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Assay Kits
Assay Kit
CFSE Cell Division Tracker Kit
CFSE Cell Division Tracker Kit from BioLegend
(1)
Write a review
Citations:
(130)
Supplier Page
Supplier Page from
BioLegend for
CFSE Cell Division Tracker Kit
Description
CFSE Cell Division Tracker Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CFSE Cell Division Tracker Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423801
Quantity
1 kit
Applications
Intracellular Staining for Flow Cytometry (ICFC), in-vivo Cell Tracking
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (130)
(1)
Peripherally derived myeloid cells induce disease-dependent phenotypic changes in microglia
Front Cell Neurosci
December 14, 2023
Estrid Thougaard, Brianna Carney, Agnieszka Wlodarczyk, Roberta Brambilla, Kate Lykke Lambertsen
myeloid cells
were labeled with carboxyfluorescein succinimidyl ester (
CFSE
) (2 μM dilution in PBS,
BioLegend
, #
423801
) for 10 min at 37°C. Then 50,000
myeloid cells
in 500 μl media were plated in the 24-well plates and
(2)
Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases
J Immunol Res
December 8, 2023
Wai Chung Wu, Carol Shiu, Tak Keung Tong, Shui On Leung, Chin Wai Hui
days. K562 and MSCs (target cells) were counted and 1 × 106 cells were labeled with 100 µM of
CFSE
(#
423801
,
BioLegend
) for 10 min at 37°C. Labeled target cells were washed and resuspended in complete ATCC-modified
(3)
Macrophage infiltration in 3D cancer spheroids to recapitulate the TME and unveil interactions within cancer cells and macrophages to modulate chemotherapeutic drug efficacy
BMC Cancer
December 7, 2023
Khushwant Singh, Pramod K. Gautam
130–123-310) antibodies were used. CalcineAM (Cat. No. 13529), Mitospy (Cat. No. 50–207-9911),
CFSE
(Cat. No.
423801
) were purchased from
Biolegend
(San Diego, USA) and DAPI Solution (Cat. No.
62247
) and Cobalt(II) chloride
(4)
Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis
STAR Protocols
December 1, 2023
Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu,
et al.
incubate the isolated CD8+ T cells with reagents from a
CFSE Cell Division Tracker Kit
(
BioLegend
,
423801
) according to the manufacturer’s instructions (https://www.biolegend.com/en-us/products/cfse-cell-division-tracker-kit-9396)
(5)
Breast cancer stem cells generate immune-suppressive T regulatory cells by secreting TGFβ to evade immune-elimination
Discov Oncol
December 1, 2023
Sumon Mukherjee, Sourio Chakraborty, Udit Basak, Subhadip Pati, Apratim Dutta, Saikat Dutta, Dia Roy, Shruti Banerjee, Arpan Ray, Gaurisankar Sa,
et al.
experimental set. Similarly, proliferation of CD4+ T-cells was evaluated by
CFSE
(
Biolegend
, Cat. No.
423801
) dilution
assay
. For
CFSE
assay
, cells were stained with 5µM
CFSE
in PBS for 20 min at 37 °C. The cells
(6)
Primary explants of the postnatal thymus allow the expansion of clonogenic thymic epithelial cells that constitute thymospheres
Stem Cell Research & Therapy
October 31, 2023
Juan M. Ocampo-Godinez, Jose L. Gonzalez-Quiroz, Hector Cote-Palafox, Elizabeth George, Jael A. Vergara-Lope Nuñez, Guillermo Villagomez-Olea, Febe C. Vazquez-Vazquez, Edgar O. Lopez-Villegas, Gloria Leon-Avila, Maria L. Dominguez-Lopez,
et al.
of IL25 (Additional file 1: Fig. S7a-d). The proliferation
assay
was performed with
CFSE
BioLegend
® (
423801
) cell division tracker at 5 mM on day 5 when a considerable number of cells emerged from the explants.
(7)
Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing
Research (Wash D C)
June 28, 2023
Qing Liu, Qiaoyun Long, Jiayu Zhao, Wenjie Wu, Zexin Lin, Wei Sun, Ping Gu, Tuo Deng, Kerry Martin Loomes, Donghai Wu,
et al.
EDTA
). Mouse spleen cells were incubated with 5 μM carboxyfluorescein succinimidyl ester (
BioLegend
, #
423801
) in PBS for 30 min at 37 °C in water batch and then cocultured with isolated primary
adipocytes
from
(8)
Cell-cell interactome of the hematopoietic niche and its changes in acute myeloid leukemia
iScience
June 16, 2023
Sarah Ennis, Alessandra Conforte, Eimear O’Reilly, Javid Sabour Takanlu, Tatiana Cichocka, Sukhraj Pal Dhami, Pamela Nicholson, Philippe Krebs, Pilib Ó Broin, Eva Szegezdi
and recombinant proteins" ,, TGFb1,
Peprotech
,Cat. Number:
100-21-10ug
CFSE
,
Biolegend
,Cat. Number:
423801
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, Chromium
(9)
Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo models
Molecular Therapy - Nucleic Acids
June 13, 2023
Hadi Bayat, Mohammad Hossein Pourgholami, Saeid Rahmani, Safura Pournajaf, Seyed Javad Mowla
C6 cells were labeled with 1 μL/mL carboxyfluorescein diacetate succinimidyl ester (
BioLegend
; Cat. #
423801
) according to the manufacturer’s recommendations. The labeled cells were plated at a number of 1.5 ×
(10)
Low responsiveness of peripheral lymphocytes in extraparenchymal neurocysticercosis
PLOS Neglected Tropical Diseases
June 1, 2023
Matthew L. Romo, Rocio Osorio, Andrea Toledo, Roger Carrillo-Mezo, Ricardo Valdez, Marta C. Romano, Edda Sciutto, Gladis Fragoso, Agnès Fleury
by
staining
10 x106 PBMC with carboxyfluorescein diacetate succinimidyl ester (
CFSE
,
Biolegend
Cat.
423801
) at 5μM diluted in RPMI-1640 for 5 minutes then washed twice with RPMI-1640 medium supplemented with
(11)
Isolation of slow-cycling cancer cells from lung patient-derived xenograft using carboxyfluorescein-succinimidyl ester retention-mediated cell sorting
STAR Protocols
June 1, 2023
Jaebeom Cho, Hye-Young Min, Ho-Young Lee
,
Miltenyi Biotec
,
130-093-237
gentleMACS Dissociator
,
Miltenyi Biotec
,
130-093-235
CFSE Cell Division Tracker Kit
,
BioLegend
,
423801
,, Software and algorithms,Software and algorithms,Software and algorithms ,, FACSDiva software,BD
(12)
Dual inhibition of IDO1/TDO2 enhances anti-tumor immunity in platinum-resistant non-small cell lung cancer
Cancer & metabolism
May 24, 2023
Wu C, Spector SA, Theodoropoulos G, Nguyen DJM, Kim EY, Garcia A, Savaraj N, Lim DC, Paul A, Feun LG,
et al
.
washed and incubated with 1 µm of carboxyfluorescein succinimidyl ester (
CFSE
) (
BioLegend
, cat no.
423801
) at 37 °C for 7 min then thoroughly washed with
staining
buffer before
in vitro
co-culture
. The proliferation
(13)
Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression
iScience
May 19, 2023
Dou Ye, Suqing Qu, Yinxiang Yang, Zhaoyan Wang, Qian Wang, Weipeng Liu, Fan Zhang, Qian Guan, Xiaohua Wang, Jing Zang,
et al.
resuscitated and treated with 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester (
CFSE
, 0.1 μM, Cat. #
423801
,
Biolegend
, San Diego, California, USA) to assess cell division and proliferation, and were set up without
(14)
An Ionic Liquid Ablation Agent for Local Ablation and Immune Activation in Pancreatic Cancer
Advanced Science
April 1, 2023
Junming Huang, Meng Wang, Fu Zhang, Shiyi Shao, Zhuo Yao, Xinyu Zhao, Qida Hu, Tingbo Liang
anti‐CD11c‐pc5.5 (
560584
), and anti‐CD206‐AF647 (
565250
) antibodies were purchased from
BD Biosciences
.
CFSE
(
423801
), anti‐
CD25
‐AF700 (
102024
), and anti‐
F4/80
‐PE‐Cy7 (
157308
) antibodies were purchased from
Biolegend
(15)
T-cells engineered with a novel VHH-based chimeric antigen receptor against CD19 exhibit comparable tumoricidal efficacy to their FMC63-based counterparts
Frontiers in Immunology
February 16, 2023
Fatemeh Nasiri, Pooria Safarzadeh Kozani, Fatemeh Rahbarizadeh
was analyzed using the carboxyfluorescein succinimidyl ester (
CFSE
;
BioLegend
, San Diego, CA; Cat No.
423801
)
cell staining
assay
(22). Briefly, 106 - 107 effector
T cells
were labeled with 1 μL of 5 mM
CFSE
per
(16)
Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl
Environmental Science & Technology
January 31, 2023
Riley M. Behan-Bush, Jesse N. Liszewski, Michael V. Schrodt, Bhavya Vats, Xueshu Li, Hans-Joachim Lehmler, Aloysius J. Klingelhutz, James A. Ankrum
and plated for
co-culture
. PBMCs were stained with
CFSE
Cell Division Tracker dye (
BioLegend
; Cat: #
423801
) for 15 min. Any unbound
CFSE
dye was quenched with RPMI (15% FBS). Two-hundred fifty thousand cells
(17)
A drug screening to identify novel combinatorial strategies for boosting cancer immunotherapy efficacy
Journal of translational medicine
January 13, 2023
Zhang Z, Wang G, Zhong K, Chen Y, Yang N, Lu Q, Yuan B, Wang Z, Li H, Guo L,
et al
.
drugs plus
B7-H3
CAR
T cells
respectively.
B7-H3
CAR
T cells
were stained with
CFSE
(
Biolegend
, Cat#
423801
). The HCT116 spheroids were stained with Calcein/PI (Beyotime, Cat# C2015M). Images were captured at
(18)
FOXP3 Isoforms Expression in Cervical Cancer: Evidence about the Cancer-Related Properties of FOXP3Δ2Δ7 in Keratinocytes
Cancers (Basel)
January 5, 2023
Natalia Garcia-Becerra, Marco Ulises Aguila-Estrada, Luis Arturo Palafox-Mariscal, Georgina Hernandez-Flores, Adriana Aguilar-Lemarroy, Luis Felipe Jave-Suarez
cell index change over time. 2.7. Cell Division Tracking CSFE Cell Division Tracker Kit (Cat. No.
423801
,
BioLegend
, San Diego, CA, USA) was used for cell division analysis. CSFE (carboxyfluorescein succinimidyl
(19)
Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus Kidney
Mediators of inflammation
January 1, 2023
Wang Z, Shen J, Ye K, Zhao J, Huang S, He S, Qin Y, Meng L, Wang J, Song J.
viability dye eFluor 660 (
Invitrogen
, #
65-0864-14
), FerroOrange (Dojindo, #F374),
CFSE
(
Biolegend
, #
423801
),
IL6
-PE (
Biolegend
, #
504503
),
B220
-FITC (
Biolegend
, #
103205
),
Ki67
-APC (
Biolegend
, #
350514
),
SLC7A11
(20)
Cadonilimab, a tetravalent PD-1/CTLA-4 bispecific antibody with trans-binding and enhanced target binding avidity
mAbs
January 1, 2023
Pang X, Huang Z, Zhong T, Zhang P, Wang ZM, Xia M, Li B.
(CHO-K1-PD1-CTLA4 cells constructed by Akeso Biopharma) were collected and labeled with
CFSE
(
Biolegend
, Cat.:
423801
) (2.5 μM).
Macrophages
and target cells were co-cultured in DMEM-CM with indicated antibodies. DMEM-CM
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Reviews
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(1)
9/22/2017
CFSE Staining Kit for PBMC staining
Max Hübner
To assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.
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CFSE Cell Division Tracker Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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