Human NT-4 DuoSet ELISA DY268 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman NT-4 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY268
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range31.20 - 2000 pg/mL
ReactivityHuman
Detection TargetNT-4
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameneurotrophin 4
NCBI Gene AliasesGLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discoveryScientific ReportsFebruary 27, 2026Emilia Sinderewicz, Maria Dąbkowska, Anna Sarnowska, Joanna Staszkiewicz-Chodor, Dorota Mystkowska, Piotr Holak, Igor Drozd, Magdalena Chodkowska-Michalowska, Monika Rytel, Edyta Paczkowska, et al.available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept constant at 298 ± 0.1 K. PEG Poly(ethylene

(2) The bacterial metabolite, lithocholic acid, has antineoplastic effects in pancreatic adenocarcinomaCell death discoveryMay 23, 2024Schwarcz S, Kovács P, Nyerges P, Ujlaki G, Sipos A, Uray K, Bai P, Mikó E.Sigma-Aldrich) was used at a concentration of 500 U/ml. BA receptor antagonists (NF449 [95], CINPA1 [96], DY268 [97], and GSK2033 [98]) were acquired from Tocris Bioscience (Bristol, UK), and ketoconazole [99] was

(3) Altered microbial bile acid metabolism exacerbates T cell-driven inflammation during graft-versus-host diseaseNature microbiologyMarch 1, 2024Lindner S, Miltiadous O, Ramos RJF, Paredes J, Kousa AI, Dai A, Fei T, Lauder E, Frame J, Waters NR, et al.Scientific) in 50uL of DMEM with 10% FBS (Gibco). After an overnight incubation, cells were exposed to BA or DY268 (Tocris, 20nM) in 50μL for another 22–24h. Viability was determined using Hoechst 33342 (Invitrogen).

(4) Altenusin, a fungal metabolite, alleviates TGF-β1-induced EMT in renal proximal tubular cells and renal fibrosis in unilateral ureteral obstructionHeliyonFebruary 15, 2024Natechanok Thipboonchoo, Somsak Fongsupa, Sanya Sureram, Suliporn Sa-nguansak, Chatchai Kesornpun, Prasat Kittakoop, Sunhapas Soodvilaiantibodies from Abcam (MA, USA). The GW4064 (G5172) was purchased from Sigma Aldrich (DE, USA.), and DY268 (5656) was obtained from Tocris Bioscience (MN, USA). All other chemicals were analytical grade and

(5) The Impact of Serum Protein Adsorption on PEGylated NT3-BDNF Nanoparticles-Distribution, Protein Release, and Cytotoxicity in a Human Retinal Pigmented Epithelial Cell ModelPharmaceuticsAugust 30, 2023Dąbkowska M, Kosiorowska A, Machaliński B.available enzyme-linked immunosorbent assay (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept at a constant value equal to 298 ± 0.1 K. 2.1.2.

(6) Selective PPARδ agonist seladelpar suppresses bile acid synthesis by reducing hepatocyte CYP7A1 via the fibroblast growth factor 21 signaling pathwayThe Journal of biological chemistryJuly 1, 2022Kouno T, Liu X, Zhao H, Kisseleva T, Cable EE, Schnabl B.Therapeutics. Wy14643 (PPARα agonist) and GW4064 (FXR agonist) were purchased from MilliporeSigma. DY268 (FXR antagonist) was from Axon Medchem. PPARG antagonist GW9662 was from Tocris Bioscience. FGF21 recombinant

(7) Proglumide Reverses Nonalcoholic Steatohepatitis by Interaction with the Farnesoid X Receptor and Altering the MicrobiomeInternational journal of molecular sciencesFebruary 8, 2022Gay MD, Cao H, Shivapurkar N, Dakshanamurthy S, Kallakury B, Tucker RD, Kwagyan J, Smith JP.the reference agonist, and then dispensed in 100 µL in all wells. Next, 100 µL of 2× concentration of DY268, (Tocris, Bristol, UK, Cat # 1609564-75-1) the antagonist, was dispensed in wells designated with the

(8) The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro settingJournal of NanobiotechnologyDecember 1, 2021Maria Dąbkowska, Zofia Ulańczyk, Karolina Łuczkowska, Dorota Rogińska, Anna Sobuś, Monika Wasilewska, Maria Olszewska, Katarzyna Jakubowska, Bogusław Machalińskiwere conducted by solution depletion ELISA technique (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). Afterward, to determine unbound BDNF molecules more accurately after adsorption at PAMAM

(9) DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse ModelFrontiers in endocrinologyJanuary 1, 2021Tao J, Yu XL, Yuan YJ, Shen X, Liu J, Gu PP, Wang Z, Ma YT, Li GQ.or DMRT2 overexpression vector was co-transfected in 3T3-L1 adipocytes in the presence or absence of DY268 (5 μM for 24 h, R&D Systems). Forty-eight hours after transfection, luciferase alterations were determined

(10) Novel design of (PEG-ylated)PAMAM-based nanoparticles for sustained delivery of BDNF to neurotoxin-injured differentiated neuroblastoma cellsJournal of NanobiotechnologyDecember 1, 2020Maria Dąbkowska, Karolina Łuczkowska, Dorota Rogińska, Anna Sobuś, Monika Wasilewska, Zofia Ulańczyk, Bogusław Machalińskiavailable Enzyme-Linked Immunosorbent Assay (ELISA) (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of experiments was kept at a constant value equal to 298 ± 0.1 K. PAMAM The

(11) OATP1B3-1B7, a novel organic anion transporting polypeptide, is modulated by FXR ligands and transports bile acidsAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyDecember 1, 2019Vanessa Malagnino, Janine Hussner, Ali Issa, Angela Midzic, Henriette E. Meyer zu Schwabedissenthree individuals (BioChain, Newark, CA). Cells were treated with 100 ␮M CDCA, 10 ␮M GW4064 (32), 1 ␮M DY268 (Tocris, Bristol, UK) (31), or DMSO 24 h (Huh-7) or 4 h (HepaRG) after seeding. RNA was isolated after

(12) Lithocholic Acid, a Metabolite of the Microbiome, Increases Oxidative Stress in Breast CancerCancersAugust 27, 2019Kovács P, Csonka T, Kovács T, Sári Z, Ujlaki G, Sipos A, Karányi Z, Szeőcs D, Hegedűs C, Uray K, et al.Gsα-selective antagonist). To inhibit nuclear receptor activation CINPA1 (CAR receptor antagonist), DY268 (FXR receptor antagonist), GSK2033 (LXR receptor antagonist)) were used. RA839, an NRF2 activator, which

(13) Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivoInternational journal of nanomedicineJanuary 1, 2019Dąbkowska M, Rogińska D, Kłos P, Sobuś A, Adamczak M, Litwińska Z, Machalińska A, Machaliński B.determined by commercially available ELISA kits (DY992, DY990, DY994, DY999, DY995, WA126, DY006, and DY268; R&D Systems). The temperature of experiments was kept at a constant 298±0.1 K. A suspension of PAMAM

(14) Cholestatic Drug Induced Liver Injury: A Function of Bile Salt Export Pump Inhibition and Farnesoid X Receptor AntagonismApplied In Vitro ToxicologySeptember 1, 2018Jonathan P. Jackson, Kimberly M. Freeman, Robert L. St. Claire, Chris B. Black, Kenneth R. BrouwerArbor, MI). CsA and chenodeoxycholic acid (CDCA) were purchased from Sigma Aldrich (St. Louis, MO). DY268 was purchased from Tocris (Bristol, United Kingdom). Deuterated-TCA (d8-TCA) was purchased from Martrex

(15) Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic strokeScientific reportsFebruary 1, 2018Kim YR, Ahn SM, Pak ME, Lee HJ, Jung DH, Shin YI, Shin HK, Choi BT.BDNF ELISA kit (Cat. DBD00, R&D Systems Inc., Minneapolis, MN, USA), an NT-4 DuoSet ELISA kit (Cat. DY268, R&D Systems Inc.), and a Quantikine VEGF ELISA kit (Cat. MMV00, R&D Systems Inc.). Final reactions

(16) Serum levels of neurotrophic factors in active toxoplasmic retinochoroiditisThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesMarch 1, 2017Cordeiro CA, Vieira EL, Rocha NP, Castro VM, Oréfice JL, Barichello T, Costa RA, Oréfice F, Young L, Teixeira AL.

(17) Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunityBrain : a journal of neurologyAugust 1, 2010Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, et al.

(18) Neurotrophins modulate monocyte chemotaxis without affecting macrophage functionClinical and experimental immunologyMarch 1, 2008Samah B, Porcheray F, Gras G.

(19) Regulation of preimplantation embryo development by brain-derived neurotrophic factorDevelopmental biologyNovember 1, 2007Kawamura K, Kawamura N, Fukuda J, Kumagai J, Hsueh AJ, Tanaka T.

(20) Involvement of neurotrophins and their receptors in spondyloarthritis synovitis: relation to inflammation and response to treatmentAnnals of the rheumatic diseasesNovember 1, 2005Rihl M, Kruithof E, Barthel C, De Keyser F, Veys EM, Zeidler H, Yu DT, Kuipers JG, Baeten D.UK). Concentrations of the neurotrophic factors NGF (catalogue No DY256), BDNF (DBD00), NT-3 (DY267), and NT-4 (DY268) were measured in the synovial fluid samples of 15 patients with SpA, 15 with RA, and 10 patients with

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Human NT-4 DuoSet ELISA

Human NT-4 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human NT-4 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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