BacTiter-Glo Microbial Cell Viability Assay from Promega

Product Specs
ItemBacTiter-Glo Microbial Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8232
TargetMicrobial Cell Viability
Sample TypeLive Cells
Detectionluminescence
MethodLuminescent
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeastSynthetic and Systems BiotechnologyJune 1, 2026Ling Qin, Shoujie He, Dan Yuan, Yuyang Pan, Zhibo Yan, Mingtao Huangacetyl-coenzyme A synthetase activity using the BacTiter-Glo™ Microbial Cell Viability Assay (Cat No. G8230, Promega, USA) and the Amplex Red Acetyl-Coenzyme A Synthetase Assay Kit (Cat No. S0391S, Beyotime,

(2) Iterative evolution of Saccharomyces cerevisiae enhances recombinant protease secretionSynth Syst BiotechnolApril 25, 2026Xiufang Liu, Mulin Lian, Jiapeng Zeng, Lin Zheng, Mouming Zhao, Mingtao HuangBeijing, China). ATP content was quantified with the BacTiter-Glo™ Microbial Cell Viability Assay (G8230; Promega, Madison, WI, USA), according to the manufacturer's instructions. NAD(H) and NADP(H) levels

(3) Lavender compounds interfere with AI-2 dependent bioluminescence in Vibrio harveyi without affecting LuxS signaling in Campylobacter jejuniiScienceApril 17, 2026Blaž Jug, Dina Jug, Sonja Smole Možina, Anja KlančnikUSA",CAT#12185010 Qubit RNA HS kit,"Thermo Fisher Scientific, USA",CAT#Q32852 BacTiter-Glo reagent,"Promega, USA",CAT# G8230 ,, Deposited data,Deposited data,Deposited data ,, "RNA sequencing data - NCBI, BioProject: PRJNA747749

(4) Metabolic activation of pyruvate cycle by protocatechualdehyde triggers oxidative killing of ampicillin-resistant Escherichia coliMicrobiology SpectrumApril 7, 2026Liting Cheng, Yue Liu, Yifei Ge, Shuhan Yang, Tao Yuan, Sufang Kuanglabel (Meigu Molecular Instruments, Spectramax iD2) by adding 50 μL of BacTiter GloTM reagent (Promega, G8230), which was pre-balanced at room temperature for 15 min, away from light. Three biological replicates

(5) Photothermal Recycling Biosensing for Continuous, Sensitive Molecular QuantificationbioRxivApril 1, 2026Yongchen Tai, Yunshen Li, Wenting Wang, Yang Lu, Ziyan Qian, Mitchell Conover, Josef Neu, Carl Denard, Qiye Zheng, Jing Panplasmid is encapsulated for hepcidin production. BacTiter-Glo™ Microbial Cell Viability Assay kit (G8230) is obtained from Promega. Hardware Setup The imaging system comprises a TIRF Nikon microscope integrated

(6) Dental plaque biofilm transforms host-derived β2-microglobulin into polymorphic fibrils for integration into the biofilm matrixBiofilmDecember 1, 2025Taiki Mori, Eisuke Domae, Mariko Hanaoka, Takeshi Intogrowth profiles were determined using a BacTiter-Glo Microbial Cell Viability Assay Kit (Promega, Cat# G8230) according to the manufacturer's instructions. Bioluminescence was quantified using an Infinite M Plex

(7) Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic miceFEBS Open BioOctober 13, 2025Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, et al.USA). The concentration of ATP generated from PPi was measured using the BacTiter‐Glo kit (Promega, G8230; Madison, WI, USA) [25]. The levels of carbamide and creatinine in the plasma, indicators of renal function,

(8) Inhibition of efflux pumps by FDA-approved drugs oxiconazole and sertaconazole restores antibiotic susceptibility in multidrug-resistant S. aureusAntimicrobial Agents and ChemotherapySeptember 3, 2025Suvendu Ojha, Puja Chatterjee, Tushar Kant Beuriaoxiconazole on cellular ATP levels was assessed using the BacTiter-Glo kit (Promega, Catalog number: G8230) following the manufacturer’s protocol. Cells were cultured to an OD600 ~ 0.3 and treated with 4 µg/mL

(9) Integration of multi-modal measurements identifies critical mechanisms of tuberculosis drug actionCell SystemsAugust 20, 2025William C. Johnson, Ares Alivisatos, Trever C. Smith, Nhi Van, Vijay Soni, Joshua B. Wallach, Nicholas A. Clark, Timothy A. Fitzgerald, Joshua J. Whiteley, Shumin Tan, et al.by a recombinant luciferase enzymatic reaction. Briefly, we added 50 μL of BacTiter-Glo™ (Promega; G8230) to an equal volume of drug-treated cell culture in each well. Plates were rocked gently by hand for

(10) The diverse phenotypic and mutational landscape induced by fluoroquinolone treatmentmSystemsAugust 19, 2025Sayed Golam Mohiuddin, Pouria Kavousi, Diego Figueroa, Sreyashi Ghosh, Mehmet A. Ormanmutant strains were quantified using the BacTiter-Glo Microbial Cell Viability Assay Kit (Catalog# G8230, Promega Corporation, Madison, WI, USA), following the manufacturer’s instructions. Fresh LB medium

