Porcine IL-6 DuoSet ELISA DY686 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPorcine IL-6 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY686
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range125.00 - 8000 pg/mL
ReactivityPorcine
Detection TargetIL-6
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterleukin 6

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-Weigandsupernatant of infected PCLS was measured using porcine DuoSet ELISA kits (R&D Systems, Cat. No. DY680, DY686, DY535, and DY690B) according to the manufacturer’s instructions. Samples were diluted 1:2, 1:10, or

(2) Effects of Dietary Marine Sulfated Polysaccharides Derived From Macroalgae on Intestinal Health of Nursery PigsAnim Sci JFebruary 15, 2026Yi-Chi Cheng, Yesid R. Garavito-Duarte, Maria Garcia Suarez, Maria A. Rodríguez, Sung Woo Kimconcentration of IL‐6 was measured by following instructions of the Porcine IL‐6 DuoSet ELISA Kit (#DY686, R&D Systems), as previously described by Jang and Kim (2019). The absorbance was read at wavelengths

(3) Effect of a trub, hops, and yeast mixture on jejunal mucosal immune status, oxidative stress status, structure, and growth performance of nursery pigsJ Anim SciDecember 10, 2025Alexa R Gormley, Zixiao Deng, Brock Ashburn, Robert W Bryant, Sung Woo Kimexpressed as pg/mg protein. The IL-6 concentration was measured using the Porcine IL-6 DuoSet ELISA kit (#DY686, R&D Systems) following Deng et al. (2023a). The samples were not diluted prior to analysis, following

(4) Specific Assay Protocols for Porcine Single-Eye Retinal Pigment Epithelium Concerning Oxidative Stress and InflammationInternational Journal of Molecular SciencesAugust 29, 2025Philipp Dörschmann, Marie Prinz, Greta Schmitkall, Johann Roider, Alexa KlettnerTNF secretion. They were applied in Porcine IL-6 DuoSet ELISA (R&D systems, Minneapolis, MN, USA; #DY686), Porcine IL-8/CXCL8 DuoSet ELISA (R&D systems, Minneapolis, MN, USA; #DY535), and Porcine TNF-alpha DuoSet ELISA

(5) Age-dependent differences in type I interferon, IL-12 and pro-inflammatory cytokine production by porcine peripheral blood mononuclear cells in response to pseudorabies virus-infected cellsFront ImmunolJuly 3, 2025Manon S. C. Claeys, Simon G. A. L. Brabant, Stijn Rosschaert, Cliff Van Waesberghe, Herman W. FavoreelIL-1β, IL-12, IL-6, TNF-α, IL-10 ELISA’s were ordered from R&D systems (respectively DY681, DY912, DY686, DY690B, DY693B) and carried out following the manufacturer’s instructions. The optical density was

(6) Impacts of replacing soybean meal with processed soybean meal on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliAnimal BioscienceApril 1, 2025Zixiao Deng, Hyunjun Choi, Sung Woo Kimpg/mL of protein. The concentration of IL-6 was measured by using the Porcine IL-6 DuoSet ELISA Kit (#DY686; R&D Systems), with samples diluted 1:5 in reagent diluent. Absorbance readings were taken at 450 nm

(7) Impacts of non-starch polysaccharide sources with enzymes influencing intestinal mucosa-associated microbiota and mucosal immunity of nursery pigs on growth and carcass traits at market weightJournal of Animal Science and BiotechnologyApril 1, 2025Jonathan T. Baker, Zixiao Deng, Alexa R. Gormley, Sung Woo KimIL-6 in jejunal mucosa was measured by following instructions of the Porcine IL-6 DuoSet ELISA Kit (DY686, R&D Systems, Minneapolis, MN, USA) as described by Duarte et al. [34]. The concentration of IL-6 was

(8) Influence of a Very High-Molecular Weight Fucoidan from Laminaria hyperborea on Age-Related Macular Degeneration-Relevant Pathomechanisms in Ocular Cell ModelsMarine DrugsFebruary 25, 2025Philipp Dörschmann, Georg Kopplin, Tabea Thalenhorst, Charlotte Seeba, Sadia Fida Ullah, Vaibhav Srivastava, Johann Roider, Alexa KlettnerIL-6 and IL-8 were used for RPE samples, all bought from R&D Systems (Minneapolis, MN, USA; # DY293B; #DY686; #DY535). All kits were used according to the manufacturer’s instructions. Standard calibration curves

(9) Altered hepatic metabolism mediates sepsis preventive effects of reduced glucose supply in infected preterm newbornseLifeFebruary 24, 2025Bæk O, Muk T, Wu Z, Ye Y, Khakimov B, Casano AM, Gangadharan B, Bilic I, Brunse A, Sangild PT, et al.cytokines using enzyme-linked immunosorbent assay (ELISA), TNF-α (DY690B), IL-10 (DY693B) and IL-6 (DY686, all porcine DuoSet, R&D systems, Abingdon, UK). Plasma samples at planned euthanasia or human endpoints

(10) Investigation of the nutritional and functional roles of a microencapsulated blend of botanicals on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceFebruary 15, 2025Yesid Garavito-Duarte, Andrea Bonetti, Benedetta Tugnoli, Hyunjun Choi, Andrea Piva, Ester Grilli, Sung Woo Kimpg/mg protein. The interleukin-6 (IL-6) content was determined with the Porcine IL-6 Duoset ELISA kit (#DY686, intra-assay CV of 7% and inter-assay CV of 10%, R&D systems), and interleukin-8 (IL-8) content with

(11) Efficacy of ground herb-based and essential oil-based phytobiotics on the intestinal health and performance of nursery pigs challenged with F18+ Escherichia coliJournal of Animal ScienceJanuary 31, 2025Yesid Garavito-Duarte, Marcos Elias Duarte, Sung Woo Kimprotein. The concentration of IL-6 was determined by employing the Porcine IL-6 DuoSet ELISA kit (#DY686, R&D Systems) in accordance with the method outlined by Deng et al. (2023). Similarly, for the measurement

(12) Saccharomyces yeast postbiotics mitigate mucosal damages from F18+ Escherichia coli challenges by positively balancing the mucosal microbiota in the jejunum of young pigsAnimal MicrobiomeDecember 20, 2024Alexa R. Gormley, Marcos Elias Duarte, Zixiao Deng, Sung Woo Kimfinal concentration of TNF-α was expressed as pg/mg protein. Using the Porcine IL-6 DuoSet ELISA kit (#DY686, R&D Systems) following Deng et al. [76]. Using the Porcine IL-8/CXCL8 DuoSet ELISA kit (#DY535, R&D Systems

(13) Efficacy of Saccharomyces yeast postbiotics on cell turnover, immune responses, and oxidative stress in the jejunal mucosa of young pigsScientific reportsAugust 20, 2024Duarte ME, Kim SW.#23227, Thermo Fisher Scientific Inc., Rockford, IL, USA), interleukin 6 (Porcine IL-6 DuoSet ELISA, #DY686, R&D System Inc. Minneapolis, MN, USA), IL-8 (Porcine IL-8/CXCL8 DuoSet ELISA, #DY535, R&D System Inc.),

(14) The lipopolysaccharide structure affects the detoxifying ability of intestinal alkaline phosphatasesBMC veterinary researchAugust 10, 2024Vermeire B, Walsh M, Cox E, Devriendt B.

(15) Nutritional and functional roles of β-mannanase on intestinal health and growth of newly weaned pigs fed two different types of feedsJournal of Animal ScienceJuly 24, 2024Jonathan T Baker, Zixiao Deng, Adebayo Sokale, Brent Frederick, Sung Woo KimIL-6 in jejunal mucosa was measured by following instructions of the Porcine IL-6 DuoSet ELISA Kit (DY686, R&D Systems, Minneapolis, MN) as described by Duarte et al. (2021). The concentration of IL-6 was described

(16) Immediate inflammatory response to mechanical circulatory support in a porcine model of severe cardiogenic shockIntensive Care Medicine ExperimentalApril 22, 2024Emilie Gregers, Peter H. Frederiksen, Nanna L. J. Udesen, Louise Linde, Ann Banke, Amalie L. Povlsen, Jeppe P. Larsen, Christian Hassager, Lisette O. Jensen, Jens F. Lassen, et al.amyloid A (SAA). IL-6 and -8 concentrations were determined by ELISAs from the R&D systems (Duosets DY686 and DY535, respectively) which use goat anti-pig IL6/-8 for detection. Samples were run in duplicates

(17) Transcriptional Host Responses to Infection with Streptococcus suis in a Porcine Precision-Cut Lung Slice Model: Between-Strain Differences Suggest Association with Virulence PotentialPathogensDecember 19, 2023Yenehiwot Berhanu Weldearegay, Louise Brogaard, Andreas Nerlich, Désirée Schaaf, Peter M. H. Heegaard, Peter Valentin-Weigandwell as mock-infected PCLS. Analysis was performed using the sandwich ELISA kits DY681 (IL-1β) and DY686 (IL-6) (R&D Systems, Abingdon, UK) following the manufacturer’s instructions. Briefly, goat anti-pig

(18) Fluid restrictive resuscitation with high molecular weight hyaluronan infusion in early peritonitis sepsisIntensive Care Medicine ExperimentalSeptember 21, 2023Annelie Barrueta Tenhunen, Jaap van der Heijden, Paul Skorup, Marco Maccarana, Anders Larsson, Anders Larsson, Gaetano Perchiazzi, Jyrki TenhunenELISAs were used for the determination of TNF-α and interleukin-6 (IL-6) in plasma (DY690B (TNF-α), DY686 (IL-6), R&D Systems, Minneapolis, MN, USA). The ELISAs had total coefficient of variations (CV) of approximately

(19) Predicting cytokine kinetics during sepsis; a modelling framework from a porcine sepsis model with live Escherichia coliCytokineSeptember 1, 2023Bahnasawy SM, Skorup P, Hanslin K, Lipcsey M, Friberg LE, Nielsen EI.

(20) Using Recombinant Superoxide Dismutase to Control Oxidative Stress in the Gastrointestinal Tract of Cyclic Heat-Stressed PigsAnimals (Basel)August 21, 2023Hieu Huu Le, Weicheng Zhao, John Barton Furness, Majid Shakeri, Kristy DiGiacomo, Eugeni Roura, David Renaudeau, Nicolas Kurt Gabler, Brian Joseph Leury, Frank Rowland Dunshea, et al.manufacturer’s instructions (R&D Systems, Minneapolis, MI, USA): interleukin 1β (Cat DY681), interleukin 6 (Cat DY686), interleukin 10 (Cat DY693B), and tumour necrosis factor-α (Cat DY690B) (In Vitro Technologies, Noble

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IL-6 ELISA

Porcine IL-6 DuoSet ELISA

Porcine IL-6 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Porcine IL-6 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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