Description
This anti-Tg autoantibody IgG ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human anti-Tg IgG autoantibody level in test sample. Assay calibrators, controls and pre-diluted human serum samples containing anti-Tg IgG autoantibody are added to microtiter wells of microplate that was coated with high affinity streptavidin on its wall. The autoantibody reaction will not start until the addition of a biotinylated human Tg Antigens. After the first incubation period, the unbound protein matrix is removed in the subsequent washing step. A horseradish peroxidase-conjugated rabbit anti-human IgG subclass specific antibody (tracer antibody) is added to each well