(11) A potent phenylalkylamine disrupts mycobacterial membrane bioenergetics and augments bactericidal activity of bedaquilineiScienceJuly 18, 2025Zheng Yen Phua, Ming Li, Azhar Ali, Cedric Cheng Sheng Sheng Cheong, Kai Jie Goh, Marcus Yi Kang Kang Seto, Amos Shi Ying Ying Ng, Jickky Palmae Sarathy, Boon Cher Goh, Mei Lin Go, et al.Proliferation,Promega,Cat. No. G3582 BacTiter-Glo™ Microbial Cell Viability Assay,Promega,"Cat. No. G8230, 8231, 8232, 8233" BacLight™ Bacterial Membrane Potential Kit,ThermoFisher Scientific,Cat. No. B34950 LIVE/DEAD™ BacLight™ Bacterial Viability Kit

(12) Mitochondrial anchor protein Num11 is key to pathogenicity of Candida albicans by affecting mitochondrial function and cell wall maskingVirulenceJune 18, 2025Guangyuan Yang, Xiaojia Niu, Tian Zhuang, Xiaoxiao Zhu, Qianwen Xu, Hongchen Wang, Jing Shao, Changzhong Wang, Yue Yang, Tianming Wang, et al.measurement The intracellular ATP content of each strain was quantified utilizing the BacTiter-Glo™ (G8230, Promega, Beijing, China) microbial cell viability assay kit [59]. After overnight cultivation in YPD

(13) Methylobacterium extorquens PA1 utilizes multiple strategies to maintain formaldehyde homeostasis during methylotrophic growthPLoS GenetJune 9, 2025Zachary T. Hying, Anya M. Rushmer, Chin Yi Loh, Eric L. Bruger, Jannell V. Bazurtoquantification Viability was quantified using the BacTiter-Glo Microbial Cell Viability Assay kit (Promega, Cat No. G8232) according to the manufacturer’s protocol, after validating its reliability by comparing growth measured

(14) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in Borrelia burgdorferi-infected miceElifeMay 20, 2025Qian Yu, Xiaotian Tang, Thomas Hart, Robert Homer, Alexia A Belperron, Linda K Bockenstedt, Aaron Ring, Akira Nakamura, Erol Fikrig,R&D Systems,Cat#DY1274-05, Commercial assay or kit,BacTiter-Glo Microbial Cell Viability Assay kit,Promega,Cat#G8230, Commercial assay or kit,"Mouse MMP 5-Plex Discovery Assay Array (MDMMP-S, P)",Eve Technologies,N/A, Commercial

(15) Skin mycobiota-mediated antagonism against Staphylococcus aureus through a modified fatty acidCurrent BiologyMay 19, 2025Caitlin H. Kowalski, Uyen Thy Nguyen, Susannah Lawhorn, T. Jarrod Smith, Rebecca M. Corrigan, Won Se Suh, Lindsay Kalan, Matthew F. Barbercommercial assays,Critical commercial assays BacTiter Glo™ Microbial Cell Viability Assay,Promega,Cat#G8230 Qiagen DNeasy Blood and Tissue kit,Qiagen,Cat#69504 Zymo DNA Clean Concentrator kit,Zymo Research,Cat#D4004 Zymopure

(16) Metabolic Stress Induced by Quercetin Enhances Dormancy and Persistence in Staphylococcus aureusAntibiotics (Basel)April 22, 2025Dae-Youn Kim, Tae-Jong Kimintracellular ATP levels was assessed using the BacTiter-Glo™ Microbial Cell Viability Assay (catalog number: G8230; Promega Korea Ltd., Seoul, Republic of Korea), according to the manufacturer’s instructions. In brief,

(17) Recombinant ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) decreases vascular calcification and prevents osteomalacia in a rat model of chronic kidney diseaseJBMR PlusApril 16, 2025Kevin O’Brien, Lisa Laurion, Caitlin Sullivan, Jennifer Howe, Angela Malin Lynch, Zhiliang Cheng, Denis Schrier, Hervé Husson, Yves Sabbaghquantified by mixing the reaction mixture in a 1:1 ratio with BactiterGlo detection reagent (Cat. #G8230, Promega). To account for endogenous ATP present in the plasma, a blank reaction was run for each sample

(18) Bradyrhizobium diazoefficiens cultures display phenotypic heterogeneityISME CommunicationsMarch 28, 2025Sukhvir K Sarao, Armaan K Sandhu, Ryan L Hanson, Tanvi Govil, Volker S Brözeltransferred to a polystyrene 96 well plate and 100 μl of BacTiter- Glo Microbial Viability Assay reagent (Cat G8230, Promega) was added. The plate was put on a shaker for 5 min in the dark. Samples were transferred to

(19) Self-growth suppression in Bradyrhizobium diazoefficiens is caused by a diffusible antagonistISME CommunicationsFebruary 17, 2025Armaan Kaur Sandhu, Brady R Fischer, Senthil Subramanian, Adam D Hoppe, Volker S Brözel14. ATP content was measured using the BacTiter-Glo™ Microbial Cell Viability Assay Kit (Promega, Cat.G8230/2). Briefly, agar (500 μl) from five locations was mixed with 750 μl of P/10 (1/10 the peptone and yeast

(20) Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agentmBioDecember 11, 2024Xiaotian Tang, Yingjun Cui, Ushuu Namarra, Xiuqi Tian, Freddie Rivas-Giorgi, Erol Fikrigdetermined as described in Tang et al. (23) using BacTiter-Glo Microbial Cell Viability Assay Kit (Promega, #G8230), which is based on quantitation of the ATP present by measuring luminescence. RNA-seq analysis To

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BacTiter-Glo Microbial Cell Viability Assay from Promega

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BacTiter-Glo Microbial Cell Viability Assay from Promega

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