P450-Glo CYP2C9 Assay V8792 from Promega

Product Specs
ItemP450-Glo CYP2C9 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8792
Detection TargetCYP2C9
Sample TypeRecombinant and native protein, microsomal fractions
MethodLuminescent
Quantity50ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Distinct Roles of Matrigel Enabled the Production of Expandable Hepatoblast and Polarized Hepatocyte Organoids from Human Embryonic Stem Cells under 3-Dimensional Suspension ConditionsBiomaterials ResearchNovember 7, 2025Haibin Wu, Jue Wang, Shoupei Liu, Yiyu Wang, Jinghe Xie, Xueyan Zhang, Shuai Zhang, Weili Gu, Yongjian Zhou, Yuyou Duanh; then, CYP enzyme activities were measured by the P450-Glo Assay Kit (Promega, V9002, V8422, and V8791) according to the manufacturer’s instructions. Cell proliferation and fold-increase calculation For

(2) Liver portal fibroblasts induce the functions of primary human hepatocytes in vitroCommunications biologyMay 9, 2025Brown GE, Bodke VV, Ware BR, Khetani SR.incubating the cultures with substrates (luciferin-IPA for CYP3A4, V9002, and luciferin-H for CYP2C9, V8792, from Promega Life Sciences, Madison, WI) for 1 h and 3 h, respectively, at 37 °C and then detecting

(3) Loss of heterozygosity of CYP2D6 enhances the sensitivity of hepatocellular carcinomas to talazoparibeBioMedicineNovember 1, 2024Xiaonan Zhang, Natallia Rameika, Lei Zhong, Verónica Rendo, Margus Veanes, Snehangshu Kundu, Sandro Nuciforo, Jordan Dupuis, Muhammad Al Azhar, Ioanna Tsiara, et al.activity of different CYPs was detected following the protocol of P450-Glo™ Assay Kits of Promega (#V8421, V8791, V9001). Briefly, HepG2 cells (#HB-8065) obtained from ATCC and HepG2-CYP cells (HepG2-CYP1A2, HepG2-CYP2C9,

(4) Comparison of Engineered Liver 3D Models and the Role of Oxygenation for Patient‐Derived Tumor Cells and Immortalized Cell Lines Cocultured with Tumor Stroma in the Detection of HepatotoxinsAdvanced BiologyFebruary 1, 2024Mona Mansouri, Astha Lamichhane, Dola Das, Federico Aucejo, Hossein Tavana, Nic D. LeipzigCYP3A4-IPA (dilution: 1:1000, cat# V9001) and 6′deoxyluciferin (Luciferin H) (dilution: 1:50, cat# V8791, Promega) were used as substrates for CYP3A4 and CYP2C9, respectively. CYP3A4 and 2C9 levels were normalized

(5) Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities†Biomaterials ScienceNovember 21, 2023Ruben M. F. Tomás, Robert Dallman, Thomas R. Congdon, Matthew I. Gibson(CHE3158) were purchased from Scientific Laboratory Supplies, Nottingham, UK. P450-Glo™ CYP2C9 Assay (V8792) and P450-Glo™ CYP3A4 Assay (V9002) were purchased from Promega, Wisconsin, USA. Sambucus nigra (“Elder”)

(6) Label-free chemical imaging of cytochrome P450 activity by Raman microscopyCommunications biologyAugust 22, 2022Li M, Nawa Y, Ishida S, Kanda Y, Fujita S, Fujita K.density were taken as controls. CYP activity measurement CYP1A2 (V8422), CYP2B6 (V8322), CYP2C9 (V8792), and CYP3A4 (V9002) activity were measured using P450-GloTM assay purchased from Promega. The measurement

(7) Direct conversion of porcine primary fibroblasts into hepatocyte-like cellsScientific ReportsDecember 1, 2021Mariane Fráguas-Eggenschwiler, Reto Eggenschwiler, Jenny-Helena Söllner, Leon Cortnumme, Florian W. R. Vondran, Tobias Cantz, Michael Ott, Heiner Niemann37 °C, respectively. CYP1A2 was analysed using kit #V8422 and, CYP2C33 was analysed using the kit #V8792, for CYP2C9 (Promega). For internal control of CYP induction specificity, in all analyzed conditions

(8) Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived HepatocytesCellsMay 21, 2021Zahmatkesh E, Ghanian MH, Zarkesh I, Farzaneh Z, Halvaei M, Heydari Z, Moeinvaziri F, Othman A, Ruoß M, Piryaei A, et al.-1A2 in both groups, lytic assays were performed by using a P450-GloTM assay kit (V8802, V8322, and V8792, respectively; Promega) based on the manufacturer’s instructions. The LOs and BLOs derived from hESCs

(9) Differential Monocyte Actuation in a Three‐Organ Functional Innate Immune System‐on‐a‐ChipAdvanced ScienceJuly 1, 2020Trevor Sasserath et al.analysis. Similar methods followed for the 2C9 specific substrate, Luciferin‐H (400 × 10−6 m) (Promega, V8792), for which incubation time was also 3 h, and the 3A4 specific substrate, Luciferin‐IPA (12 × 10−6 m)

