cAMP Parameter Assay Kit KGE002B from R&D Systems, a Bio-Techne Brand

Product Specs
ItemcAMP Parameter Assay Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $470.00Supplier Page View Company Product Page
Catalog NumberKGE002B
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range3.75 - 240 pmol/mL
ReactivityMulti-Species
Sample TypeCell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (10 uL)
Sensitivity8.57 pmol/mL
Detection TargetcAMP
Assay Time3.5 hours
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeting NPSR1-mediated Hippo-YAP1 dysregulation suppresses gastric cancer progressionCellular and Molecular Life SciencesApril 9, 2026Wenjing Qin, Mei Ma, Weidan Fang, Xian Wang, Bin YucAMP levels were measured in accordance with the manufacturer’s protocol of cAMP Parameter Assay Kit (KGE002B, R&D Systems, Germany). Samples prepared and tested in triplicate. Statistical analysis All the data

(2) Type 2 diabetes risk alleles in peptidyl-glycine alpha-amidating monooxygenase influence GLP-1 levels and response to GLP-1 receptor agonistsGenome MedicineMarch 29, 2026Mahesh M. Umapathysivam, Elisa Araldi, Benoit Hastoy, Adem Y. Dawed, Hasan Vatandaslar, Johanna E. Mayrhofer, Peter Lindquist, Pamuditha N. Silva, Algera Goga, Geraldine O. Trüllinger, et al.cAMP parameter assay kit, a competitive enzyme immunoassay designed to measure cAMP (R&D Systems, #KGE002B). cAMP concentrations were further normalized to weight or protein content as measured by BCA assays. Total

(3) Ad-VT Oncolytic Adenovirus Suppresses Bladder Cancer via cAMP-Dependent AMPK-Raptor Activation and G2/M ArrestTumour Virus ResearchJanuary 29, 2026Dapeng Li, Jing Lu, Ran Zhu, Xianyan Sun, Cuiling Zhang, Mingzhe Sun, Chengyuan Ma, Chao Shang, Xiao Lipost-viral infection were assessed following the manufacturer's rotocol of the cAMP assay kit (R&D Systems, KGE002B). 4.12 Western blot analysis The cells were harvested, and total cellular proteins were extracted

(4) Identification and pharmacological characterization of a natural folipastatin as a CFTR inhibitor and potential anti-secretory agent in a human colonoid modelBMC Complementary Medicine and TherapiesDecember 4, 2025Saravut Satitsri, Rungtiwa Khumjiang, Nichakorn Worakajit, Bodee Nutho, Thanyada Rungrotmongkol, Patima Phainuphong, Taya Kitiyakara, Vatcharin Rukachaisirikul, Chatchai Muanprasatmeasurements Intracellular cAMP levels were evaluated using The Parameter cAMP Immunoassay Kit (catalog number KGE002B, R&D Systems, Minneapolis, MN, USA). Briefly, T84 cells were seeded (5 × 106 cells/well) onto the 24-well

(5) Functional characterization of neuropeptides that act as ligands for both calcitonin-type and pigment-dispersing factor-type receptors in a deuterostomeElifeNovember 21, 2025Xiao Cong, Huachen Liu, Lihua Liu, Nayeli Escudero Castelán, Kite GE Jones, Michaela Egertová, Maurice R Elphick, Muyan Chenluciferase reporter gene assay kit,MK,MF4001, commercial assay or kit,cAMP Assay kit,R&D systems,Cat No. KGE002B, Recombinant DNA reagent,pcDNA 3.1+vector with neomycin selectable marker (mammalian expression vector),YouBio,VT1001, Recombinant

(6) Functional characterization of neuropeptides that act as ligands for both calcitonin-type and pigment-dispersing factor-type receptors in a deuterostomeeLifeNovember 21, 2025Xiao Cong, Huachen Liu, Lihua Liu, Nayeli Escudero Castelán, Kite G E Jones, Michaela Egertová, Maurice R Elphick, Muyan Chenluciferase reporter gene assay kit,MK,MF4001, commercial assay or kit,cAMP Assay kit,R&D systems,Cat No. KGE002B, Recombinant DNA reagent,pcDNA 3.1+vector with neomycin selectable marker (mammalian expression vector),YouBio,VT1001, Recombinant

(7) Lactate promotes invasive Klebsiella pneumoniae liver abscess syndrome by increasing capsular polysaccharide biosynthesis via the PTS-CRP axisNature communicationsJuly 1, 2025Zhu J, Wang G, Xi W, Shen Z, Wei Q, Fang X, Li M.the supernatant was tested for cAMP levels by using cAMP Assay (USA R&D Systems, Inc. catalog number KGE002B) according to manufacturer’s recommendations. Statistics Statistical analysis was performed using

(8) HMGB1 Regulates Adipocyte Lipolysis via Caveolin-1 Signaling: Implications for Metabolic and Cardiovascular DiseasesInternational journal of molecular sciencesApril 29, 2025Hsu JC, Chiu KT, Chen CH, Wang CH, Shyue SK, Lee TS.

