MAGIC RED™ CATHEPSIN B KIT ICT937 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemMAGIC RED™ CATHEPSIN B KIT
CompanyBio-Rad
Price $301.00Supplier Page View Company Product Page
Catalog NumberICT937
Detection TargetCATHEPSIN B
Quantity25 TESTS
ApplicationsImmunofluorescence
Conjugate/Tag/LabelMAGIC RED™ (Red Fluorescence)
TypeKits

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Loss of the lysosomal protein CLN3 triggers c-Abl-dependent YAP1 pro-apoptotic signalingEMBO ReportsNovember 6, 2025Neuza Domingues, Alessia Calcagni’, Sofia Freire, Joana Pires, Ricardo Casqueiro, Ivan L Salazar, Niculin Joachim Herz, Tuong Huynh, Katarzyna Wieciorek, Tiago Fleming Outeiro, et al.Nuclear/cytosol fractionation kit,Abcam,AB289882 FITC,Merck,60842-46-8 iScript cDNA synthesis kit,Bio-Rad,170-8891 Alexa Fluor 647,Thermo Fisher,D22914 Magic-red,Bio-Rad,ICT937 GasPaKTM EZ – Anaerobe Gas Generating pouch system,BD,260683 Zeiss LSM 710,Carl Zeiss AG, BioRender,BioRender,BioRender Reagents

(2) SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesisNat CommunOctober 3, 2025Kaushal Asrani, Adrianna Amaral, Juhyung Woo, Sanaz Nourmohammadi Abadchi, Thiago Vidotto, Eddie Imada, Alyza Skaist, Kewen Feng, Hans B. Liu, Mithila Kasbe, et al.Bortezomib (#2204S, Cell Signaling), Cycloheximide (#2112, Cell Signaling), Magic Red Cathepsin B kit (#ICT937, BIO-RAD), CYTO-ID (#ENZ-51031, Enzo), Pierce anti-HA-magnetic beads (#88837, Thermo Fisher Scientific),

(3) PERK and IRE1α promote exosome secretion via blocking lysosomal degradation of multiple vesicular bodyJournal of Biological ChemistryJuly 21, 2025Shixin Zhou, Zihan Zhou, Si Chen, Ming Wang, Likun WangCat#406412), and anti-Rat Alexa Fluor 488 (BioLegend, Cat#405418). Magic Red assay Magic Red (Bio-Rad, Cat#ICT937) was used to analyze the CTSB enzymatic activity in lysosomes as an indicator of lysosome function.

(4) Pathogenic variants in BORCS5 Cause a Spectrum of Neurodevelopmental and Neurodegenerative Disorders with Lysosomal DysfunctionmedRxivMay 7, 2025Niccolò E. Mencacci, Georgia Minakaki, Reza Maroofian, Raffaella De De Pace, Adeline Paimboeuf, Patrick Shannon, David Chitayat, Francesca Magrinelli, Wesley J. Peng, Diptaman Chatterjee, et al.incubated with LysoTracker Red DND-99 (L7528; Thermo Fisher Scientific) (50 nM), Magic Red cathepsin B (ICT937; BioRad) (1:2,000), or Calcein AM-488 (20 nM) for 30 min in fresh culture media. Cells were imaged after

(5) Drosophila aux orchestrates the phosphorylation-dependent assembly of the lysosomal V-ATPase in glia and contributes to SNCA/α-synuclein degradationAutophagyJanuary 29, 2025Shiping Zhang, Linfang Wang, Shuanglong Yi, Yu-Ting Tsai, Yi-Hsuan Cheng, Yu-Tung Lin, Chia-Ching Lin, Yi-Hua Lee, Honglei Wang, Shuhua Li, et al.brains were incubated in Magic Red Cathepsin B using the Magic Red® Cathepsin B assay kits (Bio-Rad, ICT937) solution for 5 min at 25°C. The Magic Red Cathepsin B was reconstituted with DMSO to form the stock

