Human IL-6 DuoSet ELISA DY206-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IL-6 DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $450.00Supplier Page View Company Product Page
Catalog NumberDY206-05
Quantity1 Kit (for 5 Plates) (also 1 Kit (for 15 Plates))
ApplicationsELISA
Detection Range9.38 - 600 pg/mL
ReactivityHuman
Detection TargetIL-6
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterleukin 6
NCBI Gene AliasesBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Bioengineered iPSC-derived human macrophages with increased angiotensin-converting enzyme (ACE) expression suppress solid tumor growthSignal Transduct Target TherApril 13, 2026Tomohiro Shibata, Shabir Bhat, DuoYao Cao, Suguru Saito, Noor Khan, Ellen A. Bernstein, Takeshi Tokudome, Derick Okwan-Duodu, Warren G. Tourtellotte, Kenneth E. Bernstein, et al.protocol to measure IL-1β (Invitrogen, BMS224-2), TNFα (R&D Systems, DY210-05), IL-6 (R&D Systems, DY206-05), IL-8 (R&D Systems, DY208-05), IL-4 (R&D Systems, DY204-05), IL-10 (R&D Systems, DY217B-05), IL-13

(2) Neuron-derived mitochondrial DNA (mtDNA) activates microglia via the Z-DNA binding protein 1 (ZBP1)-mediated pathway in mild traumatic brain injuryProc Natl Acad Sci U S AApril 7, 2026Michela Marcatti, Javier Allende Labastida, Tony Zifeng Tang, Akbar Ahmad, Christina Payne, Nora Schwartz, Paula Villarreal, Olivia D. Solomon, Gracie Vargas, Ping Wu, et al.treatment, the amount of IL-6 and TNFα in the culture medium was measured using DuoSet Kits (R&D Systems #DY206-05) according to the manufacturer’s recommendations. Proximity Ligation Assay. HMC3 microglia were seeded

(3) Tissue and Isoform-Specific Effects of Platelet-Derived Growth Factor on Neonatal-Derived Dermal and Fetal-Derived Lung Fibroblast Profibrotic FunctionsCellsApril 1, 2026Brandon Kohlen, Raveen Badyal, Kevin J. Keen, James V. Dunne, Tillie-Louise HackettEnzyme-Linked Immunosorbent Assay (ELISA) Cell-free supernatant was used to assess the release of IL-6 (cat. DY206-05), IL-8 (cat. DY208), and IL-11 (cat. DY218) using DuoSet ELISAs, manufactured by R&D Systems (Minneapolis,

(4) Tumor growth inhibitory activity of the P2X7 receptor antagonist AZ10606120 in two cell lines of human glioblastomaPurinergic SignalMarch 28, 2026Michela Graziano, Silvia Bono, Michela Pizzoferrato, Chiara Ferraro, Flavia Grossi, Pierluigi Navarra, Maria Martire, Lucia Lisithe culture media were assayed by enzyme-linked immunosorbent assay (Human IL-6 DuoSet ELISA, Cat No: DY206-05, R&D Systems). Cells were plated at a density of 10,000 cells/well in 96-well plates. The day before

(5) The diagnostic and immunomodulatory role of IL-37 in pediatric sepsisFrontiers in ImmunologyFebruary 12, 2026Jin Ma, Yanling Zhang, Changrui Sun, Hao Yang, Zhixin Song, Feiqi Huang, Chunxiang Wuconcentrations of IL-37 (Catalog No: DY1975-05, Lot No: P325850, R&D Systems, USA) and IL-6 (Catalog No: DY206-05, Lot No: P326914, R&D Systems, USA) in pediatric sepsis patients were measured using ELISA. 2.3 Animal models

(6) Effect of Bifidobacterium longum on cognition and microbiota in post-stroke patients: a single-blinded, controlled trialInternational Journal of Medical SciencesFebruary 11, 2026Shu-Ting Lin, Te-Hsuan Tung, Yen-Nung Lin, Feng-Hang Chang, Yu Zhi Lian, Chien-Hung Lai, Tsan-Hon Liou, Chi-Chang Huang, Ya-Ling Chen, Jane C.-J. Chaosuch as tumor necrosis factor-α (TNF-α) (430204, BioLegend, San Diego, CA, USA), interleukin-6 (IL-6) (DY206-05, R&D Systems, Inc., Minneapolis, MN, USA), and IL-10 (430607, BioLegend, San Diego, CA, USA) were measured

