Description
Intended Uses: The Murine IL-10 ELISA is to be used for the in-vitro quantitative determination of murine interleukin-10 (mIL-10) in murine serum, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant mIL-10.
Principle of the Assay: The murine IL-10 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A polyclonal antibody specific for mIL-10 has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated monoclonal antibody specific for mIL-10 are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen