Fig 1: Effects of nephrectomy (Nx) on the expression of Fndc5 and myostatin in mouse tissues. (A) Total RNA was extracted from the gastrocnemius muscles (GA), femurs, visceral white adipose tissue (WAT), liver, and kidneys of mice 8 weeks after the last Nx or sham surgery. A real-time PCR analysis of Fndc5, myostatin, and 18S rRNA was performed. Data represent the mean ± standard error of the mean (n = 6 mice in each group). (B,C) Total RNA was extracted from C2C12 myotubes 24 h after exposure to 10-4 M hydrogen peroxide (H2O2), 20 ng/mL TNF-a, 10-8 M PTH 1-34 (PTH), 10-8 M 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), 200 µg/mL BSA, 200 µg/mL AGE3, or 10 and 100 ng/mL IL-6, and real-time PCR analysis of Fndc5 and 18S rRNA was performed. Data represent the mean ± standard error of the mean (n = 4 sample in each group). Cont, control. **P < 0.01. (D) Total RNA was extracted from C2C12 myotubes 24 h after the 20 ng/mL TNF-a treatment with or without 100 nM irisin, and real-time PCR analysis of Fndc5 and 18S rRNA was performed. Data represent the mean ± standard error of the mean (n = 6 sample in each group). (E) Serum samples were collected from mice 8 weeks after the last Nx or sham surgery. Serum TNF-a and IL-6 levels were analysed. Data represent the mean ± standard error of the mean (n = 6 mice in each group). *P < 0.05 and **P < 0.01.