Description
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MIF concentrations in Canine serum, plasma and other body fluids. Using Purified Canine MIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIF and MIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve