Description
Intended Uses: The Murine TNF-alpha ELISA is to be used for the in-vitro quantitative determination of murine tumor necrosis factor-alpha (mTNFalpha) in murine serum, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant murine TNF-alpha.
Principle of the Assay: The murine TNF-alpha Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for mTNFalpha has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated polyclonal antibody specific for mTNFalpha are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen