Description
Intended Uses: This human coagulation Factor XI antigen assay is intended for the quantitative determination of total Factor XI antigen in human plasma. For research use only.
Principle of the Assay: Human Factor XI will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XI and XIa will react with the antibody on the plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor XI. Color development is proportional to the concentration of Factor XI in the samples.
Background: Factor XI is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor XIa [1]. Factor XI consists of two identical monomers and circulates in plasma in complex with kininogen [2]. Factor XI is activated by Factor XIIa and converts Factor IX to Factor IXa during the intrinsic pathway of the coagulation cascade [3]