Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Apelin 12 (also known as AP12) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human AP12 antibody. AP12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human AP12 Antibody is added and binds to AP12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AP12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human AP12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm