Mouse CXCL12/SDF-1 DuoSet ELISA DY460 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse CXCL12/SDF-1 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY460
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range46.90 - 3000 pg/mL
ReactivityMouse
Detection TargetCXCL12/SDF-1
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namechemokine (C-X-C motif) ligand 12
NCBI Gene AliasesPbsf, Scyb12, Sdf1, Tlsf, Tpar1

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Resilient Calvarial Bone Marrow Supports Retinal Repair in Type 2 DiabetesAdvanced ScienceJanuary 4, 2026Bright Asare‐Bediako, Sergio Li Calzi, Julia G. Behnsen, Ram Prasad, Magdalena Blaszkiewicz, Yvonne Adu‐Rutledge, Robert F. Rosencrans, Jason L. Floyd, Ashley Rennhack, Denise Stanford, et al.ELISA Samples were analysed for CXCL12 levels using the Mouse CXCL12/SDF‐1 DuoSet ELISA kit (R&D Systems, Cat# DY460) according to manufacturer's instructions. 5.10 Colony‐Forming Unit (CFU) Assay CFU assay was performed

(2) Chronic social defeat stress induces meningeal neutrophilia via type I interferon signaling in male miceNature CommunicationsSeptember 1, 2025Stacey L. Kigar, Mary-Ellen Lynall, Allison E. DePuyt, Robert Atkinson, Virginia H. Sun, Joshua D. Samuels, Nicole E. Eassa, Chelsie N. Poffenberger, Michael L. Lehmann, Samuel J. Listwak, et al.kits for murine CXCL1 (Cat #900-K127, PreproTech), CXCL2 (Cat #900-K152, PreproTech), and CXCL12 (Cat #DY460, R&D Systems) according to manufacturer’s instructions. To compensate for sample plasma effects, assay

(3) Ex Vivo Model of Breast Cancer Cell Invasion in Live Lymph Node TissueACS Pharmacology & Translational ScienceMarch 14, 2025Katerina Morgaenko, Abhinav Arneja, Alexander G. Ball, Audrey M. Putelo, Jennifer M. Munson, Melanie R. Rutkowski, Rebecca R. PompanoMinneapolis, MN, USA). ELISAs were for CCL21 (catalog no. DY457), CCL19 (DY440), CCL1 (DY845), CXCL12 (DY460), and CXCL13 (DY470) according to the manufacturer’s protocol. For the measurement of intranodal IL-21

(4) KLF5 enhances CXCL12 transcription in adipose-derived stem cells to promote endothelial progenitor cells neovascularization and accelerate diabetic wound healingCell Mol Biol LettMarch 4, 2025Yunjia Xie, Xuejun Ni, Xiaofen Wan, Nating Xu, Lu Chen, Chensheng Lin, Xi Zheng, Beichen Cai, Qian Lin, Ruonan Ke, et al.concentration of secreted CXCL12 in the ADSCs culture medium were measured with the DuoSet ELISA kit (cat. DY460, R&D Systems, USA) following the manufacturer’s instructions. Briefly, 96-well plates were coated with

(5) Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokineMolecular metabolismMarch 1, 2025Agueda-Oyarzabal M, Isidor MS, Plucińska K, Ingerslev LR, Dmytriyeva O, Petersen PSS, Laftih S, Pontoppidan AB, Henningsen JB, Rupar K, et al.100 μl of plasma was added in duplicates onto the ELISA plate. CXCL12 ELISA assays (R&D systems, Cat. DY460) were performed according to the manufacturer's instructions. 2.8 RNAscope in situ hybridization and immunohistochemistry

(6) Healthy longevity-associated protein improves cardiac function in murine models of cardiomyopathy with preserved ejection fractionCardiovascular diabetologyNovember 5, 2024Alvino VV, Slater S, Qiu Y, Cattaneo M, Mohammed KAK, Gate S, Sekar V, Puca AA, Madeddu P.using the DuoSet ELISA assay kit according to the manufacturer's instructions (R&D Systems, cat n# DY460, UK). Statistical analyses The data are presented as individual values and means ± standard errors

(7) Ultralow-dose irradiation enables engraftment and intravital tracking of disease initiating niches in clonal hematopoiesisScientific ReportsSeptember 3, 2024Kevin Lee, Wimeth Dissanayake, Melissa MacLiesh, Cih-Li Hong, Zi Yin, Yuko Kawano, Christina M. Kaszuba, Hiroki Kawano, Emily R. Quarato, Brian Marples, et al.assay CXCL12(SDF-1) levels in the bone marrow interstitial fluid were assessed using an ELISA DuoSet kit (R&D system DY460) and DuoSet ELISA Ancillary Reagent kit (R&D system DY008B). Mice (wild-type littermates) between the

(8) Ex vivo model of breast cancer cell invasion in live lymph node tissuebioRxiv : the preprint server for biologyJuly 22, 2024Morgaenko K, Arneja A, Ball AG, Putelo AM, Munson JM, Rutkowski MR, Pompano RR.Minneapolis, MN, USA). ELISAs were for CCL21 (catalog no. DY457), CCL19 (DY440), CCL1 (DY845), CXCL12 (DY460) and CXCL13 (DY470) according to the manufacturer’s protocol. For measurement of intranodal IL-21 levels,

(9) CNS-wide repopulation by hematopoietic-derived microglia-like cells corrects progranulin deficiency in miceNature communicationsJuly 5, 2024Colella P, Sayana R, Suarez-Nieto MV, Sarno J, Nyame K, Xiong J, Pimentel Vera LN, Arozqueta Basurto J, Corbo M, Limaye A, et al.

(10) PU.1 alleviates the inhibitory effects of cigarette smoke on endothelial progenitor cell function and lung-homing through Wnt/β-catenin and CXCL12/CXCR4 pathwaysTobacco induced diseasesJanuary 1, 2024He X, Cui Y, Li T, Luo L, Zeng Z, Ma Y, Chen Y.Enzyme-linked immunosorbent assay (ELISA) CXCL12 levels in the supernatant of BM flush fluid were measured using an ELISA kit (DY460, R&D Systems, USA) as described by the manufacturer’s instructions. Statistical analysis Continuous

(11) G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulationStem Cell Research & TherapyDecember 1, 2022Jaime M. Brozowski et al.monolayers were collected and analyzed for CXCL12 protein using the CXCL12 DuoSet ELISA kit (R&D Systems, DY460), as per instructions. Microcomputed tomography (µCT) For 8–12-week-old mice, µCT imaging was used

(12) Biased action of the CXCR4-targeting drug plerixafor is essential for its superior hematopoietic stem cell mobilizationCommunications BiologyDecember 1, 2021Astrid S. JÃ¸rgensen, Viktorija Daugvilaite, Katia De Filippo, Christian Berg, Masa Mavri, Tau Benned-Jensen, Goda Juzenaite, Gertrud HjortÃ¸, Sara Rankin, Jon VÃ¥benÃ¸, et al.software. ELISA Supernatant from bone marrow, spleen, and serum from blood was used to test CXCL12 by ELISA (R&D Systems, DY460). Molecular modeling The Schrödinger Small-Molecule Drug Discovery Suite (release 2019-3)75 was used

(13) HMGB1 promotes CXCL12-dependent egress of murine B cells from Peyer's patches in homeostasisEuropean journal of immunologyAugust 1, 2021Spagnuolo L, Puddinu V, Boss N, Spinetti T, Oberson A, Widmer J, Mottas I, Hotz C, Bianchi ME, Uguccioni M, et al.

(14) Lung Marginated and Splenic Murine Resident Neutrophils Constitute Pioneers in Tissue-Defense During Systemic E. coli ChallengeFrontiers in ImmunologyApril 19, 2021Goda Juzenaite, Judith Secklehner, Juho Vuononvirta, Yoseph Helbawi, John B. G. Mackey, Charlotte Dean, James A. Harker, Leo M. Carlin, Sara Rankin, Katia De Filippo(R&D Systems) were used: Mouse CXCL1 (DY493), Mouse CXCL2 (DY492), Mouse IL-1β (DY401), and Mouse CXCL12 (DY460). Statistical Analysis Statistical analysis was performed using GraphPad Prism 8 (GraphPad Software,

(15) A Selective Neutraligand for CXCL12/SDF-1α With Beneficial Regulatory Functions in MRL/Lpr Lupus Prone MiceFrontiers in pharmacologyJanuary 1, 2021Schall N, Daubeuf F, Marsol C, Gizzi P, Frossard N, Bonnet D, Galzi JL, Muller S.CXCL12 After Intravenous Administration Into Mice The ELISA kit for mouse CXCL12 (R&D Systems, ref. DY460; kit range: 46–3,000 pg/mL; sensitivity: 23 pg/mL) was used according to the manufacturer’s instructions. In

(16) An injectable hydrogel-formulated inhibitor of prolyl-4-hydroxylase promotes T regulatory cell recruitment and enhances alveolar bone regeneration during resolution of experimental periodontitisFASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 1, 2020Nagai K, Ideguchi H, Kajikawa T, Li X, Chavakis T, Cheng J, Messersmith PB, Heber-Katz E, Hajishengallis G.ligand 12 (CXCL12) were determined using ELISA kits (cat# DY293B and DY350 for human assays and cat# DY460 and DY493 for mouse assays; R&D Systems, Minneapolis, MN, USA). Resolution of ligature-induced periodontitis

(17) The longevity-associated variant of BPIFB4 improves a CXCR4-mediated striatum-microglia crosstalk preventing disease progression in a mouse model of Huntington's diseaseCell death & diseaseJuly 18, 2020Di Pardo A, Ciaglia E, Cattaneo M, Maciag A, Montella F, Lopardo V, Ferrario A, Villa F, Madonna M, Amico E, et al.

(18) Cathelicidin-related Antimicrobial Peptide Contributes to Host Immune Responses Against Pulmonary Infection with Acinetobacter baumannii in MiceImmune NetworkMay 20, 2020Min-Jung Kang, Ah-Ra Jang, Ji-Yeon Park, Jae-Hun Ahn, Tae-Sung Lee, Dong-Yeon Kim, Do-Hyeon Jung, Eun-Jung Song, Jung Joo Hong, Jong-Hwan ParkNo. DY417), IL-1β (Cat. No. DY401), CXCL1 (Cat. No. DY453), CXCL2 (Cat. No. DY452), CXCL12 (Cat. No. DY460), and CCL2 (Cat. No. DY479) from the BAL fluid of A. baumannii-infected mice or culture supernatant

(19) Regulatory T cells confer a circadian signature on inflammatory arthritisNature communicationsApril 3, 2020Hand LE, Gray KJ, Dickson SH, Simpkins DA, Ray DW, Konkel JE, Hepworth MR, Gibbs JE.

(20) Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammationMolecular metabolismApril 1, 2020Xu M, Ge C, Qin Y, Lou D, Li Q, Feng J, Wu Y, Hu L, Wang B, Tan J.(Cat#: DY440), MDC (Cat#: MCC220), CX3CL1 (Cat#: MCX310), CXCL10 (Cat#: DY466-05), and CXCL12 (Cat#: DY460) ELISA kits were purchased from R&D Systems (Shanghai, China) and used according to the product specifications.

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CXCL12/SDF-1 ELISA

Mouse CXCL12/SDF-1 DuoSet ELISA

Mouse CXCL12/SDF-1 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Mouse CXCL12/SDF-1 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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