Human TNF-alpha DuoSet ELISA DY210-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman TNF-alpha DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $450.00Supplier Page View Company Product Page
Catalog NumberDY210-05
Quantity1 Kit (for 5 Plates) (also 1 Kit (for 15 Plates))
ApplicationsELISA
Detection Range15.60 - 1000 pg/mL
ReactivityHuman
Detection TargetTNF-alpha
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Bioengineered iPSC-derived human macrophages with increased angiotensin-converting enzyme (ACE) expression suppress solid tumor growthSignal Transduct Target TherApril 13, 2026Tomohiro Shibata, Shabir Bhat, DuoYao Cao, Suguru Saito, Noor Khan, Ellen A. Bernstein, Takeshi Tokudome, Derick Okwan-Duodu, Warren G. Tourtellotte, Kenneth E. Bernstein, et al.commercial solid-phase ELISA kit protocol to measure IL-1β (Invitrogen, BMS224-2), TNFα (R&D Systems, DY210-05), IL-6 (R&D Systems, DY206-05), IL-8 (R&D Systems, DY208-05), IL-4 (R&D Systems, DY204-05), IL-10 (R&D Systems

(2) Cadmium Toxicity Effects on Histone Modifiers, Enzyme Activity and Adipokines in Human Adipose Tissue CellsMoleculesMarch 23, 2026Victor Tadeu Gonçalves Plata, Júlia Fernandes Barcella, Raphael Justa Saran, Artur Francisco da Silva Neto, Yasmin Alaby Martins Ferreira, Andressa Bolsoni-Lopes, Lila Missae Oyama, Lucia Maria Armelin-Correa, Maria Isabel Cardoso Alonso-ValeELISA kits (Quantikine M; R&D Systems, Minneapolis, MN, USA; Catalog numbers DY279-05 (MCP1/CCL2), DY210-05 (TNFA), and DY1065-05 (ADIPOQ), respectively. 4.8. Enzyme Activity Assay Enzymatic assays for CAT

(3) Toll-like receptor signaling outcome is determined by the stoichiometry of the endogenous TRIFosomeSci AdvMarch 6, 2026Martin C. Moncrieffe, Prasanna Suresh, Joe Boyle, Yuhao Cui, Bharti Nawalpuri, Brett Verstak, Yu P. Zhang, Ziwei Zhang, Marcus Taylor, Edward H. Egelman, et al.to the manufacturers’ instructions. TNFα levels were quantified using the Human TNFα DuoSet ELISA (DY210-05, R&D Systems), and IFN-β levels were measured using the VeriKine-HS Human Interferon Beta Serum ELISA Kit

(4) Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral KeratinocytesBioFactorsFebruary 18, 2026Yosuke Shikama, Kayo Yoshida, Yuka ShikamaBioLegend), Human Lipocalin‐2/NGAL DuoSet ELISA (#DY1757, R&D Systems), and Human TNF‐alpha DuoSet ELISA (#DY210‐05, R&D Systems), respectively. Optical density at 450 nm was measured using a microplate reader (#51119050,

(5) FOXC1 Regulates Cytokine Signaling, Inflammatory Pathways, and Retinoid Metabolism to Maintain Limbal Epithelial Cell Homeostasis In VitroInternational Journal of Molecular SciencesFebruary 15, 2026Swarnali Kundu, Maryam Amini, Tanja Stachon, Fabian Norbert Fries, Berthold Seitz, Zhen Li, Shuailin Li, Shanhe Liu, Shao-Lun Hsu, Shweta Suiwal, et al.pg/mL IL-6,DY206-05/P437281,600 pg/mL–4.7 pg/mL IL-8,DY208-05/P445124,2000 pg/mL–15.65 pg/mL TNF-α,DY210-05/P452568,1000 pg/mL–7.8 pg/mL TGF-β,DY240-05/P424503,2000 pg/mL–15.65 pg/mL VEGFA,DY293B-05/P394527,2000

(6) Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signalingPharmaceutical BiologyDecember 31, 2025Lan-Chi Hsieh, Shu-Ling Hsieh, Tsu-Ni Ping, Yi-Chun Huang, Ssu-Jung Lin, Hsing-Yu Chi, Chih-Chung Wu(R&D, DY206-05), human total IL-18 DuoSet ELISA (R&D, DY318-05), or human TNF-α DuoSet ELISA (R&D, DY210-05) kits (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturers’ instructions. This study

(7) Signatures of Trained Immunity Following mRNA Vaccination: Differences Between mRNA-1273 and BNT162b2Journal of Clinical ImmunologyDecember 24, 2025Martin Kunc, Bradley Joseph Whitehead, Lars Jørgen Østergaard, Martin Tolstrup, Christine Stabell Benn, Christian Morberg Wejse, Peter Nejsuminstructions. The cytokines measured included IL-6 (cat# DY206-05), IL-10 (cat# DY217B-05), TNFα (cat# DY210-05) and IFNγ (cat# DY285B-05). The chemokines measured included CCL2 (cat# DY279-05) and CXCL1 (cat# DY275-05). SARS-CoV-2

(8) Variable steady inflammation and inflammatory responses in precision-cut lung slices from various IPF lung RegionsRespiratory ResearchNovember 28, 2025Carlos Machahua, Thomas M. Marti, Namrata Kewalramani, Patrick Dorn, Lavinia Neubert, Christina Petzold-Mügge, Manuela Funke-Chambourmeasurements Supernatants from the experiments were analysed for human tumour necrosis factor (TNF) (#DY210-05, R&D System, USA), human IL-6 (#DY206-05, R&D System), and human IL-8 levels (#DY208-05, R&D Systems)

(9) Amino acid-equipped poly(ester amide) nanoparticles for improved encapsulation efficiency of lipophilic anti-inflammatory 6BIGOE based on molecular dynamic simulationsInternational journal of pharmaceuticsNovember 10, 2025Westhoff J, Weber C, Bachmann V, Göppert NE, Peric M, George A, Sierka M, Hoeppener S, Vilotijevic I, Schubert US, et al.

(10) Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigenThe FEBS JournalNovember 1, 2025Saitong Muneekaew, Pasut Sasithong, Koollawat Chupradit, Kritayaporn Saiprayong, Thunchanok Nuchphongsai, Methichit Wattanapanitchdebris. Tumor necrosis factor‐alpha (TNF‐α) was examined using the DuoSet® ELISA kit (R&D Systems, DY210‐05) according to the manufacturer's protocol. The absorbance was measured using the spectrophotometer (BioTek

(11) Zn-doped CaP coating equips Ti implants with corrosion resistance, biomineralization, antibacterial and immunotolerant activitiesJournal of advanced researchNovember 1, 2025Parau AC, Büyüksungur S, Li G, Liu Q, Badillo E, Blum L, Schmidt J, Pana I, Vitelaru C, Marinescu IM, et al.

(12) Platelets induce epithelial to mesenchymal transition in renal proximal tubular epithelial cells through TGF-β signaling pathwayMol MedOctober 29, 2025Ukhti Jamil Rustiasari, Melissa Uil, Xiaomeng Zhang, Nike Claessen, Loes Butter, Sandrine Florquin, Alessandra Tammaro, Joris J.T.H. Roelofscytokine levels were measured using ELISA kits from R&D Systems: human IL-1β (#DY201-05), human TNF-α (#DY210-05), and human IL-10 (#DY217B-05). Platelet factor 4 (PF4) was measured using a combination of reagents

(13) Engineering and in vitro evaluation of semi-dissolving, hydrogel-forming polymeric microneedles for sustained-release drug deliveryInternational journal of pharmaceuticsSeptember 15, 2025Abdelghany TM, Vo N, Vukajlovic D, Smith E, Wong JZ, Jackson E, Hilkens CMU, Lau WM, Ng KW, Novakovic K.

