Description
Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TNFsR I polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TNFsRI polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNFsRI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNFsRI can be calculated.
Background: Tumor necrosis factor receptor superfamily member 1A is a protein that in humans is encoded by the TNFRSF1A gene, which chromosome 12p13.2. It is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. Chan et al. (2000) found that, in contrast, the p60 and p80 TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand