CF®640R TUNEL Assay Apoptosis Detection Kit 30074 from Biotium, Inc.

Product Specs
ItemCF®640R TUNEL Assay Apoptosis Detection Kit
CompanyBiotium, Inc.
Price $587.00Supplier Page View Company Product Page
Catalog Number30074
Source/Expression SystemBSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; TdT enzyme (calf thymus; recombinant, produced in E. coli)
StorageProtect from light;

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Biotium, Inc.

46117 Landing Parkway

Fremont, CA 94538

United States

Phone: 510-265-1027
510-265-1352

Email: order@biotium.com

Company Profile

Website: https://biotium.com/company/

(1) Aldh3a1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to amyotrophic lateral sclerosis progressionFree Radical Biology and MedicineJune 1, 2026Ang Li, Li Dong, Xuejun Li, Jianxun Yi, Jo-Yu Wu, Jianjie Ma, Jingsong Zhoumyotubes were cultured for another 16 hours in regular low serum medium before TUNEL assay (Biotium 30074) or LDH leakage-based cytotoxicity assay (Promega G1780). This is because apoptosis can take 12-24 hours

(2) Longitudinal in vivo imaging of dynamic immune–vascular responses to meningeal cerebrovascular injuryJournal of NeuroinflammationMarch 17, 2026Aurora Kraus, Jean S. Prosper-Santiago, Aleksandra Potapova, John Prevedel, Daniel Castranova, Brant M. WeinsteinPBST and stained with TUNEL using kit instructions (TUNEL Assay Apoptosis Detection Kit, Biotium CAT #30074). Samples were incubated with TUNEL Reaction mix, which included 2.5 µL of TdT Enzyme to 50µL of TUNEL

(3) Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth MovementBiology (Basel)January 19, 2026Yohei Morihana, Masato Nakagawa, Yue Zhou, Hidetoshi Morikuni, Zi Deng, Yoshitomo Honda, Aki NishiuraCell death was analyzed by TUNEL staining using a CF® Dye TUNEL Assay Apoptosis Detection Kit (Cat. #30074; Biotium, Fremont, CA, USA). Fluorescence images were obtained using an all-in-one fluorescence microscope

(4) Cryptococcus exploits delayed microglial activation, and microglial osteopontin (OPN/Spp1) impairs peripheral host controlCell Host & MicrobeDecember 10, 2025Estefany Y. Reyes, Jae Yong, Devon T. DiPalma, Jonathan L. Messerschmidt, Miranda Lumbreras, Hana H. Hendi, Danira R. Mukhamedyarova, Emily C. Troutman, Emily J. Wert, Mari L. Shinoharacommercial assays,Critical commercial assays CF640R TUNEL Assay Apoptosis Detection Kit,Biotium,Cat# 30074 TACS Nuclease,R&D,Cat# 4800-30-N Papain Dissociation System,Worthington Biochemical Corporation,Cat#

(5) Post-embryonic tail development through molting of the freshwater shrimp Neocaridina denticulataiScienceFebruary 21, 2025Haruhiko Adachi, Nobuko Moritoki, Tomoko Shindo, Kazuharu Arakawa,Thermo Fisher Scientific,W32466 UV-LED resin,Padico,403242 CF640R TUNEL Assay Apoptosis Detection Kit,Biotium,30074 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ,Schneider et

(6) ALDH3A1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to denervation and Amyotrophic Lateral Sclerosis progressionbioRxivDecember 7, 2024Ang Li, Li Dong, Xuejun Li, Jianxun Yi, Jianjie Ma, Jingsong Zhoumyotubes were cultured for another 16 hours in regular low serum medium before TUNEL assay (Biotium 30074) or LDH leakage-based cytotoxicity assay (Promega G1780). This is because apoptosis can take 12–24 hours

(7) Topical application of synthetic melanin promotes tissue repairnpj Regenerative MedicineNovember 2, 2023Dauren Biyashev, Zofia E. Siwicka, Ummiye V. Onay, Michael Demczuk, Dan Xu, Madison K. Ernst, Spencer T. Evans, Cuong V. Nguyen, Florencia A. Son, Navjit K. Paul, et al.The TUNEL assay was performed using the CF®640 R Dye TUNEL assay apoptosis detection kits (Biotium, 30074) according to the manufacturer’s directions and visualized with an ECLIPSE Ti2 inverted microscope (Nikon

(8) Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametesCell ReportsOctober 11, 2023Neha Gupta, Cristina Soriano-Úbeda, Paula Stein, Virginia Savy, Brian N. Papas, Goli Ardestani, Ingrid Carvacho, Dominique Alfandari, Carmen J. Williams, Rafael A. Fissorefor 30 min. For the detection of apoptotic cells, we used the TUNEL Assay Apoptosis Detection Kit (30074, Biotium) following the manufacturer’s protocol and instructions. Briefly, fixed and permeabilized BLs

(9) RANKL(+) senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolyticsInternational journal of oral scienceMay 30, 2023Zhou Y, Nishiura A, Morikuni H, Deng W, Tsujibayashi T, Momota Y, Azetsu Y, Takami M, Honda Y, Matsumoto N.TUNEL staining reagents were obtained from the CF™ Dye TUNEL Assay Kits ALEXA FLUOR® 647 (Cat. No. 30074, Fermont, CA, Biotium, Inc.). All sections were stained according to the protocol in kit and mounted

(10) Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiencyCommunications BiologyDecember 1, 2022Bryan J. González et al.3% donkey serum with 0.1% triton X-100 diluted in PBS overnight at 4 °C. For tunel assay (Biotium, 30074) cells were incubated with fluorescent biotinylated nucleotide conjugate according to the manufacturer’s

(11) Lacosamide decreases neonatal seizures without increasing apoptosisEpilepsiaDecember 1, 2022Langton RL, Sharma S, Tiarks GC, Bassuk AG, Glykys J.were labeled with deoxyuridine triphosphate conjugated to a photostable far‐red fluorescent dye (Cat‐30074, Biotium) using the TUNEL method according to Biotium's commercial protocol. We used a TUNEL+ control

(12) "Disrupting autorepression circuitry generates ""open-loop lethality"" to yield escape-resistant antiviral agents"CellJune 9, 2022Chaturvedi S, Pablo M, Wolf M, Rosas-Rivera D, Calia G, Kumar AJ, Vardi N, Du K, Glazier J, Ke R, et al.Detection Kit, Biolegend, Cat # 640932) or by using the TUNEL Assay Apoptosis Detection kit (Biotium, Cat #30074) according to the manufacturer’s instructions. TLR9 expression analysis was performed on ARPE-19 cells

(13) The AIM2 inflammasome is activated in astrocytes during the late phase of EAEJCI InsightApril 22, 2022William E. Barclay, Nupur Aggarwal, M. Elizabeth Deerhake, Makoto Inoue, Toshiaki Nonaka, Kengo Nozaki, Nathan A. Luzum, Edward A. Miao, Mari L. Shinoharathick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, catalog 30074). Antibodies used for staining are indicated in Supplemental Table 1. Preparation of tissue slice culture

(14) Secreted osteopontin from CD4⁺ T cells limits acute graft-versus-host diseaseCell reportsDecember 28, 2021Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML.antibodies (listed in Key resources Table) and CF640R TUNEL (640R TUNEL Assay Apoptosis Detection Kit; 30074, Biotium) for immunofluorescence analysis. Intenstinal permeability assay using FITC-Dextran Mice

(15) Efferocytosis by Paneth cells within the intestineCurrent biology : CBJune 7, 2021Shankman LS, Fleury ST, Evans WB, Penberthy KK, Arandjelovic S, Blumberg RS, Agaisse H, Ravichandran KS.staining. One set of enteroids underwent apoptosis detection using the CF640R TUNEL Assay (Biotium #30074) allowing the reagent to sit for 2 hours at 37°C before EDU detection (GeneCopoeia #A003) for 30min.

(16) Potential circadian effects on translational failure for neuroprotectionNatureJune 18, 2020Elga Esposito et al.BSA blocking for 1 hr at room temperature. The slides were then transferred to the TUNEL (Biotium, 30074) working solution for 1 hr at 37 °C and then rinsed. To combine with Fluoro-Jade, the slides were then

(17) Irradiation abolishes smooth muscle investment into vascular lesions in specific vascular bedsJCI InsightAugust 9, 2018Alexandra A.C. Newman, Richard A. Baylis, Daniel L. Hess, Steven D. Griffith, Laura S. Shankman, Olga A. Cherepanova, Gary K. Owenscontrol. TUNEL staining was performed using the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, 30074; lot 180417). Cells were counter stained with DAPI (Thermo Fisher Scientific, D3571). Secondary antibodies

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CF®640R TUNEL Assay Apoptosis Detection Kit

CF®640R TUNEL Assay Apoptosis Detection Kit

CF®640R TUNEL Assay Apoptosis Detection Kit from Biotium, Inc.

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Biotium, Inc.

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CF®640R TUNEL Assay Apoptosis Detection Kit from Biotium, Inc.

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