Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ox-HDL. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Ox-HDL. The competitive inhibition reaction is launched between with pre-coated Ox-HDL and Ox-HDL in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Ox-HDL in the sample. The color development is stopped and the intensity of the color is measured