(9) An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic EffectSmall methodsMarch 1, 2025Mo X, Meng K, Li Z, Lan S, Ren Z, Fu X, Li C, Sun T, Xie D, Zhang Z, et al.by repeated freeze/thaw, and the supernatant was supplied with the cAMP Parameter Immunoassay Kit (KGE002B, R&D Systems, Minneapolis, USA) according to the manufacturer's instructions. Western Blotting RIPA

(10) A1, an innovative fluorinated CXCR4 inhibitor, redefines the therapeutic landscape in colorectal cancerCancer cell internationalJanuary 5, 2025Khorramdelazad H, Bagherzadeh K, Rahimi A, Darehkordi A, Najafi A, Karimi M, Khoshmirsafa M, Hassanshahi G, Safari E, Falak R.different concentrations of A1 and AMD3100 (10, 100, and 1000 nm/mL) using a cAMP ELISA kit (R&D Systems, KGE002B). Initially, CT-26 cells were seeded at a density of 106 × 5 cells per well and cultured for 24 h in

(11) Inhibition of CFTR-mediated intestinal chloride secretion by nornidulin: Cellular mechanisms and anti-secretory efficacy in human intestinal epithelial cells and human colonoidsPLOS ONEDecember 23, 2024Chamnan Yibcharoenporn, Thidarat Kongkaew, Nichakorn Worakajit, Rungtiwa Khumjiang, Praphatsorn Saetang, Saravut Satitsri, Vatcharin Rukachaisirikul, Chatchai MuanprasatcAMP measurements The intracellular cAMP level was measured by the cAMP Parameter Assay Kit (Cat# KGE002B, R&D Systems, Minneapolis, Minnesota, USA). T84 cells were plated onto 24-well plates at a density of

(12) Triglochin maritima Extracts Exert Anti-Melanogenic Properties via the CREB/MAPK Pathway in B16F10 CellsMarine drugsNovember 27, 2024Lee WH, Ha Y, Park JI, Joh WB, Park M, Kim JK, Jeon HK, Kim YJ.

(13) Dopamine receptor autoantibody signaling in infectious sequelae differentiates movement versus neuropsychiatric disordersJCI insightNovember 8, 2024Menendez CM, Zuccolo J, Swedo SE, Reim S, Richmand B, Ben-Pazi H, Kovoor A, Cunningham MW.

(14) Transcriptomic characterization of lung pericytes in systemic sclerosis-associated pulmonary fibrosisiScienceJune 21, 2024Ludivine Renaud, Carole L. Wilson, Robert Lafyatis, Lynn M. Schnapp, Carol A. Feghali-Bostwickand analyzed using the Parameter Competitive cAMP Assay (R&D Systems, Inc. Minneapolis, MN; catalog #KGE002B) according to the manufacturer’s instructions. The maximum limit of detection for this assay is 240

(15) Phosphoproteomics Reveals Selective Regulation of Signaling Pathways by Lysophosphatidic Acid Species in MacrophagesCellsMay 9, 2024Dietze R, Szymanski W, Ojasalu K, Finkernagel F, Nist A, Stiewe T, Graumann J, Müller R.quantitatively determine cyclic AMP (cAMP) levels in cell lysate, we utilized the cAMP Parameter Assay Kit (KGE002B, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s protocol. Prior to stimulation with

(16) Comparative analysis of SEC61A1 mutant R236C in two patient-derived cellular platformsScientific reportsApril 25, 2024Weiand M, Sandfort V, Nadzemova O, Schierwagen R, Trebicka J, Schlevogt B, Kabar I, Schmidt H, Zibert A.

(17) Control of lipolysis by a population of oxytocinergic sympathetic neuronsNatureJanuary 1, 2024Li E, Wang L, Wang D, Chi J, Lin Z, Smith GI, Klein S, Cohen P, Rosen ED.minutes. cAMP levels were measured from cell lysates using the cAMP Parameter Assay Kit (R&D systems, KGE002B). Cold tolerance test Mice were acclimatized at thermoneutrality (30°C) for four weeks and then shifted

(18) Destruction of self-derived PAMP via T3SS2 effector VopY to subvert PAMP-triggered immunity mediates Vibrio parahaemolyticus pathogenicityCell reportsOctober 31, 2023Wu X, Zhou L, Ye C, Zha Z, Li C, Feng C, Zhang Y, Jin Q, Pan J.

(19) A Side-by-Side Comparison of Wildtype and Variant Melanocortin 1 Receptor Signaling with Emphasis on Protection against Oxidative Damage to DNAInternational journal of molecular sciencesSeptember 21, 2023Cerdido S, Sánchez-Beltrán J, Lambertos A, Abrisqueta M, Padilla L, Herraiz C, Olivares C, Jiménez-Cervantes C, García-Borrón JC.cAMP was measured with a commercial competitive enzyme immunoassay from R&D Systems (catalog number KGE002B). Parallel dishes were used for protein determination with bicinchoninic acid. To estimate ERK activation,

(20) A Putative Role of Vasopressin/Oxytocin-Type Neuropeptide in Osmoregulation and Feeding Inhibition of Apostichopus japonicusInternational Journal of Molecular SciencesSeptember 20, 2023Xiao Cong, Huachen Liu, Yingqiu Zheng, Muyan Chenmin after starvation for two hours, and then detected using the cAMP Assay kit (R&D systems, Cat No. KGE002B). For the intracellular calcium measurement assay, HEK293T cells transfected with AjHOR/pcDNA 3.1(+)

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cAMP ELISA

cAMP ELISA

cAMP ELISA

cAMP ELISA

cAMP Parameter Assay Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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cAMP Parameter Assay Kit from R&D Systems, a Bio-Techne Brand

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