Complement Component C5a ELISA DY2150 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemComplement Component C5a ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY2150
NCBI Full Gene Namecomplement C5
NCBI Gene AliasesC5D, C5a, C5b, CPAMD4, ECLZB
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
Detection TargetComplement Component C5a
ReactivityMouse
Detection Range15.60 - 1000 pg/mL
ApplicationsELISA
Quantity1 Kit (for 15 Plates)

Specifications/Features

Available conjugates: Sizes Available: 1 Kit (for 15 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Properdin deficiency or anti-properdin treatment ameliorates disease in the C3 gain-of-function mouse model of atypical haemolytic uraemic syndromeFrontiers in ImmunologyApril 28, 2026Grace Mallett, Ola Kamala, Isabel Y. Pappworth, Kate Smith-Jackson, Beth G. Gibson, Muhammad Asif, Harriet Denton, Charlotte Cox, Patrick Walsh, David Kavanagh, et al.detected using a DuoSet ELISA kit following the manufacturer’s instructions (Biotechne, R & D Systems, DY2150). Plates were then analysed on an LT-4500 plate reader (Lab Tech, UK) at OD450. In all cases, a standard

(2) House Dust Mite Nebulization Drives Alarmin and Complement Activation in a Murine Tracheal Air-Liquid Interface Culture SystemCellsOctober 14, 2025Janti Haj Ahmad, Philip Einwohlt, Mareike Ohms, Doris Wilflingseder, Jörg Köhlwas measured immediately. Mouse C5a levels were measured using a commercial ELISA kit (R&D Systems, #DY2150—Minneapolis, MN, USA); C3 concentrations were quantified using a kit from Hycult Biotech (HycultBiotech

(3) Directed-complement killing of Pseudomonas aeruginosa protects against lethal pneumoniaEBioMedicineOctober 1, 2025Pitiot A, Brandus B, Iserentant G, Rolin C, Servais JY, Fouquenet D, Chesnay A, Richert L, Briard B, Si-Tahar M, et al.measure anaphylatoxin C5a concentrations in the BAL and the lungs, we used a C5a mouse ELISA kit (#DY2150, R&D Systems, United States) following the manufacturer's instructions. Statistical analysis All in vitro tests

(4) Breast milk IgG engages the mouse neonatal immune system to instruct responses to gut antigensScienceAugust 14, 2025Meera K. Shenoy, Diane M. Rico, Alina K. Lorant, Hamadoun Touré, Shannon Gordon, Luke J. Milburn, Jeanette S. Schwensen, Madelyn E. Cabán, Meghan A. Kochat room temperature. C5a ELISA was performed by following the manufacturer instructions (Biotechne DY2150) using Duoset reagents kit (DY008B). For isotype and OVA-specific ELISA, plates were washed 5X with

(5) Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell miceBlood AdvancesMarch 25, 2025Anne Grunenwald, Julie Peliconi, Julien Lavergne, Margot Revel, Elodie Voilin, Tania Robe-Rybkine, Gilles Crambert, Jordan D. Dimitrov, Olivier Blanc-Brude, Lubka T. Roumeninaenzyme-linked immunosorbent assay (ELISA) Kit (ab157716; Abcam) and the Mouse Complement Component C5a DuoSet ELISA kit (DY2150; R&D Systems), according to the manufacturer’s recommendations. Absorbance was measured at 450 nm with

(6) Lung-delivered IL-10 mitigates Lung inflammation induced by repeated endotoxin exposures in male micePhysiological reportsFebruary 1, 2025Schwab AD, Wyatt TA, Schanze OW, Nelson AJ, Gleason AM, Duryee MJ, Mosley DD, Thiele GM, Mikuls TR, Poole JA.

(7) Highly efficient nucleic acid encapsulation method for targeted gene therapy using antibody conjugation systemMolecular therapy. Nucleic acidsDecember 10, 2024Hong S, Jeong SH, Han JH, Yuk HD, Jeong CW, Ku JH, Kwak C.complement analysis, C5a and C3a levels were measured using the Mouse Complement Component C5a DuoSet ELISA (DY2150, R&D Systems, Minneapolis, MN) and the Mouse Complement C3a ELISA Kit (Colorimetric) (NBP2-70037, Novus Biologicals

(8) Enhanced complement activation and MAC formation accelerates severe COVID-19Cellular and Molecular Life SciencesDecember 1, 2024Calder R. Ellsworth, Zheng Chen, Mark T. Xiao, Chaosi Qian, Chenxiao Wang, Mst Shamima Khatun, Shumei Liu, Mohammad Islamuddin, Nicholas J. Maness, Jose A. Halperin, et al.measured in duplicate using the following ELISA kits and dilutions. C5a ELISA 1:50 dilution (R&D systems, DY2150), C3a ELISA 1:10 dilution (R&D systems, NBP2-70,037), VEGF ELISA 1:20 (R&D systems, MMV00-1), and vWF

(9) Complement C5a Receptor Signaling Alters Stress Responsiveness and Modulates Microglia Following Chronic Stress ExposureBiological Psychiatry Global Open ScienceMay 1, 2024Hsiao-Jou Cortina Chen, Jereme G. Spiers, Titaya Lerskiatiphanich, Sandra E. Parker, Nickolas A. Lavidis, Jenny N. Fung, Trent M. Woodruff, John D. Leecomponent C5a enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (DY2150, R&D Systems). Levels of C5a in plasma samples were determined from a standard curve ranging from 15.6

(10) Inhibition of acute complement responses towards bolus-injected nanoparticles using targeted short-circulating regulatory proteinsNature nanotechnologyFebruary 1, 2024Li Y, Jacques S, Gaikwad H, Wang G, Banda NK, Holers VM, Scheinman RI, Tomlinson S, Moghimi SM, Simberg D.levels were measured according to manufacturers’ instructions using a mouse C5a DuoSet ELISA kit (cat. # DY2150) from R&D Systems and rat C5a ELISA kit (cat. # MBS160277) from MyBioSource. The plates were read with

(11) C5aR1 signaling triggers lung immunopathology in COVID-19 through neutrophil extracellular trapsThe Journal of clinical investigationJune 15, 2023Silva BM, Gomes GF, Veras FP, Cambier S, Silva GV, Quadros AU, Caetité DB, Nascimento DC, Silva CM, Silva JC, et al.to detect the concentration of C5a, factor Bb, and C3a using kits from R&D Systems (catalog numbers DY2150, NBP2-75243, and CTK-148, respectively), according to the manufacturer’s instructions. Virus stock

(12) Effects of C1-INH Treatment on Neurobehavioral Sequelae and Late Seizures After Traumatic Brain Injury in a Mouse Model of Controlled Cortical ImpactNeurotrauma ReportsMarch 21, 2023Min Chen, Quang M. Tieng, Jiaxin Du, Stephen R. Edwards, Dhiraj Maskey, Emil Peshtenski, David Reutenspreviously.17 C5a expression levels were determined using enzyme-linked immunosorbent assay (ELISA; #DY2150; R&D Systems, Minneapolis, MN). The measured concentration of C5a was normalized to a protein concentration,

(13) The role of plasminogen activator inhibitor-2 in pneumococcal meningitisActa neuropathologica communicationsOctober 29, 2022Teske NC, Engelen-Lee JY, Dyckhoff-Shen S, Pfister HW, Klein M, van de Beek D, Kirschning CK, Koedel U, Brouwer MC.

(14) CD55 Facilitates Immune Evasion by Borrelia crocidurae, an Agent of Relapsing FevermBioOctober 26, 2022Arora G, Lynn GE, Tang X, Rosen CE, Hoornstra D, Sajid A, Hovius JW, Palm NW, Ring AM, Fikrig E.

(15) Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cellsJournal of neuroinflammationJuly 14, 2022Llorián-Salvador M, Byrne EM, Szczepan M, Little K, Chen M, Xu H.

(16) Modulation of C5a-C5aR1 signaling alters the dynamics of AD progressionJournal of neuroinflammationJuly 11, 2022Carvalho K, Schartz ND, Balderrama-Gutierrez G, Liang HY, Chu SH, Selvan P, Gomez-Arboledas A, Petrisko TJ, Fonseca MI, Mortazavi A, et al.C5a concentration was determined with the R&D Systems Mouse Complement Component C5a DuoSet ELISA (DY2150) according to the manufacturer’s instructions, in triplicate. Optical density was read on SpectraMax Plus 384

(17) Endothelial expression of human amyloid precursor protein leads to amyloid β in the blood and induces cerebral amyloid angiopathy in knock-in miceThe Journal of biological chemistryJune 1, 2022Tachida Y, Miura S, Muto Y, Takuwa H, Sahara N, Shindo A, Matsuba Y, Saito T, Taniguchi N, Kawaguchi Y, et al.sensitive) Assay Kit (IBL-Japan) was used. A mouse complement component C5a DuoSet ELISA (catalog no.: DY2150; R&D Systems) was used to measure serum C5a in mice. RNA preparation For real-time PCR, spleens isolated

(18) Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesisCell reportsMay 31, 2022Ding P, Xu Y, Li L, Lv X, Li L, Chen J, Zhou D, Wang X, Wang Q, Zhang W, et al.

(19) A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injuryScientific reportsNovember 8, 2021Bongoni AK, Vikstrom IB, McRae JL, Salvaris EJ, Fisicaro N, Pearse MJ, Wymann S, Rowe T, Morelli AB, Hardy MP, et al.(HK216, Hycult Biotech) and for C5a using the Mouse Complement Activation Component C5a DuoSet ELISA kit (DY2150; R&D Systems, Minneapolis, MN) as per the manufacturer’s instructions. Plasma samples were also analysed

(20) The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung diseaseInternational immunopharmacologyNovember 1, 2021Mikuls TR, Gaurav R, Thiele GM, England BR, Wolfe MG, Shaw BP, Bailey KL, Wyatt TA, Nelson AJ, Duryee MJ, et al.

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Mouse Complement Component C5a DuoSet ELISA

Complement Component C5a ELISA from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Complement Component C5a ELISA from R&D Systems, a Bio-Techne Brand

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