Description
Principle of the assay: This test kit applies competition method to detect the content of Mouse Free Tri-iodothyronine Indes (Free-T3). Add samples to enzyme well which has been pre-coated with antibodies, then add recognition antigen labeled by horse radish peroxidase (HRP); after been incubated 1 hour at 37 0C, both compete with solid phase antigen and formed immune complex; after been washing by PBST, the combined HRP catalyses TMB (Tetramethyl benzidine) into blue, and turns into yellow by the action of acid; it has absorption peak under 450nm wavelength, and its absorbance is negatively correlated with antigen density of sample