CellTiter 96 AQueous One Solution Cell Proliferation Assay G3580 from Promega

Product Specs
ItemCellTiter 96 AQueous One Solution Cell Proliferation Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3580
Detection TargetCell Proliferation
MethodColorimetric
DetectionColorimetric
Sample TypeLive Cells
Quantity1,000 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of coilin in bone marrow as a potential neuroblastoma tumor progression marker transcriptionally regulated by MYCNCancer Biol TherDecember 31, 2026Zhixia Yue, Lan Li, Shuguang Liu, Chao Gao, Sidou He, Tianlin Xue, Wen Zhao, Chunying Cui, Chao Duan, Yan Suproportion and intensity of positive cells. Cell proliferation and apoptosis assay An MTS assay kit (G3580, Promega, USA) was used to evaluate the cell viability. NB cells were seeded in 96-well plates (3000

(2) HnRNP C binding to inverted Alu elements protects the transcriptome from pre-mRNA circularizationSci AdvMay 1, 2026Alberto Marini, Consuelo Pitolli, Sabrina Ciccone, Marco Pieraccioli, Noémie Robil, Chiara Naro, Fernando Palluzzi, Manuela Giansanti, Gianpiero Tamburrini, Luciano Giacò, et al.([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; catalog no. G3582, Promega] and performed in 96-well plates. For D341 cell line, 10,000 cells per well were seeded. For

(3) Acid ceramidase inhibition enhances BCL-2 targeting in venetoclax-resistant acute myeloid leukemiaBlood NeoplasiaMay 1, 2026Johnson Ung, Su-Fern Tan, Jeremy J. P. Shaw, Maansi Taori, Tess M. Deddens, Giovana Venancio, McLane M. Montgomery, James T. Hagen, Raphael T. Aruleba, Upendar R. Golla, et al.[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (Promega, Madison, WI, catalog no. G3582) was incubated for 3 hours. Absorbance was measured at 490 nm with the BioTek Cytation 3 plate reader

(4) The TRKB Agonist 7,8-dihydroxyflavone Alleviates DNA Damage and Apoptosis in a Neuronal Cell Model of Friedreich's AtaxiaMol NeurobiolApril 22, 2026Jorge Galán-Cruz, Andrés Vicente-Acosta, Frida Loría, Javier Díaz-Nido, Saúl Herranz-Martíncommercial kit CellTiter96® AQueous One Solution Cell Proliferation Assay, MTS assay (Promega, Cat. No. G3580), was used to determine cell metabolic activity. This is a colorimetric test to quantify the cellular

(5) Keratinocytes biocompatibility, antibacterial and antioxidant properties of porous coacervate phosphate glass fibres and powders loaded with cerium and clove oil: a comparative studyJournal of Materials Chemistry BApril 22, 2026Daniela Moghaddam, Zarin Moghaddam, Eveliny Tomás Sanwlani, Eveliny Tomas-neri, Oluwadunmininu Unalan, Oluwadunmininu Okude, Charlotte A. Hoxha, Charlotte Andrea Hettiarachchilage, Steven J. Hinder, Mark A. Hettiarachchilage, et al.(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, Promega, G3582) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. These tests are commonly

(6) Quantitative Modeling of Lipid Droplet Contribution to Intracellular Drug Distribution and Efficacy of Tyrosine Kinase InhibitorsACS OmegaApril 21, 2026Xin Liu, Sukyung Wooviability was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, G3582). Briefly, cells were seeded in 96-well plates at a density of 3 × 103 cells/well and allowed to adhere

(7) SGLT2 Inhibitor Dapagliflozin Attenuates Cardiomyocyte Injury and Inflammation Induced by PI3Kα-Selective Inhibitor Alpelisib and Fulvestrant Under HyperglycemiaInt J Mol SciApril 17, 2026Vincenzo Quagliariello, Massimiliano Berretta, Matteo Barbato, Fabrizio Maurea, Maria Laura Canale, Andrea Paccone, Irma Bisceglia, Andrea Tedeschi, Marino Scherillo, Jacopo Santagata, et al.(CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Mannheim, Germany; Cat. No. G3582), which provides an indirect measure of metabolically active viable cells. At the end of the treatment

(8) Molecular targeting of the meningioma cell signaling circuit reveals drug vulnerabilities including synergy between sulforaphane and focal adhesion kinase inhibitionBJC RepApril 15, 2026Michelle C. Schwab, Yasmin Kocas, Sarah Gendreizig, Eric Goulin Lippi Fernandes, Tunc F. Ersoy, Roland Coras, Lars Uwe Scholtz, Holger Sudhoff, Ingo Todt, Matthias Simon, et al.Supplementary Table S2. After 48 h a cell viability assay was performed using an MTS assay (Promega, Germany, #G3580). The medium was removed from the treated cells and 100 µL DMEM medium and 10 µL MTS solution were added.

(9) Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21†Biology of ReproductionApril 13, 2026Yuliya Klymenko, Jessica L Kersey, Hunter D Quigley, Shannon M Hawkinsmedium. Proliferation was assessed using CellTiter 96 AQueous One Solution Cell Proliferation Assay (#G3581, Promega, Madison, WI). Absorbance readings were taken at 490 and 630 nm using a Synergy H1 Hybrid Reader

(10) Bacterial protein-oleate complexes induce ferroptosis-like cell death in colorectal cancer cells by disrupting cell membranes and inhibiting the β-catenin-GPX4 axisCell Death DiscovApril 11, 2026Naeem Ullah, Abdelbasset Yabrag, Amjad Ali, Aftab Nadeem(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay (Promega, G3582) and cell permeability was quantified by using Trypan Blue exclusion assay. Cellular ATP content was

(11) Casein kinase 2-mediated phosphorylation of the splicing factor SF3B3 plays a key role in esophageal squamous cell carcinoma progressionPLoS BiolApril 10, 2026Du-Chuang Wang, Jia-Yuan Li, Xiao-Bing Wang, Guo-Sheng Hu, Rui-Chao Nie, Bin Zheng, Yao-Hui He, Wen Liuassay Cell viability was measured by using a CellTiter 96 AQueous one solution cell proliferation assay kit (G3582, Promega) following the manufacturer’s protocol. Briefly, cells were either transfected with siRNAs

(12) Sympathetic nerve–fibroblast crosstalk drives nerve injury, fibroblast activation, and matrix remodeling in pancreatic cancerJCI InsightApril 8, 2026Ariana L. Sattler, Parham Diba, Kevin Hawthorne, Carl Pelz, Joe Grieco, Tetiana Korzun, Bryan Chong, M.J. Kuykendall, Rosalie C. Sears, Daniel L. Marks, et al.phase contrast). Proliferation assay The CellTiter 96 AQueous One Solution Cell proliferation Assay (MTS) (Promega, G3580) was used to assess cell proliferation. 2 × 103 mPSC1 or 6419c5 cells per well were plated in a 96-well

(13) Epigenetic Compound Library Screen Identifies Ibrutinib as an Inhibitor of Ovarian Clear Cell Carcinoma ViabilityCancer MedApril 8, 2026Yue Ma, Kristie-Ann Dickson, Farhana A. Sarker, Amani Alghalayini, Natisha R. Field, Tao Xie, Tali S. Skipper, Anastasia Karafotias, Sarina Briscas, Christine Yee, et al.viability was assessed using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (cat. #G3581, Promega Corporation). Absorbance at 490 nm was measured using a Tecan Infinite 200 PRO microplate reader

(14) Search for Inhibitors against Inflammatory Responses of Microglia in a Meroterpenoid Library Constructed by Synthetic–Biological Manipulation of Biosynthetic PathwaysACS OmegaApril 7, 2026Kanako Takahashi, Zhiyang Quan, Yukari Shigemoto-Mogami, Bei-bei Zhang, Tomohiko Ohwada, Ikuro Abe, Takayoshi Awakawa, Kaoru Satopurchased from Roche (Basel, Switzerland). CellTiter 96 Aqueous One solution cell proliferation assay (G3581) was purchased from Promega (Madison, Wisconsin, USA). Poly-l-lysine (P9155), lipopolysaccharides (LPS)

(15) Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionCell RepApril 7, 2026Manuel Garcia-Moreno, Azman Embarc-Buh, Robin Truman, Marko Noerenberg, Louisa Iselin, Honglin Chen, Caroline E. Lenz, Jeffrey Y. Lee, Kate Dicker, Snehith Dyavari Shetty, et al.viability was also assessed by adding CellTiter96 Aqueous One Solution Cell Proliferation Assay (Promega, #G3580) and measuring absorbance at 490 nm on a microplate reader. Flow cytometry analysis of HIV fitness We

(16) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (Promega #G3582) was used to quantify the cytotoxicity of CAR T-cells. Briefly, GL261 or SB28.B7H3.flag tumor cells

(17) Reactive sulfur species are inactivated and excreted as trimethylsulfonium ion by thiopurine S-methyltransferaseRedox BiolApril 1, 2026Yasunori Fukumoto, Momoka Uchida, Yoshikazu Yamagishi, Natsu Saito, Ayune Watanabe, Rina Mukasa, Ryosuke Kobayashi, Takuro Horii, Izuho Hatada, Yu-Ki Tanaka, et al.additional 24 h. Cell viability was assessed using the CellTiter 96 Aqueous One Solution (Promega, Cat. No. G3582), in accordance with the manufacturer's instructions, as previously described [10]. All samples were

(18) RASA2 deletion rescues immune synapse dysfunction, enhancing CAR T cell efficacy against DMGsJournal for ImmunoTherapy of CancerMarch 30, 2026Jorge Ibanez-Vega, Robert Teis, Jennifer K Ocasio, Peter Chockley, Aamir Ansari, Alejandro Allo Anido, Sanya Mehta, Michaela Meehl, Brooke Prinzing, Meghan Ward, et al.(Luminex). Cytotoxicity assay A CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega; G3580) was used to assess CAR T cell cytotoxicity as previously described.60 Briefly, tumor cells were incubated

(19) HIF1α mediates resistance to radiation and to KRAS inhibitors in pancreatic adenocarcinomaPLoS OneMarch 26, 2026Kevin J. Tu, Sanjit K. Roy, Tami J. Kingsbury, Hem D. Shuklaproliferation was determined by using CellTiter 96® Aqueous One Solution Cell Proliferation Assay (G3582, Promega). Cells were seeded overnight at a density of 2000 cells/well. The manufacturer’s protocol

(20) Integrated Multi-omic Profiling Identifies BRD8/EP400 as a Pivotal Chromatin Module Mediating Anti-HER2 Response in HR+/HER2+ Breast CancerCancer ResearchMarch 26, 2026Ang Gao, Parth H. Khatri, Gui Ma, Peng Liu, Aranzazu Fernandez-Martinez, Kristine Donahue, Yidan Wang, Eui-Jun Kim, Mingshan Hu, Fabao Liu, et al.weeks. Organoid viability was tested by MTS according to the manufacturer’s instructions (Promega, Cat#G3581). Western blot Western blot was performed as previously described20. Briefly, cells were collected

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1/29/2020Non-Radioactive Aqueous Reagent Mallesh KurakulaHighly recommended for student laboratory research. The reagent shows good reproducibility.

CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) From Promega
The CellTiter 96® AQueous One Solution Cell Proliferation Assay from Promega is a colorimetric ... Read More
CellTiter 96® AQueous One Solution Cell Proliferation Assay From Promega
The Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay uses a colorimetric method ... Read More
Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay
Many cell-based assays require the quantification of cell density in order to measure cytotoxicity ... Read More

CellTiter 96 AQueous One Solution Cell Proliferation Assay

CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega

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Promega

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CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) From Promega

CellTiter 96® AQueous One Solution Cell Proliferation Assay From Promega

Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay

CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega

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