Description
Intended Uses: This human plasminogen total assay is for the quantitative determination of total plasminogen and plasmin in human plasma, serum, urine, cell culture media, or tissue extracts. For research use only.
Principle of the Assay: Human plasminogen will bind to the capture antibody provided for coating on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
Background: Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis [1]