(7) Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory ProbioticsInternational Journal of Molecular SciencesDecember 27, 2025Yin-Ling Chiang, Men-Yee Chiew, Sheng-Jye Lim, Ding-Li Chou, Huai-En Lu, Ching-Ping TsengThe culture supernatants were analyzed using commercially available ELISA kits to measure IL-6 (R&D, #DY206-05), IL-10 (R&D Systems, #DY217B-05), and IL-12p40 (R&D Systems, #DY1240-05), following the manufacturer’s

(8) Signatures of Trained Immunity Following mRNA Vaccination: Differences Between mRNA-1273 and BNT162b2Journal of Clinical ImmunologyDecember 24, 2025Martin Kunc, Bradley Joseph Whitehead, Lars Jørgen Østergaard, Martin Tolstrup, Christine Stabell Benn, Christian Morberg Wejse, Peter Nejsum(DuoSet, R&D systems) according to manufacturer instructions. The cytokines measured included IL-6 (cat# DY206-05), IL-10 (cat# DY217B-05), TNFα (cat# DY210-05) and IFNγ (cat# DY285B-05). The chemokines measured included

(9) Intracellular C3 regulates the immune response to infection via NF-κB signalingCell Mol Life SciDecember 24, 2025Katarzyna Kuska, Serena Bettoni, Frida Mohlin, Maja Chrobak, Vaishnavi Dandavate, Saleh Moradi, Ben King, Kristian Riesbeck, Anna M. BlomBio-Plex Pro human cytokine 27-plex assay (Bio-Rad #M500KCAF0Y) or commercial ELISAs from R&D systems (Human IL-6 DuoSet ELISA # DY206-05, Human IL-8 DuoSet ELISA # DY208, Human CCL2/MCP-1 DuoSet ELISA #DY279, Human CCL5/RANTES DuoSet ELISA

(10) Pervasive food contaminant ochratoxin‐A induces energy crisis: Mitochondrial dysfunction in human primary proximal tubule cellsToxicology ReportsDecember 1, 2025Anish Mahadeo, Theo K. Bammler, James MacDonald, Angela R. Zheng, Catherine K. Yeung, Jonathan Himmelfarb, Edward J. Kelly(IL-6) and C-X-C motif chemokine ligand 6 (CXCL6) ELISA kits were acquired from R&D systems (Catalog # DY206–05 and DY333, respectively) and experiments were conducted following manufacturer’s protocol. Dilution

(11) Variable steady inflammation and inflammatory responses in precision-cut lung slices from various IPF lung RegionsRespiratory ResearchNovember 28, 2025Carlos Machahua, Thomas M. Marti, Namrata Kewalramani, Patrick Dorn, Lavinia Neubert, Christina Petzold-Mügge, Manuela Funke-Chambourexperiments were analysed for human tumour necrosis factor (TNF) (#DY210-05, R&D System, USA), human IL-6 (#DY206-05, R&D System), and human IL-8 levels (#DY208-05, R&D Systems) by ELISA. Samples were pre-diluted and

(12) Telomerase modRNA Offers a Novel RNA-Based Approach to Treat Human Pulmonary FibrosisAging cellNovember 1, 2025Ye JL, Grieger K, Lu D, Brandenberger C, Juchem M, Jordan M, Oehlsen L, Zardo P, Werlein C, Hesse C, et al.

(13) TRIM16 mediates secretory autophagy in head and neck cancer-associated fibroblastsAutophagyNovember 1, 2025Ly T, Pickard B, Pandey A, Yap M, Opara J, Arnold L, Martinez-Rivera N, Rosa-Molinar E, New J, Werner L, et al.quantifying the total expression of proteins. ELISA The human IL6 sandwich ELISA (R&D Systems, DY008 and DY206–05) was used according to the manufacturer’s instructions. CAFs (70% confluent in 6-cm plate) were transfected

(14) Zn-doped CaP coating equips Ti implants with corrosion resistance, biomineralization, antibacterial and immunotolerant activitiesJournal of advanced researchNovember 1, 2025Parau AC, Büyüksungur S, Li G, Liu Q, Badillo E, Blum L, Schmidt J, Pana I, Vitelaru C, Marinescu IM, et al.