(6) Lysosomal activity depends on TRPML1-mediated Ca2+ release coupled to incoming vesicle fusionsJournal of Biological ChemistryDecember 1, 2024Arindam Bhattacharjee, Hussein Abuammar, Gábor JuhászCat# D22914), vinblastine (Sigma Cat# V1377), Magic Red cathepsin B activity assay kit (Bio-Rad Cat# ICT937), Mirus Trans-IT LT1 transfection reagent (Mirus Cat# MIR 2300), OptiMEM (Thermo Cat# 31985070), polyethylenimine

(7) Renal antiporter ClC-5 regulates collagen I/IV through the β-catenin pathway and lysosomal degradationLife science allianceJuly 1, 2024Durán M, Ariceta G, Semidey ME, Castells-Esteve C, Casal-Pardo A, Lu B, Meseguer A, Cantero-Recasens G.reinforce these results, we also tested lysosomal enzymatic activity using Magic Red Cathepsin B Kit (#ICT938; Bio-Rad). Our data revealed a 30% decrease in Magic Red fluorescence in CLCN5 KD compared with control

(8) Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy modelsTranslational neurodegenerationJune 13, 2023Drobny A, Boros FA, Balta D, Prieto Huarcaya S, Caylioglu D, Qazi N, Vandrey J, Schneider Y, Dobert JP, Pitcairn C, et al.then washed out, and cells were pulse chased with cell-permeable substrates MagicRed (RR2) (BioRad; #ICT938) for CTSB activity and MagicRed (FR2) (BioRad; #ICT942) for CTSL activity as indicated in the manual

(9) Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer's diseaseCellular and molecular life sciences : CMLSMay 5, 2023Hua J, Garcia de Paco E, Linck N, Maurice T, Desrumaux C, Manoury B, Rassendren F, Ulmann L.24-well plate containing cover slips and incubated with the Magic Red CatB substrate (1:250, Bio-Rad, ICT938) for 2 h. Cells were then fixed and mean fluorescence intensity in cells was quantified using the ImageJ

(10) Genetic models of cleavage-reduced and soluble TREM2 reveal distinct effects on myelination and microglia function in the cuprizone modelJournal of neuroinflammationFebruary 8, 2023Beckmann N, Neuhaus A, Zurbruegg S, Volkmer P, Patino C, Joller S, Feuerbach D, Doelemeyer A, Schweizer T, Rudin S, et al.37 °C in 5% CO2. On the next day, Magic Red substrate from the Magic red Cathepsin B Kit (Bio-Rad, ICT938) was diluted in distilled water (dH2O) (Invitrogen, 10977055) containing Hoechst (1:4000) according

(11) OCA7 is a melanosome membrane protein that defines pigmentation by regulating early stages of melanosome biogenesisThe Journal of biological chemistryDecember 1, 2022Beyers WC, Detry AM, Di Pietro SM.cells, media were aspirated from the dish, and cells were incubated with 2 ml acridine orange (Bio-Rad, ICT937) diluted to 1 μM in KGM Gold media for 30 min in a humidified cell culture incubator at 37 °C and 5%

(12) Lack of bombesin receptor-activated protein attenuates bleomycin-induced pulmonary fibrosis in miceLife science allianceNovember 1, 2022Wang H, Zhang W, Liu R, Zheng J, Yao X, Chen H, Wang J, Weber HC, Qin X, Xiang Y, et al.components. We measured the activity of cathepsin B activity using Magic Red Cathepsin assay kit (Cat. no.: ICT937; Bio-Rad) according to the manufacturer’s protocol. Briefly, primary lung fibroblasts were isolated

(13) DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletionThe EMBO JournalMay 16, 2022Maeve Long et al.the manufacturer’s guidelines. Cathepsin‐reactive lysosomes were visualised using Magic Red (Bio‐Rad, ICT937) according to the manufacturer’s guidelines and as described (Bright et al, 2016) with minor modifications.

