andreas Hecker, J. Michael McIntosh, Karl N. Kirschner, Veronika Grau, Roger L. Papke, Hina Andleeb, et al.enzyme-linked immunosorbent assay (ELISA) from R&D Systems (Cat# DY201) and human Total IL-18 DuoSet ELISA (Cat# DY318-05) according to the supplier’s instructions. Bioactive IL-1α/β in the cell-free supernatants was quantified

(2) The gut microbiota composition is shaped by disease activity and individual treatment responses in patients with multiple sclerosisFrontiers in ImmunologyFebruary 12, 2026Veronika Ticha, Stepan Coufal, Zuzana Jiraskova Zakostelska, Tomas Thon, Radka Roubalova, Tomas Hrncir, Miloslav Kverka, Miluse Pavelcova, Pavlina Kleinova, Jana Lizrova Preiningerova, et al.,R&D systems,DY383 S100A8/S100A9 heterodimer,S100A8/S100A9; calprotectin,R&D systems,DY8226-05 Interleukin-18,IL-18,R&D systems,DY318-05 Lipocalin-2/NGAL,Lipocalin,R&D systems,DY1757 Serum Amyloid A,SAA,R&D systems,DY3019-05 Human Short-Chain Fatty Acids

(3) RSV temporally reprograms apoptosis and pyroptosis to balance immune evasion and replicationScience AdvancesJanuary 23, 2026Cong Liu, Haiwu Zhou, Jian Li, Yang Meng, Jingyu Wang, Mingbin He, Weiwei Wang, Zhifei Li, Yali Qin, Mingzhou Chen(catalog no. 430204) ELISA kits were purchased from BioLegend, and human IL-18 ELISA kit (catalog no. DY318-05) was purchased from R&D Systems. ASC oligomerization and speck formation assay The PMA-differentiated

(4) Ongoing lymphoid HIV production drives pyroptosis and GLP-1 counter-regulation in ART-suppressed infectionbioRxivJanuary 14, 2026Peter A. Crawford, Joshua Rhein, Jeffrey G. Chipman, Gregory J. Beilman, Ross Cromarty, Kevin Escandón, Jodi Anderson, Garritt Wieking, Andrew Johnston, Afeefa Ahmed, et al.IL-23, TNF-α, IFN-γ, and MIP-1β) Luminex panel (Cat. HSTCMAG-28SK, MILLIPLEX) and by ELISA (IL-18) (Cat. DY318–05, R&D systems). All processing was performed according to the kit manufacturer’s instructions. Values

(5) NLRP3 Inflammasome and Polycystic Ovary Syndrome (PCOS): A Novel Profile in Adipose TissueInternational Journal of Molecular SciencesJanuary 9, 2026Salih Atalah Alenezi, Khalid Alshammari, Raheela Khan, Saad Amerwere measured using DuoSet ELISA kits from R&D Systems (Minneapolis, MN, USA) (IL-1β: DY201; IL-18: DY31805), following the manufacturer’s recommended protocol. 96-well microplates were coated overnight at room

(6) Cholesterol-mediated inflammation activation in alveolar macrophagesBMC BiolDecember 22, 2025Sonia Giambelluca, Matthias Ochs, Elena Lopez-RodriguezIL-1beta/IL-1F2, DY201; Mouse IL-1beta/IL-1F2, DY401-05, R&D System, MN, USA) and IL-18 (Human Total IL-18, DY318-05; Mouse IL-18, DY7625-05, R&D System, MN, USA) in undiluted cell media, according to the manufacturer’s

(7) Ocrelizumab transiently alters microbiota and modulates immune response depending on treatment outcomeiScienceDecember 19, 2025Stepan Coufal, Zuzana Jiraskova Zakostelska, Tomas Thon, Radka Roubalova, Dominika Kadleckova, Martina Salakova, Ruth Tachezy, Tomas Hrncir, Miloslav Kverka, Veronika Ticha, et al.peptides, and recombinant proteins" ,, CCL17/TARC,R&D systems,Cat#DY364 Interleukin-18,R&D systems,Cat#DY318-05 Mannose-Binding Lectin,R&D systems,Cat#DY2307 Lipopolysaccharide-Binding Protein,R&D systems,Cat#DY870 Soluble

