Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated IGF2. The competitive inhibition reaction is launched between with HRP-conjugated IGF2 and IGF2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IGF2 in the sample. The color development is stopped and the intensity of the color is measured