Description
Intended Uses: For the qualitative determination of activated Porcine Smooth Muscle Antibody (Anti-SM) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.
Principle of the Assay::The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Porcine Anti-SM in samples. Add sample to wells pre-coated with one Porcine Smooth Muscle antigen, at same time add HRP-conjugated Porcine Smooth Muscle second antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Porcine Anti-SM in the samples is then determined by comparing the O.D. of the samples to the CUT OFF