(8) Skin and gut microbiota composition and immune regulatory response differentiate IgE and non-IgE cow’s milk allergy patients with atopic dermatitisiScienceDecember 19, 2025Tomas Thon, Eliska Kopelentova, Dagmar Srutkova, Stepan Coufal, Jakub Kreisinger, Filip Rob, Zuzana Reiss, Miloslav Kverka, Stepanka Capkova, Jana Cadova, et al.,R&D systems,Cat#DY3078 "L-FABP, Human, ELISA kit",Hycult Biotech,Cat#HK404-02 Human Total IL-18 DuoSet ELISA,R&D systems,Cat#DY318-05 Human CD14 DuoSet ELISA,R&D systems,Cat#DY383 Human CCL11/Eotaxin DuoSet ELISA,R&D systems,Cat#DY320 Human IgE Uncoated ELISA Kit

(9) BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4+ T cellsCell Reports MedicineSeptember 16, 2025Hongbo Gao, Yuhao Li, Ritudwhaj Tiwari, Marilia Pinzone, Xiwen Qin, Kolin M. Clark, Sara K. Nicholson, Tony Yao, Kelly Rome, Michael Scaglione, et al.Human IFN-alpha 2 Quantikine HS ELISA Kit,R&D Systems,HSIFNA2 Human Total IL-18 DuoSet ELISA,R&D Systems,DY318-05 P3 Primary Cell 4D-Nucleofector X Kit,Lonza,V4XP-3032 Quick-RNA Viral Kit,Zymo Research,R1035 Quick-RNA Microprep

(10) Inflammasome-independent IL-1β activation via staphopain A protease of Staphylococcus aureusJournal of Biological ChemistryAugust 8, 2025Stefan Bauernfried, Tobias Komar, Katja Sterle, Maria C. Tanzer, Alexander R. Horswill, Matthias Mann, Veit HornungBD Biosciences). hIL-6 ELISAs (catalog no.: 555220, BD Biosciences) and hIL-18 ELISAs (catalog no.: DY318-05, R&D Systems) were performed according to the supplier’s protocol. Immunoblotting Whole-cell lysates

(11) Modulation of IRAK4 as a Therapeutic Strategy Against Monosodium Urate- and Xanthine-Induced Inflammation in Macrophages and HepG2 CellsResearch squareJuly 16, 2025Umar S, Chang HT, Maienschein-Cline M, Ravindran S.

(12) NLRP11 is required for canonical NLRP3 and non-canonical inflammasome activation during human macrophage infection with mycobacteriamBioMay 14, 2025Mateusz Szczerba, Akshaya Ganesh, María Luisa Gil-Marqués, Volker Briken, Marcia B. Goldbergwere assayed for levels of secreted cytokines using Human Total IL-18 DuoSet ELISA kit (Bio-Techne, DY318-05) and ELISA MAX Deluxe Set Human IL-1β kit (BioLegend, 437004) following the manufacturers’ instructions. Protein extraction

(13) Acidosis Licenses the NLRP3 Inflammasome-Inhibiting Effects of Beta-Hydroxybutyrate and Short-Chain Carboxylic AcidsbioRxiv : the preprint server for biologyMay 7, 2025Mank MM, Zoller KA, Fastiggi VA, Ather JL, Poynter ME.

(14) Cytokine profiles dynamics in COVID-19 patients: a longitudinal analysis of disease severity and outcomesScientific reportsApril 23, 2025Ghaffarpour S, Ghazanfari T, Ardestani SK, Naghizadeh MM, Vaez Mahdavi MR, Salehi M, Majd AMM, Rashidi A, Chenary MR, Mostafazadeh A, et al.