(14) Impact of α-Synuclein Fibrillar Strains and β-Amyloid Assemblies on Mouse Cortical Neurons Endo-Lysosomal LogisticseneuroMay 1, 2022Qiao-Ling Chou, Ania Alik, François Marquier, Ronald Melki, François Treussart, Michel Simonneauobservation, with LysoTracker Deep Red (ref. L12492, ThermoFisher) or Magic Red Cathepsin B substrate (ref. ICT937, Bio-Rad). These dye molecules have an emission spectrum within the similar range than the one of FND.

(15) Enhanced enzymatic production of cholesteryl 6ʹ-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pyloriJournal of Biomedical ScienceDecember 1, 2021Sasikala Muthusamy, Hau-Ming Jan, Ming-Yen Hsieh, Soumik Mondal, Wen-Chun Liu, Yi-An Ko, Wei-Yuan Yang, Kwok-Kong Tony Mong, Guang-Chao Chen, Chun-Hung LinB staining kit (Invitrogen; V34404), Magic Red™ cathepsin B staining kit (Bio-Rad, California, USA; ICT937), Oregon Green™ 488 BAPTA-5N (Invitrogen California, USA; O6812), Dextran Alexa Fluor™ 568 (Invitrogen

(16) Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruptionCurrent biology : CBApril 12, 2021Adams SD, Csere J, D'angelo G, Carter EP, Romao M, Arnandis T, Dodel M, Kocher HM, Grose R, Raposo G, et al.23227) as per the manufacturer’s instructions. Magic Red assay The Magic Red Cathepsin B kit (Bio-Rad, ICT937) was used to analyze the protease activity of Cathepsin B in lysosomes as a proxy to lysosome function.

(17) LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived NeuronsFrontiers in NeuroscienceMay 15, 2020Anwesha Sanyal, Hailey S. Novis, Emile Gasser, Steven Lin, Matthew J. LaVoiewell in 96-well blackwall clear-bottom plates (Greiner), labeled with 1 µM MagicRedTM dye (Bio-Rad, ICT937 and ICT 941) for 10 min and 20 ng/ml of Hoechst prior to rinsing 2x with HBSS buffer. Total fluorescence

(18) Spatially and temporally defined lysosomal leakage facilitates mitotic chromosome segregationNature communicationsJanuary 13, 2020Hämälistö S, Stahl JL, Favaro E, Yang Q, Liu B, Christoffersen L, Loos B, Guasch Boldú C, Joyce JA, Reinheckel T, et al.Alexa Fluor® 488 for 16 h, washed and chased for 2 h. Magic Red Cathepsin B substrate (AbD Serotec, ICT937) was added at 1:25 (vol:vol) with indicated treatments and cells were incubated for 15 min at 37 °C

(19) mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulationThe Journal of clinical investigationDecember 2, 2019Asrani K, Murali S, Lam B, Na CH, Phatak P, Sood A, Kaur H, Khan Z, Noë M, Anchoori RK, et al.Recombinase, Akt1 (Myr) and Akt2 (Myr) adenoviruses (Vector Biolabs), Magic Red Cathepsin B Kit (catalog ICT937, Biorad). 4XCLEAR-luciferase reporter was a gift from Albert La Spada (Addgene plasmid 66880) (37). Histology

(20) Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysisAutophagySeptember 1, 2019Fernandez-Mosquera L, Yambire KF, Couto R, Pereyra L, Pabis K, Ponsford AH, Diogo CV, Stagi M, Milosevic I, Raimundo N.(Marienfeld, 0101060) the day before the experiment. The incubation of the cells with Magic Red (Bio-Rad, ICT937) took 20 min, after which the coverslip was washed in warm imaging buffer and then placed on a coverslip

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MAGIC RED™ CATHEPSIN B KIT

MAGIC RED™ CATHEPSIN B KIT

MAGIC RED™ CATHEPSIN B KIT

MAGIC RED™ CATHEPSIN B KIT

MAGIC RED™ CATHEPSIN B KIT

MAGIC RED™ CATHEPSIN B KIT from Bio-Rad (Formerly AbD Serotec)

Bio-Rad

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MAGIC RED™ CATHEPSIN B KIT from Bio-Rad (Formerly AbD Serotec)

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