Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to CTXII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CTXII and unlabeled CTXII (Standards or samples) with the pre-coated antibody specific to CTXII. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CTXII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CTXII in the sample