PathScan ® Total IRS-1 Sandwich ELISA Kit from Cell Signaling Technology

CST's PathScan® Total IRS-1 Sandwich ELISA Kit #7328 detects endogenous levels of IRS-1. As shown in Figure 1, a significant induction of IRS-1 phosphorylation at Ser612 can be detected in hSkMC and CHO (IR/IRS-1) cells following treatment with insulin using the Phospho-IRS-1 (Ser612) Sandwich ELISA Kit #7332. However, the level of total IRS-1 (phospho and nonphospho) remains unchanged as shown by Western analysis and by PathScan® Total IRS-1 Sandwich ELISA Kit #7328 (Figure 1). In Figure 3, Western analysis of protein captured in microwells coated with the IRS-1 antibody shows a major band corresponding to the IRS-1 protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species