(14) Deciphering cross-cohort metabolic signatures of immune responses and their implications for disease pathogenesisMolecular Systems BiologySeptember 10, 2025Jianbo Fu, Nienke van Unen, Andrei Sarlea, Nhan Nguyen, Martin Jaeger, Javier Botey Bataller, Valerie A C M Koeken, L Charlotte de Bree, Vera P Mourits, Simone J C F M Moorlag, et al.DY201-05 IL-6 DuoSet ELISA kit,R&D Systems,Cat# DY206-05 TNF-α DuoSet ELISA kit,R&D Systems,Cat# DY210-05 IFN-γ ELISA kit,Sanquin,Cat# 75-0528-46 Software,Software,Software R,R Foundation for Statistical

(15) Estrogen induces the alternative activation of macrophages through binding to estrogen receptor-alphaExperimental and molecular pathologySeptember 1, 2025Belboul A, Ashworth J, Fadel A, Mcloughlin J, Mahmoud A, El Mohtadi M.

(16) Engineering a 3D wounded skin equivalent to study early inflammatory and regenerative responses in vitroFrontiers in Bioengineering and BiotechnologyAugust 22, 2025Rima Nuwayhid, Nguyen Ngoc-Huyen, Dmitry Notov, Stefan Langer, Olga Kurow(Minneapolis, MN, United States): Human IL-1α/IL-1F1 (DY200-05), Human IL-6 (DY206-05), Human TNF-α (DY210-05), Human IL-8/CXCL8 (DY208-05), and Human IL-33 (DY3625B-05), according to the manufacturer’s instructions. 2.5

(17) The effect of travoprost on primary human limbal epithelial cells and the siRNA-based aniridia limbal epithelial cell model, in vitroPLoS OneAugust 19, 2025Shuailin Li, Tanja Stachon, Shanhe Liu, Zhen Li, Shao-Lun Hsu, Swarnali Kundu, Fabian N. Fries, Berthold Seitz, Maryam Amini, Shweta Suiwal, et al.Human MMP-9 DuoSet ELISA,DY911,"R&D Systems, Bio-Techne, Minneapolis, USA" Human TNF-alpha DuoSet ELISA,DY210−05,"R&D Systems, Bio-Techne, Minneapolis, USA" ELISA kits used in the study.

(18) Houttuynia cordata Exhibits Anti-Inflammatory Activity Against Interleukin-1β-Induced Inflammation in Human Gingival Epithelial Cells: An In Vitro StudyDentistry journalAugust 7, 2025Kunimatsu R, Ikeoka S, Koizumi Y, Odo A, Tanabe I, Kawashima Y, Kiso A, Hashii Y, Tsuka Y, Tanimoto K.

(19) Can Biomarkers Predict Kidney Function Recovery and Mortality in Patients with Critical COVID-19 and Acute Kidney Injury?Diagnostics (Basel)August 5, 2025Noemí Del Toro-Cisneros, José C. Páez-Franco, Miguel A. Martínez-Rojas, Isaac González-Soria, Juan Antonio Ortega-Trejo, Hilda Sánchez-Vidal, Norma A. Bobadilla, Alfredo Ulloa-Aguirre, Olynka Vega-VegaTNF-α were analyzed with commercially available ELISA kits (DY206-05 for IL-6, DY217B-05 for IL-10, and DY210-05 for TNF-α; R&D Systems), following the manufacturer’s instructions. 2.2. Gas Chromatography/Mass Spectrometry

(20) Secretion of a VEGF-Blocking scFv Enhances CAR T-cell PotencyCancer Immunology ResearchAugust 1, 2025Valentina M. Supper, Hannah Donner, Filippo Birocchi, Alexandra Bratt, Giulia Escobar, Michael C. Kann, Sangwoo Park, Grace Martin, Felix Korell, Hana Takei, et al.T cells on day 13 of production. ELISA’s for Granzyme B (R&D Systems DY2906–05), TNF-α (R&D Systems DY210–05), IFN-γ (R&D Systems DY285B), and IL2 (R&D Systems, DY202) were performed according to the manufacturer’s

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TNF-alpha ELISA

Human TNF-alpha DuoSet ELISA

Human TNF-alpha DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human TNF-alpha DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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