(15) Investigating the Impact of the Secretome From hAMSCs on HT-29 Colon Cancer Cells via TNF-α/TGF-β/c-MYC Signaling Pathways Using a Three-Dimensional Cell Culture ModelImmun Inflamm DisOctober 15, 2025Mohammad Ali Majdoddin, Fatemeh Safarimembrane. 2.5 ELISA ELISA was utilized to assess the levels of IL‐4 (DY204‐05), IL‐8 (DY208‐05), and IL‐6 (DY206‐05) in the supernatant of HT‐29 cancer cells treated with secretome for 48 h. ELISA kits from R&D Systems,

(16) Deciphering cross-cohort metabolic signatures of immune responses and their implications for disease pathogenesisMolecular Systems BiologySeptember 10, 2025Jianbo Fu, Nienke van Unen, Andrei Sarlea, Nhan Nguyen, Martin Jaeger, Javier Botey Bataller, Valerie A C M Koeken, L Charlotte de Bree, Vera P Mourits, Simone J C F M Moorlag, et al.85187-10-6 IL-1β DuoSet ELISA kit,R&D Systems,Cat# DY201-05 IL-6 DuoSet ELISA kit,R&D Systems,Cat# DY206-05 TNF-α DuoSet ELISA kit,R&D Systems,Cat# DY210-05 IFN-γ ELISA kit,Sanquin,Cat# 75-0528-46 Software,Software,Software R,R

(17) Evaluation of toxicological mechanisms of ochratoxin-A in human primary proximal tubule epithelial cellsbioRxiv : the preprint server for biologySeptember 4, 2025Mahadeo A, Bammler TK, MacDonald J, Zheng AR, Yeung CK, Himmelfarb J, Kelly EJ.

(18) Engineering a 3D wounded skin equivalent to study early inflammatory and regenerative responses in vitroFrontiers in Bioengineering and BiotechnologyAugust 22, 2025Rima Nuwayhid, Nguyen Ngoc-Huyen, Dmitry Notov, Stefan Langer, Olga Kurowkits from R&D Systems (Minneapolis, MN, United States): Human IL-1α/IL-1F1 (DY200-05), Human IL-6 (DY206-05), Human TNF-α (DY210-05), Human IL-8/CXCL8 (DY208-05), and Human IL-33 (DY3625B-05), according to the

(19) Myokine Circulating Levels in Postmenopausal Women with Overweight or Obesity: Effects of Resistance Training and/or DHA-Rich n-3 PUFA SupplementationNutrientsAugust 5, 2025Martínez-Gayo A, Félix-Soriano E, Ibáñez-Santos J, García-Unciti M, González-Muniesa P, Moreno-Aliaga MJ, On Behalf Of Obelex Project.their respective manufacturers. IL-6, METRNL, and irisin were analyzed using Human IL-6 DuoSet® ELISA #DY206-05, Human Meteorin-like/METRNL DuoSet® ELISA #DY7867-05, and Human Irisin/FNDC5 DuoSet® ELISA #DY9420-05,

(20) Can Biomarkers Predict Kidney Function Recovery and Mortality in Patients with Critical COVID-19 and Acute Kidney Injury?Diagnostics (Basel)August 5, 2025Noemí Del Toro-Cisneros, José C. Páez-Franco, Miguel A. Martínez-Rojas, Isaac González-Soria, Juan Antonio Ortega-Trejo, Hilda Sánchez-Vidal, Norma A. Bobadilla, Alfredo Ulloa-Aguirre, Olynka Vega-VegaSerum concentrations of IL-6, IL-10, and TNF-α were analyzed with commercially available ELISA kits (DY206-05 for IL-6, DY217B-05 for IL-10, and DY210-05 for TNF-α; R&D Systems), following the manufacturer’s instructions. 2.2.

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IL-6 ELISA

Human IL-6 DuoSet ELISA

Human IL-6 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human IL-6 DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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