Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to AA has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated AA and incubated. A competitive inhibition reaction is launched between AA (Standards or samples) and HRP-conjugated AA with the pre-coated antibody specific for AA. The more amount of AA in samples, the less antibody bound by HRP-conjugated AA. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured