Stop Solution 2N Sulfuric Acid from R&D Systems, a Bio-Techne Brand

Product Specs
ItemStop Solution 2N Sulfuric Acid
CompanyR&D Systems, a Bio-Techne Brand
Price $175.00Supplier Page View Company Product Page
Catalog NumberDY994
Quantity1 Pack
ApplicationsELISA
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Development of a DUX4-targeting antibody oligonucleotide conjugate as a therapy for FSHDNucleic Acids ResApril 17, 2026Barbora Malecova, David Sala, Garineh M Melikian, Rachel Johns, Gulin Erdogan, Marc Hartmann, Maryam Jordan, Joel Danny Arias, Arvind Bhattacharya, Qingying Meng, et al.temperature, followed by the addition of 25 µL/well of 2N sulfuric acid stop solution (R&D Systems DY994). Plates were read at 450 nm using a 570 nm reference wavelength. Wells containing capture and secondary

(2) Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammationbioRxivMarch 14, 2026Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A. Han, Jonathan G. Van Vranken, Allison S. Walker, Timothy Rhoads, Snehal N. Chaudharideveloped using TMB substrate (R&D Systems, #DY999B) and stopped with stop solution (R&D Systems, #DY994). Absorbance was measured at 450 nm with wavelength correction at 540 nm. Concentrations were calculated

(3) Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discoveryScientific ReportsFebruary 27, 2026Emilia Sinderewicz, Maria Dąbkowska, Anna Sarnowska, Joanna Staszkiewicz-Chodor, Dorota Mystkowska, Piotr Holak, Igor Drozd, Magdalena Chodkowska-Michalowska, Monika Rytel, Edyta Paczkowska, et al.determined using a commercially available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept constant

(4) Kinetics of local C3 production orchestrates neutrophil recruitment in lung injurybioRxivFebruary 21, 2026Rafael Aponte Aponte Alburquerque, Josue I. Hernandez, Aasritha Nallapu, Marick Starick, Neelou Etesami, Sandip K. Mukherjee, Ayse Naz Ozanturk, Jose Vazquez, Allison Chang, Ashley Zheng, et al.incubated at room temperature (RT) for 10 min. To stop the reaction, 1 M sulfuric acid (50 μl per well; #DY994, R&D Systems) was added, and optical density was measured at 450 nm using an Epoch Microplate Spectrophotometer

(5) A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [89Zr]Zr-IL-23p19 antibodyScientific ReportsNovember 29, 2025Nare Ko, Jung Won Ahn, Dong Yun Lee, Jin Hwa Chung, Seung Jun Oh, Hyori Kim, Young Pil Kim, Seog-Young Kim, Sang Ju LeeInvitrogen) were added, and the enzymatic reaction was stopped after 3 min by adding 50 µL of stop solution (DY994, R&D Systems). Absorbance was measured at 450 nm using a microplate reader to evaluate antibody binding

(6) Increased circulating heat shock protein Hsp70 serum levels as a potential biomarker in bronchial asthma patientsScientific ReportsNovember 18, 2025Ella Churyukina, Elena Oganesyan, Olga Ukhanova, Inga Kotieva, Marina Gulyan, Elena Koreeva, Ekaterina Portnyaga, Danila Bobkov, Stephanie E. Combs, Maxim Shevtsovtimes with wash buffer (cat. no. WA126, R&D Systems, USA). Then 300 µl of blocking buffer (cat. no. DY994, R&D Systems, USA) were added to each well and incubated for 2 h, followed by washing. The test sample

(7) Developing a therapeutic elastase that stimulates anti-tumor immunity by selectively killing cancer cellsCell Reports MedicineNovember 18, 2025Ravindra Gujar, Chang Cui, Maria Fumagalli, Nicole Martinez, Afshin Bahador, Alain Algazi, Kevin Harrington, Court Turner, Lev BeckerUltra TMB-ELISA,ThermoFisher Scientific,Cat# 34029 Stop Solution [2N Sμlfuric Acid],R&D Systems,Cat# DY994 Sodium carbonate,Sigma-Aldrich,Cat# 223530-500G Sodium bicarbonate,Sigma-Aldrich,Cat# S5761-500G TWEEN 20

(8) Multiplex Magnetic Biosensor Microarrays Facilitate Personalized Vaccination Schedules for SARS-CoV-2 VariantsBioChip JournalOctober 13, 2025Hyun-Kyung Um, Songeun Kim, Jueun Hwang, Yeeun Lee, Shan X. Wang, Jung-Rok Leehorseradish peroxide-conjugated streptavidin (SA-HRP; DY998), color substrates (DY999), and stop solution (DY994) were purchased from R&D Systems, and the absorbance signals were measured using a spectrophotometer

(9) Label‐Free Single‐Molecule ImmunoassayAdvanced ScienceJune 20, 2025Xiaoyan Zhou, Chao Chen, Shuang Zhou, Guangzhong Ma, Mohammad Javad H. N. Chemerkouh, Christine L. H. Snozek, Eric H. Yang, Jiapei Jiang, Brandyn Braswell, Zijian Wan, et al.conjugated horseradish peroxidase (streptavidin‐HRP, catalog no. DY998), stop solution (catalog no. DY994) and high‐binding microplate (catalog no. DY990) were purchased from R&D Systems. Gender unspecified

(10) Oncolytic VSV-IL-2 has enhanced anticancer vaccination adjuvant abilitiesJ Immunother CancerMay 19, 2025Victor Mullins-Dansereau, Marie-Lou Myre, Angelina Bardoul, Karen Geoffroy, Marco J Rallo Pita, Delphine Béland, Kim Leclerc Desaulniers, Dominic Guy Roy, Marie-Claude Bourgeois-DaigneaultBurlington, Massachusetts, USA). Finally, the chromogenic reaction was arrested using a stop solution (DY994 R&D Systems Minneapolis, Minnesota, USA), and signal was quantified by a SpectraMax multimode plate

(11) Impact of SARS-CoV-2 vaccination and of seasonal variations on the innate immune inflammatory responseFront ImmunolJanuary 14, 2025Hend Jarras, Isalie Blais, Benjamin Goyer, Wilfried W. Bazié, Henintsoa Rabezanahary, Mathieu Thériault, Kim Santerre, Marc-André Langlois, Jean-François Masson, Joelle N. Pelletier, et al.Cat. DY999) for 20 min at room temperature in the dark. The stop solution (2N H2SO4, R&D Systems, Cat. DY994) was added to the wells before reading at 450 and 540 nm with the SpectraMax 190 microplate reader (Molecular Devices

(12) Proteolytic shedding of CD46 from human hepatocytes indicates liver stressHeliyonDecember 15, 2024Kupke P, Yang Zhou J, Glehr G, Riquelme P, Scheibert L, Adenugba A, Schlitt HJ, Geissler EK, Werner JM, Hutchinson JA.substrates (DY999, R&D Systems) for 20 and 30 min, respectively. Finally, 50 μl/well stop solution (DY994, R&D Systems) is added without previous washing to terminate the reaction. The plate is then read using

(13) Distinct tumor architectures and microenvironments for the initiation of breast cancer metastasis in the brainCancer cellOctober 14, 2024Gan S, Macalinao DG, Shahoei SH, Tian L, Jin X, Basnet H, Bibby C, Muller JT, Atri P, Seffar E, et al.DYC002 Substrate Reagent Pack,R&D Systems,Cat #: DY999 "Stop Solution, 2N Sulfuric Acid",R&D Systems,Cat #: DY994 R428,MedChemExpress,Cat #: HY-15150 Critical commercial assays,Critical commercial assays,Critical

(14) Oncolytic vesicular stomatitis virus alone or in combination with JAK inhibitors is effective against ovarian cancerMolecular Therapy: OncologySeptember 19, 2024Karen Geoffroy, Victor Mullins-Dansereau, Kim Leclerc-Desaulniers, Mélissa Viens, Marie-Claude Bourgeois-Daigneaulta TMB/E substrate solution (ES022, Millipore Sigma, Burlington, MA) for 10 min and a stop solution (DY994, R&D Systems Inc). The absorbance was measured at 450 nm using an Ensight multimode plate reader (PerkinElmer)

(15) Alteration of Trophoblast Syncytialization by Plasmodium falciparum-Infected ErythrocytesMicroorganismsAugust 10, 2024López-Guzmán C, García AM, Vásquez AM.

(16) Immunoglobulin G N-glycan markers of accelerated biological aging during chronic HIV infectionNature communicationsApril 10, 2024Giron LB, Liu Q, Adeniji OS, Yin X, Kannan T, Ding J, Lu DY, Langan S, Zhang J, Azevedo JLLC, et al.

(17) Impact of Immunopathy and Coagulopathy on Multi-Organ Failure and Mortality in a Lethal Porcine Model of Controlled and Uncontrolled HemorrhageInternational journal of molecular sciencesFebruary 21, 2024Simovic MO, Bynum J, Liu B, Dalle Lucca JJ, Li Y.

(18) Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term CultureMethods and protocolsFebruary 15, 2024López-Guzmán C, García AM, Marín P, Vásquez AM.

(19) HDAC9‐mediated calmodulin deacetylation induces memory impairment in Alzheimer's diseaseCNS Neuroscience & TherapeuticsFebruary 7, 2024Hai‐Long Zhang, Shufen Hu, Pin Yang, Han‐Chun Long, Quan‐Hong Ma, Dong‐Min Yin, Guang‐Yin Xusubstrate solution for 20 min at room temperature, 50 μL of stop solution (2 N H2SO4, R&D Systems, DY994) was applied to each well. The optical density of each well was determined using a microplate reader

(20) APOE Christchurch-mimetic therapeutic antibody reduces APOE-mediated toxicity and tau phosphorylationAlzheimer's & dementia : the journal of the Alzheimer's AssociationFebruary 1, 2024Marino C, Perez-Corredor P, O'Hare M, Heuer A, Chmielewska N, Gordon H, Chandrahas AS, Gonzalez-Buendia L, Delgado-Tirado S, Doan TH, et al.detected spectroscopically as absorbance at 450 nm upon adding 2 N sulfuric acid (R&D Systems; Catalog No. DY994). Three independent experiments were conducted to ensure reproducibility. ELISA binding profiles were

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