(15) Treatment of IL-18-binding protein biologics suppresses fibrotic progression in metabolic dysfunction-associated steatohepatitisCell Reports MedicineApril 15, 2025Dong-Hyun Kim, Gona Choi, Eun-Bi Song, Hanna Lee, Jaehui Kim, Young-Saeng Jang, JinJoo Park, Susan Chi, Jaekyu Han, Sun-Mi Kim, et al.commercial assays,Critical commercial assays ,, Human Total IL-18 DuoSet ELISA Kit,R&D systems,Cat# DY318-05 RNeasy mini kit,Qiagen,Cat# 47106 RNeasy Micro kit,Qiagen,Cat# 74004 High-capacity cDNA reverse transcription

(16) Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activationEMBO Molecular MedicineFebruary 13, 2025Léana Gorse, Loïc Plessis, Stephen Wearne, Margaux Paradis, Miriam Pinilla, Rae Chua, Seong Soo Lim, Elena Pelluz, Gee-Ann TOH, Raoul Mazars, et al.Antibody Array,Abcam,ab134003 IL-1 β Human Uncoated ELISA Kit,Invitrogen,88-7261-77 Human Total IL-18 DuoSet ELISA,Bio-Techne,DY318-05 Human IL-1α Platinum ELISA Kit,Invitrogen,BMS243-2 Cyquant LDH,Invitrogen,C20301 "MACSxpress Whole

(17) ECDD-S16, a synthetic derivative of cleistanthin A, suppresses pyroptosis in Burkholderia pseudomallei-infected U937 macrophagesPloS oneJanuary 1, 2025Khongpraphan S, Sanongkiet S, Luangjindarat C, Thairat S, Munyoo B, Chabang N, Charoensutthivarakul S, Borwornpinyo S, Tuchinda P, Ponpuak M, et al.

(18) N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formationCytojournalDecember 20, 2024Le Wang, Jinjing Huang, Lei Song, Ben KeS0057S; China). The release of interleukin-1b (IL-1b) (R&D Systems; PMLB00C; USA) and IL-18 (R&D Systems; DY318-05; USA) was also assessed by ELISA, following the manufacturer’s protocols. Western blot Total protein

(19) Decoy-resistant IL-18 reshapes the tumor microenvironment and enhances rejection by anti-CTLA-4 in renal cell carcinomaJCI insightNovember 19, 2024Schoenfeld DA, Djureinovic D, Su DG, Zhang L, Lu BY, Kamga L, Mann JE, Huck JD, Hurwitz M, Braun DA, et al.artifacts. ELISA. IL-18 and IL-18BP ELISAs were performed using the Human Total IL-18 DuoSet ELISA (R&D Systems, DY318-05), DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems, DY008B), and human IL-18BP ELISA Kit (Abcam, ab100559)

(20) The DNA sensor AIM2 mediates psoriasiform inflammation by inducing type 3 immunityJCI InsightNovember 8, 2024Timna Varela Martins, Bruno Marcel Silva de Melo, Juliana Escher Toller-Kawahisa, Gabriel Victor Lucena da Silva, Conceição Elidianne Aníbal Silva, Isadora Marques Paiva, Gabriel Azevedo Públio, Marcos Henrique Rosa, Cacilda da Silva Souza, Dario Simões Zamboni, et al.protocols using the DuoSet ELISA kit (IL-1 β, DY401; IL-17, DY421; IL-23, DY1887; IL-6, DY406; IL-18, DY318-05; IL-8/CXCL-8, DY208; CXCL1/KC, DY208; R&D Systems). KC culture in vitro. Human KCs cell line (HaCaT)

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Human Total IL-18 DuoSet ELISA

Human Total IL-18 DuoSet ELISA

Human Total IL-18 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human Total IL-18 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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