(10) FABP1 controls hepatic transport and biotransformation of Δ9-THCScientific ReportsDecember 1, 2019Matthew W. Elmes et al.was additionally assessed by luciferin-H substrate conversion using the CYP450-Glo CYP2C9 assay kit (#V8791; Promega, Madison, WI, USA) with 20 µg liver microsomes or BSA control, according to the manufacturer’s

(11) Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial CellsScientific reportsJune 20, 2019Pettinato G, Lehoux S, Ramanathan R, Salem MM, He LX, Muse O, Flaumenhaft R, Thompson MT, Rouse EA, Cummings RD, et al.Madison, WI), CYP2B6 (P450-Glo CYP2B6 – V8322 – Promega, Madison, WI), and the CYP2C9 (P450-Glo CYP2C9 – V8792 – Promega, Madison, WI) by incubating them with different inducers. For the CYP1A1 and CYP1A2 activity

(12) Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C VirusCurrent protocols in stem cell biologyAugust 14, 2017Sa-Ngiamsuntorn K, Hongeng S, Wongkajornsilp A.O104)Phenobarbital (Sigma, cat. no. P5178)EthanolP450‐Glo 1A1, 2B6, 2C9 and 3A4 assay kits (V8751, V8321, V8791, V9001; Promega,)Williams' Medium E (Thermo Fisher Scientific, cat. no. 12551032)Luciferin‐CEE, Luciferin‐2B6,

(13) A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term hostVirology journalApril 5, 2016Sa-Ngiamsuntorn K, Wongkajornsilp A, Phanthong P, Borwornpinyo S, Kitiyanant N, Chantratita W, Hongeng S.assessed based on luciferase activity using the P450-glo 1A1, 2B6, 2C9 and 3A4 assays (V8751, V8321, V8791, V9001; Promega, WI). After 3-d incubation period, cells were incubated with Williams’ Media E supplemented

(14) Effect of an ethanol extract of Descurainia sophia seeds on Phase I and II drug metabolizing enzymes and P-glycoprotein activity in vitroBMC Complementary and Alternative MedicineDecember 18, 2015Jin-Mu Yi, Young Ah Kim, You Jin Lee, Ok-Sun Bang, No Soo KimI,CYP1A2,Promega,V8772,Invitrogen,P2792,α-naphthoflavone,Sigma-Aldrich,N5757 ,CYP2C8,Promega,V8782,Invitrogen,PV6138,Montelukast,Cayman,10008318 ,CYP2C9,Promega,V8792,Invitrogen,P2378,Sulfaphenazole,Sigma-Aldrich,S0758 ,CYP2C19,Promega,V8882,Invitrogen,P2864,Ticlopidine,Sigma-Aldrich,T6654 ,CYP2D6,Promega,V8892,Invitrogen,P2283,Quinidine,Sigma-Aldrich,Q3625 ,CYP3A4,Promega,V8802,Invitrogen,P2858,Ketoconazole,Sigma-Aldrich,K1003 Ph

(15) Enhancement of CYP3A4 Activity in Hep G2 Cells by Lentiviral Transfection of Hepatocyte Nuclear Factor-1 AlphaPLoS ONEApril 14, 2014Tsai-Shin Chiang, Kai-Chiang Yang, Ling-Ling Chiou, Guan-Tarn Huang, Hsuan-Shu Leeincubated with respective luciferin substrates (Luciferin-IPA for CYP3A4, V9002; Luciferin-H for CYP2C9, V8791; and Luciferin-CEE for CYP1A1/1B1, V8751) for 1, 4, and 3 h, respectively, at 37°C with occasional mixing

(16) Orally Active Metabotropic Glutamate Subtype 2 Receptor Positive Allosteric Modulators: Structure–Activity Relationships and Assessment in a Rat Model of Nicotine DependenceJournal of Medicinal ChemistryNovember 26, 2012Shyama Sidique et al.assessed using the P450-Glo™ CYP1A2 Assay kit (Promega, P/N V8422), the CYP2C9 Assay kit (Promega, P/N V8792), or the CYP3A4 Assay kit (Promega, P/N V8912) in the presence of human liver microsomes (XenoTech,

(17) Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducersBMC BiotechnologyDecember 1, 2011Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp, Kanda Kasetsinsombat, Sunisa Duangsa-ard, Lalana Nuntakarn, Suparerk Borwornpinyo, Pravit Akarasereenont, Somchai Limsrichamrern, Suradej Hongengassessed based on luciferase activity using the P450-glo 1A1, 1A2, 2C9 and 3A4 assay (V8751, V8771, V8791, V9001; Promega, WI). After 3-d incubation period, cells were incubated with IMDM supplemented with

(18) In vivo liver regeneration potential of human induced pluripotent stem cells from diverse originsScience translational medicineMay 11, 2011Liu H, Kim Y, Sharkis S, Marchionni L, Jang YY.assessed using the pGlo kit (Promega, CYP1A2-V8771, CYP3A4-V8901, CYP2D6-V8891, CYP2C19-V8881, and CYP2C9-V8791) according to the manufacturer's instruction for nonlytic CYP450 activity estimation. iPSC-derived MH

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P450-Glo CYP2C9 Assay

P450-Glo CYP2C9 Assay from Promega

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P450-Glo CYP2C9 Assay from Promega

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