Description
These kits may be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa. Labeled antibodies can be used for applications such as flow cytometry, microscopy, and immunohistochemistry. This kit is optimized for labeling 1 mg of a primary or secondary IgG antibody. This kit contains 3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C), 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C), 25 mL PBS (store at 4 °C), 0.5 mL Ultra Pure Water (store at 4 °C), 3 x Pierce® Zeba™ Desalting Spin Columns (store at 4 °C), Pierce Zeba Desalting Spin Column instructions, Protocol for VRDye Protein Labeling Kit – High MW, and a Quick Reference Guide