Description
Intended Uses: The Rat TNFa ELISA is to be used for the in-vitro quantitative determination of rat Tumor Necrosis Factor. The assay will recognize both natural and recombinant Rat TNF-a.
Principle of the Assay: The Rat TNF-a Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for rat TNF-a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF-a concentrations and unknowns are pipetted into these wells. During the first incubation, the rat TNF-a antigen and a biotinylated monoclonal antibody specific for rat TNF-a are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of rat TNF-a present in the samples