Description
Introduction: This Quantitative Sandwich ELISA kit is in tended to determinate DEFA1 concentrations in Fish serum, plasma and other body fluids. Using Purified Fish DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve